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  4. Recombinant Human COMM domain-containing protein 1 (COMMD1)

Recombinant Human COMM domain-containing protein 1 (COMMD1)

  • 中文名称：
    Recombinant Human COMM domain-containing protein 1(COMMD1)
  • 货号：
    CSB-EP818712HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    COMMD1
  • Uniprot No.：
  • 别名：
    C2orf5; COMD1; COMD1_HUMAN; COMM domain-containing protein 1; COMMD1; Copper metabolism (Murr1) domain containing 1; Copper metabolism domain containing 1; Copper metabolism gene MURR1; Copper metabolism MURR1 domain-containing protein 1; MGC27155; MURR1; Protein Murr1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    48.0kDa
  • 表达区域：
    1-190aa
  • 氨基酸序列
    AAGELEGGKPLSGLLNALAQDTFHGYPGITEELLRSQLYPEVPPEEFRPFLAKMRGILKSIASADMDFNQLEAFLTAQTKKQGGITSDQAAVISKFWKSHKTKIRESLMNQSRWNSGLRGLSWRVDGKSQSRHSAQIHTPVAIIELELGKYGQESEFLCLEFDEVKVNQILKTLSEVEESISTLISQPN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activity and dissociates CAND1 from CUL1 and CUL2. Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Involved in the regulation of membrane expression and ubiquitination of SLC12A2. Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes. Promotes CFTR cell surface expression through regulation of its ubiquitination. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1. Negatively regulates neuroblastoma G1/S phase cell cycle progression and cell proliferation by stimulating ubiquitination of NF-kappa-B subunit RELA and NF-kappa-B degradation in a FAM107A- and actin-dependent manner.
  • 基因功能参考文献：
    1. Sp1 constitutively regulates the basal expression of the COMMD1 gene in human epithelial cell lines. PMID: 29336469
    2. This study has thus revealed a novel nuclear complex of F-actin, DRR1 and COMMD1 that is involved in NF-kappaB degradation and cell cycle suppression in neuroblastoma cells. PMID: 28604741
    3. these results identify COMMD1 and an E2F-metabolic pathway as key regulators of osteoclastogenic responses under pathological inflammatory conditions PMID: 28723554
    4. The COMMD1 downregulation by miR-205 promotes tumor development by modulating a positive feedback loop that amplifies inflammatory- and stemness-associated properties of cancer cells. PMID: 26586569
    5. COMMD1 plays a critical role in the termination of NF-kappaB activity and the control of pro-inflammatory and pro-labor mediators. PMID: 26733542
    6. COMMD1 expression is associated with poor prognosis in diffuse large B-cell lymphoma PMID: 24625556
    7. COMMD1 is identified as a novel regulator of misfolded protein aggregation. PMID: 24691167
    8. COMMD1 is directly linked to early endosomes through its interaction with a protein complex containing CCDC22, CCDC93, and C16orf62. PMID: 25355947
    9. COMMD1 is acetylated by p300 and that acetylation protects COMMD1 from XIAP-mediated proteosomal degradation PMID: 25074812
    10. IkappaB-alpha protein was stabilized by COMMD1, which attenuated NF-kappaB signaling during Toll-like receptor ligand and tumor necrosis factor alpha treatment and enhanced HIV-1 latency in latently HIV-1-infected cells. PMID: 25520503
    11. These data demonstrate the anti-inflammatory properties of COMMD1 in bronchial epithelial cells and open new therapeutic avenues in cystic fibrosis. PMID: 23892095
    12. Placental COMMD1 expression is increased in women with severe preeclampsia compared to that found in women with normal pregnancies. PMID: 23364987
    13. The role of COMMD1 in copper metabolism and it structure and function are discussed. PMID: 23677795
    14. The results indicate a role for COMMD1 in the regulation of NKCC1 membrane expression and ubiquitination. PMID: 23515529
    15. No major role can be attributed to Atox1 and COMMD in the pathophysiology or clinical variation of Wilson disease. PMID: 22677543
    16. Clusterin and COMMD1 independently regulate degradation of the mammalian copper ATPases ATP7A and ATP7B. PMID: 22130675
    17. These results suggest that COMMD1 downregulates deltaENaC activity by reducing deltaENaC surface expression through promoting internalization of surface deltaENaC to an intracellular recycling pool, possibly via enhanced ubiquitination. PMID: 21741370
    18. Data show that COMMD1 interacts with CFTR. This interaction promotes CFTR cell surface expression as assessed by biotinylation experiments in heterologously expressing cells through regulation of CFTR ubiquitination. PMID: 21483833
    19. We argue that COMMD1 participates in the normal disposition of copper within the hepatocyte and we speculate about that role. PMID: 21275100
    20. COMMD1 as a novel protein regulating SOD1 activation and associate COMMD1 function with the production of free radicals. PMID: 20595380
    21. report a single novel, putative mutation in COMMD1 in one Wilson disease (WD) patient with atypical features; absence of any other prospective mutations among 108 patients suggests that COMMD1 variants are not major contributors towards WD phenotypes PMID: 20550661
    22. Elevated levels of sCLU promote prostate cancer cell survival by facilitating degradation of COMMD1 and I-kappaB, thereby activating the canonical NF-kappaB pathway. PMID: 20068069
    23. COMMD1 has a role in conjunction with HSP90beta/HSP70 in the ubiquitin and O(2)-independent regulation of HIF-1alpha PMID: 19802386
    24. These data identify a new role for COMMD1 in regulating the nuclear/nucleolar distribution of RelA PMID: 20048074
    25. No mutations in the MURR1 gene, including the intron-exon boundaries, were identified in a total of 23 patients with non-Wilsonian hepatic copper toxicosis PMID: 12547404
    26. findings reveal involvement of Murr1 in the defined pathway of hepatic biliary copper excretion, suggest a mechanism for Murr1 function in this process, and provide evidence in support of the proposed role of the MURR1 gene in hepatic copper toxicosis PMID: 12968035
    27. MURR1 was detected in different tissues and cell lines; in cell lines it was found both in cytosol and membrane preparations; in some cells MURR1 was associated with a vesicular compartment diffusely localized throughout the cell PMID: 14568250
    28. Murr1 is a novel regulator of delta ENaC PMID: 14645214
    29. Murr1, a gene product known previously for its involvement in copper regulation, inhibits HIV-1 growth in unstimulated CD4+ T cells PMID: 14685242
    30. XIAP functions through MUUR1 to regulate copper homeostasis. PMID: 14685266
    31. 3 intronic base pair changes, 1 new sequence variation & 2 known polymorphisms were detected, including the GAT/GAC heterozygous state at Asn 164 in 24% of the patients. GAT/GAC heterozygosity at Asn 164 is associated with earlier onset of Wilson disease. PMID: 15205742
    32. MURR1/COMMD1 functions in the nucleus by affecting the association of NF-kappaB with chromatin PMID: 15799966
    33. COMMD1 is not a significant contributor to Wilson-like copper storage disorders in humans. PMID: 16283886
    34. These data support the significance of COMMD protein-protein interactions and provide new mechanistic insight into the function of this protein family in NF-kappaB signalling. PMID: 16573520
    35. The solution structure of the N-terminal domain of COMMD1 (N-COMMD1, residues 1-108), is presented. PMID: 17097678
    36. COMMD1 accelerates the ubiquitination and degradation of NF-kappaB subunits through its interaction with a multimeric ubiquitin ligase containing Elongins B and C, Cul2 and SOCS1 (ECS(SOCS1)). PMID: 17183367
    37. COMMD1 specifically binds copper as Cu(II) in 1:1 stoichiometry & does not bind other divalent metals. Fluorescence studies of single point mutants of the full-length protein revealed the involvement of M110 in addition to H134 in direct Cu(II) binding. PMID: 17309234
    38. Implicate COMMD1 in the pathogenesis of Wilson's disease and indicate that COMMD1 exerts its regulatory role in copper homeostasis through the regulation of ATP7B stability. PMID: 17919502
    39. the ability to promote Lys(63)-mediated polyubiquitination of COMMD1 is a novel property of ARF independent of p53 PMID: 18305112
    40. COMMD1 expression is controlled primarily by protein ubiquitination PMID: 18795889
    41. COMMD1 is a scaffold protein in a distinct sub-compartment of endocytic pathway and offer first clues to its role as a regulator of structurally unrelated membrane transporters. PMID: 18940794
    42. Data suggest that translocation of ATP7B takes place independently of Rab7-regulated endosomal traffic, and that Murr1 plays a role in a later step of the copper excretion pathway but is not involved in the translocation of the Wilson disease protein. PMID: 18974300

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Endosome membrane. Cytoplasmic vesicle. Early endosome. Recycling endosome. Note=Shuttles between nucleus and cytosol. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins.
  • 组织特异性：
    Ubiquitous. Highest expression in the liver, with lower expression in brain, lung, placenta, pancreas, small intestine, heart, skeletal muscle, kidney and placenta. Down-regulated in cancer tissues.
  • 数据库链接：

    HGNC: 23024

    OMIM: 607238

    KEGG: hsa:150684

    STRING: 9606.ENSP00000308236

    UniGene: Hs.468702