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  4. Recombinant Human Calcium and integrin-binding protein 1 (CIB1)

Recombinant Human Calcium and integrin-binding protein 1 (CIB1)

  • 中文名称：
    Recombinant Human Calcium and integrin-binding protein 1(CIB1),Yeast
  • 货号：
    CSB-YP857460HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Calcium and integrin-binding protein 1(CIB1),Yeast
  • 货号：
    CSB-EP857460HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Calcium and integrin-binding protein 1(CIB1),Yeast
  • 货号：
    CSB-EP857460HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Calcium and integrin-binding protein 1(CIB1),Yeast
  • 货号：
    CSB-BP857460HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Calcium and integrin-binding protein 1(CIB1),Yeast
  • 货号：
    CSB-MP857460HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CIB1
  • Uniprot No.：
  • 别名：
    Calcium and integrin binding 1 (calmyrin); Calcium and integrin binding protein 1; Calcium and integrin-binding protein 1; Calcium- and integrin-binding protein; Calmyrin; CIB; CIB1; CIB1_HUMAN; CIBP; DNA PKcs interacting protein; DNA-dependent protein kinase interacting protein; DNA-PK interaction protein; DNA-PKcs-interacting protein; Human Snk interacting protein 2 to 28 mRNA complete cds; Kinase interacting protein; Kinase-interacting protein 1; Kinase-interacting protein; KIP; KIP1; PRKDCIP; SIP2 28; SIP2-28; SNK interacting protein 2 to 28; SNK-interacting protein 2-28
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-191
  • 氨基酸序列
    GGSGSRLSK ELLAEYQDLT FLTKQEILLA HRRFCELLPQ EQRSVESSLR AQVPFEQILS LPELKANPFK ERICRVFSTS PAKDSLSFED FLDLLSVFSD TATPDIKSHY AFRIFDFDDD GTLNREDLSR LVNCLTGEGE DTRLSASEMK QLIDNILEES DIDRDGTINL SEFQHVISRS PDFASSFKIV L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-binding protein that plays a role in the regulation of numerous cellular processes, such as cell differentiation, cell division, cell proliferation, cell migration, thrombosis, angiogenesis, cardiac hypertrophy and apoptosis. Involved in bone marrow megakaryocyte differentiation by negatively regulating thrombopoietin-mediated signaling pathway. Participates in the endomitotic cell cycle of megakaryocyte, a form of mitosis in which both karyokinesis and cytokinesis are interrupted. Plays a role in integrin signaling by negatively regulating alpha-IIb/beta3 activation in thrombin-stimulated megakaryocytes preventing platelet aggregation. Up-regulates PTK2/FAK1 activity, and is also needed for the recruitment of PTK2/FAK1 to focal adhesions; it thus appears to play an important role in focal adhesion formation. Positively regulates cell migration on fibronectin in a CDC42-dependent manner, the effect being negatively regulated by PAK1. Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. Down-regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Involved in sphingosine kinase SPHK1 translocation to the plasma membrane in a N-myristoylation-dependent manner preventing TNF-alpha-induced apoptosis. Regulates serine/threonine-protein kinase PLK3 activity for proper completion of cell division progression. Plays a role in microtubule (MT) dynamics during neuronal development; disrupts the MT depolymerization activity of STMN2 attenuating NGF-induced neurite outgrowth and the MT reorganization at the edge of lamellipodia. Promotes cardiomyocyte hypertrophy via activation of the calcineurin/NFAT signaling pathway. Stimulates calcineurin PPP3R1 activity by mediating its anchoring to the sarcolemma. In ischemia-induced (pathological or adaptive) angiogenesis, stimulates endothelial cell proliferation, migration and microvessel formation by activating the PAK1 and ERK1/ERK2 signaling pathway. Promotes also cancer cell survival and proliferation. May regulate cell cycle and differentiation of spermatogenic germ cells, and/or differentiation of supporting Sertoli cells.; Plays a regulatory role in angiogenesis and tumor growth by mediating PKD/PRKD2-induced vascular endothelial growth factor A (VEGFA) secretion.; (Microbial infection) Involved in keratinocyte-intrinsic immunity to human beta-papillomaviruses (HPVs).
  • 基因功能参考文献：
    1. In patients with chronic stable ischemic heart failure, the diagnostic value of urine CIB1 outperforms that of serum pro-BNP PMID: 29028109
    2. In summary, as a multifunctional protein, CIB1 serving not only as a Ca2+ modulating protein, most surprisingly, but also as an important potential tumor promoting factor. [review] PMID: 29017172
    3. Results suggest that interaction of CIB1 with alphaIIb is one of the early events occurring during outside-in signaling. Furthermore, CIB1 recruits FAK to the alphaIIbbeta3 complex at the filopodia where FAK is activated, which in turn activates c-Src, resulting in propagation of outside-in signaling leading to platelet spreading. PMID: 28542214
    4. we found that ectopic expression of oncogenic KRas and HRas in cells resulted in elevated CIB1 expression. We previously described the Ca(2+)-myristoyl switch function of CIB1, and its ability to facilitate agonist-induced plasma membrane localisation of sphingosine kinase 1 (SK1), a location where SK1 is known to elicit oncogenic signalling. PMID: 27941888
    5. a large subset of TNBCs depend on CIB1 for cell survival and tumor growth, independent of CIB1 expression levels. PMID: 26105795
    6. mRNA and protein expression levels of CIB1 were decreased in the oligoasthenozoospermia patients.CIB1 may be involved in the pathogenesis of oligoasthenozoospermia by the CDK1 signaling pathway. PMID: 24464679
    7. High serum CIB1 levels are associated with pancreatic cancer.s PMID: 24585405
    8. KIP binding to TRF2 enhances the telomere-binding activity of TRF2, suggesting that KIP acts as a positive regulator of TRF2 function. PMID: 25012820
    9. these studies suggested that Kaposi's sarcoma-associated herpesvirus utilizes CIB1 as one of the key molecule(s) to coordinate and sustain the EphA2 mediated signaling involved in its entry PMID: 24550731
    10. CIB1a is a novel mediator of PKD2-driven carcinogenesis. PMID: 23503467
    11. we present data that show that CIB1 binds to seven additional alpha-integrin CT peptides, bringing the total number of alpha-integrins that can associate with CIB1 to eight. PMID: 24011356
    12. Results indicate that CIB1 is uniquely positioned to regulate PI3K/AKT and MEK/ERK signaling and that simultaneous disruption of these pathways synergistically induces a nuclear GAPDH-dependent cell death. PMID: 22964641
    13. The CIB1 and calcineurin expression was increased in AF atrial tissue and was related to the type of AF. This finding suggests that CIB1 may be involved in the pathogenesis of AF in VHD patients. PMID: 22547769
    14. results suggest that CIB1 positively regulates cell migration and is necessary for the recruitment of FAK to the focal adhesions. Furthermore, CIB1-induced cell migration is dependent on MAP kinase signaling and its function is attenuated by PAK1 PMID: 21748785
    15. CIB1 may be used as a novel prognostic factor and possibly an attractive therapeutic target for hepatocellular carcinoma. PMID: 21857112
    16. CaMy1 via SCG10 couples Ca(2+) signals with the dynamics of microtubules during neuronal outgrowth in the developing brain. PMID: 21215777
    17. analysis of Ca2+-CIB1 and Mg2+-CIB1 and their interactions with the platelet integrin alphaIIb cytoplasmic domain PMID: 21388953
    18. Calcium- and integrin-binding protein 1 regulates microtubule organization and centrosome segregation through polo like kinase 3 during cell cycle progression. PMID: 20951827
    19. content of DNA-PKcs was significantly higher in A549 cells than in H1299 cells PMID: 19923078
    20. CIB1 may be a potential biomarker and target for therapeutic intervention of breast cancer. PMID: 20473878
    21. CIB1 interacts with SK1 in a Ca(2+)-dependent manner at the previously identified "calmodulin-binding site" of SK1. PMID: 19854831
    22. binds NBR1 protein PMID: 11856312
    23. describes regions within CIB and alpha(IIb) that interact with one another PMID: 12023286
    24. An association between GPIIb/IIIa and calcium- and integrin-binding protein (CIB) is required for the process of platelet spreading. PMID: 12714504
    25. CIB regulates platelet spreading through the regulation of FAK activation. PMID: 12881299
    26. CIB may exist in multiple structural and metal ion-bound states in vivo which may play a role in its regulation of target proteins such as platelet integrin. PMID: 14992593
    27. KIP has a role in telomere length maintenance and regulation PMID: 15190070
    28. crystallographic structure of CIP1, an EF-hand-containing protein PMID: 15574431
    29. calmyrin may be involved in the pathogenesis of AD. PMID: 15885068
    30. Data indicate that although calmyrin forms stable covalent dimers in vitro, it most probably functions as a monomer in vivo. PMID: 15933764
    31. CIB1 as a key regulator of PAK1 activation and signaling. PMID: 16061695
    32. CIB1 appears to inhibit integrin activation by competing with talin for binding to alphaIIbbeta3, thus providing a model for tightly controlled regulation of alphaIIbbeta3 activation. PMID: 16418530
    33. CIB1 is a ubiquitously expressed activating and inhibiting protein ligand of the InsP(3)R PMID: 16723353
    34. Data report the structural characteristics of calcium- and integrin-binding protein 1 in solution and the mechanistic details of its interaction with a synthetic peptide derived from the alphaIIb integrin cytoplasmic domain. PMID: 16825200
    35. communication between the paired EF-hand domains as well as between the N- and C-lobes of CIB1 is distinct from the ancestral proteins calmodulin and troponin C, which might be important for the unique function of CIB1 in numerous biological processes PMID: 17516631
    36. The function of CIB1 on InsP3-evoked Ca2+ release from the ER is most likely mediated by its interaction with the InsP3 receptor. Thus, CIB1 seems to be an inhibitor of InsP3-dependent Ca2+ release in vivo. PMID: 18627437
    37. Characterization of calcium- and integrin-binding protein 1 (CIB1) knockout platelets: potential compensation by CIB family members. PMID: 18989529
    38. data identify low affinity (K(d), 10(-2)M) Ca(2+) binding events that influence the structures of the N- and C-terminal extensions of CIB1 under high (300 mM) Ca(2+) crystallization conditions PMID: 19388079

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  • 亚细胞定位：
    Membrane; Lipid-anchor. Cell membrane, sarcolemma. Cell membrane. Apical cell membrane. Cell projection, ruffle membrane. Cell projection, filopodium tip. Cell projection, growth cone. Cell projection, lamellipodium. Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, perinuclear region. Nucleus. Cell projection, neuron projection. Perikaryon.; [Isoform 2]: Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network.
  • 组织特异性：
    Ubiquitously expressed. Expressed in the epidermis, hair follicles and keratinocytes. Detected in platelets and in cell lines of megakaryocytic and erythrocytic lineages. Both isoform 1 and isoform 2 are detected in various cancer cell lines, with isoform
  • 数据库链接：

    HGNC: 16920

    OMIM: 602293

    KEGG: hsa:10519

    STRING: 9606.ENSP00000333873

    UniGene: Hs.715556