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  4. Recombinant Human Calcium/calmodulin-dependent protein kinase type II subunit gamma (CAMK2G)

Recombinant Human Calcium/calmodulin-dependent protein kinase type II subunit gamma (CAMK2G)

  • 货号：
    CSB-YP619775HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP619775HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP619775HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP619775HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP619775HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CAMK2G; CAMK; CAMK-II; CAMKGCalcium/calmodulin-dependent protein kinase type II subunit gamma; CaM kinase II subunit gamma; CaMK-II subunit gamma; EC 2.7.11.17
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-558
  • 氨基酸序列
    MATTATCTRF TDDYQLFEEL GKGAFSVVRR CVKKTSTQEY AAKIINTKKL SARDHQKLER EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIHQI LESVNHIHQH DIVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGEQQA WFGFAGTPGY LSPEVLRKDP YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT VTPEAKNLIN QMLTINPAKR ITADQALKHP WVCQRSTVAS MMHRQETVEC LRKFNARRKL KGAILTTMLV SRNFSAAKSL LNKKSDGGVK KRKSSSSVHL MPQSNNKNSL VSPAQEPAPL QTAMEPQTTV VHNATDGIKG STESCNTTTE DEDLKARSPE GRSSRDRTAP SAGMQPQPSL CSSAMRKQEI IKITEQLIEA INNGDFEAYT KICDPGLTSF EPEALGNLVE GMDFHKFYFE NLLSKNSKPI HTTILNPHVH VIGEDAACIA YIRLTQYIDG QGRPRTSQSE ETRVWHRRDG KWLNVHYHCS GAPAAPLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In the central nervous system, it is involved in the regulation of neurite formation and arborization. It may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.
  • 基因功能参考文献：
    1. point mutation R292P sufficient to disrupt gene expression and spatial learning PMID: 29934532
    2. The data suggest T287 autophosphorylation regulates substrate gating, an intrinsic property of the catalytic domain, which is amplified within the multivalent architecture of the calcium/calmodulin-dependent protein kinase II holoenzyme. PMID: 29391954
    3. These findings indicate that the CaMKII-mediated GluA1 phosphorylation of S567 and S831 is critical for P2X2-mediated AMPAR internalization and ATP-driven synaptic depression. PMID: 27624155
    4. Laminin is instructive and CaMKII is non-permissive for the formation of complex aggregates of acetylcholine receptors on myotubes in culture. PMID: 27964993
    5. A new molecular mechanism mediated by CAMK2gamma in intestinal epithelial cells during colitis-associated cancer (CAC) development, thereby providing a potential new therapeutic target for CAC. PMID: 28319059
    6. oxidative stress activated the TRPM2-CaMKII cascade to further induce intracellular ROS production, which led to mitochondria fragmentation and loss of mitochondrial membrane potential PMID: 28007458
    7. novel mechanism by which CAMK2gamma antagonizes mTORC1 activation during hepatocarcinogenesis PMID: 27819676
    8. Demonstrate that calcium/CaMKIIgamma/AKT signaling can regulate apoptosis and autophagy simultaneously in colorectal cancer cells. PMID: 26803057
    9. High CaMKIIgamma expression is associated with lung cancer. PMID: 25965829
    10. Dysfunction in CaMKII-based signaling has been linked with a host of cardiovascular phenotypes including heart failure and arrhythmia, and CaMKII levels are elevated in human and animal disease models of heart disease. PMID: 25577293
    11. Inhibition of CaMKII activity results in an upregulation of CaMKIV mRNA and protein in leukemia cell lines. PMID: 25446257
    12. CAMKIIgamma, HSP70 and HSP90 transcripts are differentially expressed in chronic myeloid leukemia cells from patients with resistant mutated disease. PMID: 24206096
    13. CaMKII-dependent microtube polymerization may be responsible for the enhanced uptake of PEI/ON complexes in A549 cells under oxidative stress conditions. PMID: 24634301
    14. Our data suggest that berbamine and its derivatives are promising agents to suppress liver cancer growth by targeting CAMKII PMID: 23960096
    15. CaMKII overexpression in mushroom body neurons increases activity dependent calcium responses. PMID: 23543616
    16. it can be concluded that CaMKII regulates the activity of ASIC1, which is associated with the ability of GBM cells to migrate. PMID: 24100685
    17. When inhibiting CaMKII, B-cell activating factor (BAFF) is attenuated and mediates protein phosphatase (PP)2A-Erk1/2 signaling and B-cell proliferation. PMID: 24269630
    18. the chronic gain-of-function defect in RyR2 due to CaMKII hyperphosphorylation is a novel mechanism that contributes to pathogenesis of type 2 diabetes PMID: 23516528
    19. results show that CAMKII and calmodulin contribute to IKK complex activation and thus to the induction of NF-kappaB in response to H. pylori infection PMID: 23463379
    20. Activated CaMK-II interacts with the C-terminal domain of diacylglycerol lipase-alpha (DGLalpha) and inhibits DGLalpha activity. PMID: 23502535
    21. Data indicate that CaMKII gamma as a specific and critical target of berbamine for its antileukemia activity. PMID: 23074277
    22. These findings revealed a fundamental role of CaMKII in the enteric nervous system. PMID: 22952977
    23. CaMKII T286A showed a mildly but significantly reduced rate of Ca(2+)/CaM-stimulated phosphorylation for two different peptide substrates (to ~75-84% of wild type). PMID: 22615928
    24. The CaMKII phosphorylation motif in the Nav1.5 DI-DII cytoplasmic loop is a critical point for proarrhythmic changes. PMID: 23008441
    25. CaMKII was also necessary for the phosphorylation of Raf-1 at S338 by serum, fibronectin and Ras. PMID: 22592532
    26. increases in Ca(2+) lead to CaMKII activation and subsequent Lck-dependent p66Shc phosphorylation on Serine 36. This event causes both mitochondrial dysfunction and impaired Ca(2+) homeostasis, which synergize in promoting Jurkat T-cell apoptosis. PMID: 21983898
    27. we review the cellular colocalization of CaMKII isoforms with special regard to the cell-type specificity of isoform expression in brain--REVIEW PMID: 22612808
    28. CaMKII binding to and phosphorylation of the NHE3 C terminus are parts of the physiologic regulation of NHE3 that occurs in fibroblasts as well as in the brush border of an intestinal Na(+)-absorptive cell. PMID: 22371496
    29. study demonstrates that premitotic condensation involves the activation of ClC-3 by Ca(2+)/calmodulin-dependent protein kinase II in glioma cells PMID: 22049206
    30. study shows CaMKII is recruited to the immunological synapse where it interacts with and phosphorylates Bcl10; propose a mechanism whereby Ca(2+) signals can be integrated at the immunological synapse through CaMKII-dependent phosphorylation of Bcl10 PMID: 21513986
    31. Ca(2+)/calmodulin-dependent kinase II participate in control of cell cycle progression and survival of irradiated CML cells. PMID: 21063097
    32. These results indicated that PP6 and CaMKII regulated apoptosis by controlling the expression level of p27. PMID: 20851109
    33. The interaction between CaMKII and its binding proteins was altered by the phosphorylation state of both the CaMKII and the partner. PMID: 20060891
    34. CaMKII is a molecular link translating intracellular calcium changes, which are intrinsically associated with glioma migration, to changes in ClC-3 conductance required for cell movement PMID: 20139089
    35. Our results suggest a first observation that CaMKII regulates TRAIL-mediated apoptosis of fibroblast-like synovial cells through Akt, standing an upstream of caspase-8-dependent cascades. PMID: 20149311
    36. Study further supports self-aggregation of CaMKII holoenzymes as the underlying mechanism that involves inter-holoenzyme T286-region/T-site interaction. PMID: 19840793
    37. cloning, genomic structure and detection of variants in subjects with Type II diabetes PMID: 12032636
    38. CaMKIIgamma is necessary to suppress MCAK depolymerase activity in vivo. PMID: 15775983
    39. A transgenic, constitutively active, Ca2+-independent form of CaMKgamma reduces positive selection of T cells by maintaining association of SHP-2 with the T cell receptor (TCR) complex, and halting TCR signaling. PMID: 16002660
    40. Amphetamine in a cell line induces a robust increase in cytosolic Ca2+ and concomitant activation of calcium/calmodulin-dependent protein kinase II (CaMKII). PMID: 17032905
    41. Significant cross-talk between calcium and retinoic acid signaling pathways regulates the differentiation of myeloid leukemia cells. PMID: 17431504
    42. insulin in the presence of Angiotensin II inhibits protein phosphatase-2A (PP-2A) and stimulates autonomous CaM kinase II activities and thus vascular smooth muscle migration PMID: 17553505
    43. IGF-II/mannose-6-phosphate receptor signaling induced cell hypertrophy and atrial natriuretic peptide/BNP expression via Galphaq interaction and protein kinase C-alpha/CaMKII activation in H9c2 cardiomyoblast cells. PMID: 18434368
    44. CaMKIIgamma is a critical regulator of multiple signaling networks regulating the proliferation of myeloid leukemia cells PMID: 18483256
    45. In Turner yndrome, loss of voltage-dependent inactivation is an upstream initiating event for arrhythmia phenotypes that are ultimately dependent on CaMKII activation. PMID: 19001023
    46. increased RyR2-dependent Ca2+ leakage due to enhanced CaMKII activity is an important downstream effect of CaMKII in individuals susceptible to AF induction. PMID: 19603549
    47. P2X7 receptor-triggered signalling pathways that regulate neurite formation in neuroblastoma cells. PMID: 19682070

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  • 亚细胞定位：
    Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 组织特异性：
    Expressed in skeletal muscle.
  • 数据库链接：

    HGNC: 1463

    OMIM: 602123

    KEGG: hsa:818

    STRING: 9606.ENSP00000319060

    UniGene: Hs.523045