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  4. Recombinant Human Carbonic anhydrase 12 (CA12), partial

Recombinant Human Carbonic anhydrase 12 (CA12), partial

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  • 中文名称：
    Recombinant Human Carbonic anhydrase 12(CA12),partial
  • 货号：
    CSB-YP004367HU
  • 规格：
    ￥1500
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP004367HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) CA12.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP004367HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) CA12.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    CA12
  • Uniprot No.：
  • 别名：
    CA 12; CA XII; CA-XII; CA12; CAH12_HUMAN; Carbonate dehydratase XII; Carbonic anhydrase 12; Carbonic anhydrase XII; Carbonic dehydratase; CAXII; FLJ20151; HsT18816; T18816; Tumor antigen HOM RCC 3.1.3; Tumor antigen HOM-RCC-3.1.3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Extracellular Domain
  • 来源：
    Yeast
  • 分子量：
    33.1kDa
  • 表达区域：
    25-301aa
  • 氨基酸序列
    APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The production of this Recombinant Human cry1Ac protein began at the genetic level, where the coding sequence for the cry1Ac protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (Yeast) so that the cells could be cultured and expressed the desired CA12 protein. And we finally got the recombinant CA12 protein with the purity of 90%+ determined by SDS-PAGE.

    The carbonic anhydrase 12 (CA12) gene encodes a zinc metalloenzyme that catalyzes reversible carbon dioxide hydration and thereby modulates a range of biological processes. CA12 is a type I membrane protein that has been found to be expressed in renal cell carcinoma and a range of other cancer types. There is some evidence that CA12 can promote tumor growth by driving micro environmental acidification. And some studies have primarily found that CA12 expression is restricted to certain cell types with maximal expression in terminally differentiated cells. In breast cancer patients, intratumoral CA12 upregulation is associated with better patient prognosis, whereas in oral cancer this gene is associated with tumor invasion and metastasis. Currently, CA12 is known to play central roles in regulating many cancers, but its function in the context of different cancers remains to be fully discussed.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Reversible hydration of carbon dioxide.
  • 基因功能参考文献：
    1. Our findings indicate that loss of CA XII function should be considered in individuals without CFTR mutations who exhibit CF-like features in the sweat gland and lung. PMID: 26911677
    2. The expression of HIF-1alpha, but not HIF-2alpha, decreased in degenerated disc samples and was positively correlated with carbonic anhydrase 12 expression. PMID: 26901836
    3. mutation CA12(E143K) causes mouth dryness disease due to aberrant CA12 glycosylation. PMID: 26486891
    4. Suggest CAXII as a new secondary marker of the multidrug resistance phenotype that influences Pgp activity directly. PMID: 25686827
    5. an upregulation of CAXII in human T-cell acute lymphoblastic leukemia samples supporting the case that CAXII may represent a new therapeutic target for T-ALL/LL. PMID: 23348702
    6. Serum CAXII levels were significantly higher in lung cancer patients than in healthy controls, providing evidence that CAXII may be a novel sero-diagnostic marker for lung cancer. PMID: 22439015
    7. The expression of CA XII in oral squamous cell carcinoma (OSCC) samples can predict the progression of OSCC and survival of OSCC patients. PMID: 22172588
    8. The development of acidosis with topiramate and zonisamide is not determined by drug dose or by treatment duration, but may be influenced by polymorphisms in the gene for CA type XII. PMID: 21278619
    9. Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12.Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12. PMID: 20132413
    10. Study identified the association of a Glu143Lys mutation in carbonic anhydrase 12 (CA12) with the disease. PMID: 21184099
    11. we demonstrate that the hyperchlohidrosis phenotype is due to a homozygous mutation in CA12, encoding carbonic anhydrase XII. PMID: 21035102
    12. CA12 may be used as a novel prognostic marker in combination with histologic grade of invasive cervical cancer. PMID: 21040567
    13. Carbonic anhydrase XII may affect the capability of invasion and migration of MDA-MB-231 cells, which may be mediated through the p38 MAPK pathway. PMID: 20434230
    14. Suggest that CA XII should be considered a potential prognostic and therapeutic target in medulloblastomas and supratentorial primitive neuroectodermal tumours. PMID: 20398423
    15. Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine. PMID: 20215053
    16. The non-pigmented ciliary epithelial cells from glaucoma eyes expressed higher levels of CAXII, which may be a targeted gene in glaucoma. PMID: 12676895
    17. CA XII showed no or weak immunoreaction in the normal gastric mucosa and was slightly increased in gastric tumors. PMID: 12854129
    18. The interplay between the functional von Hippel-Lindau tumor suppressor and CA IX/CA XII in colorectal tumors seems rather PMID: 15849821
    19. CA12 mRNA expression was detected in 88.1% of cervical cancers. PMID: 16416108
    20. CA XII is commonly expressed in diffuse astrocytomas and that it might be used as a biomarker of poor prognosis. PMID: 18322268
    21. Carbonic anhydrase (CA) IX (and possibly also CA XII) participates in pH regulation, which is important for survival of hypoxic cancer cells. Both enzymes are therefore promising targetable therapeutic molecules. [Review] PMID: 18336315
    22. Crucial role of ER in the regulation of the CA12 gene. PMID: 18451179
    23. In embryonic and early fetal tissues; there is no evidence of co-localization of CAIX and CAXII; CAIX and CAXII expression is closely related to cell origin and secretory activity involving proton transport, respectively. PMID: 19291313

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  • 相关疾病：
    Hyperchlorhidrosis, isolated (HCHLH)
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein. Cell membrane.
  • 蛋白家族：
    Alpha-carbonic anhydrase family
  • 组织特异性：
    Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat gl
  • 数据库链接：

    HGNC: 1371

    OMIM: 143860

    KEGG: hsa:771

    STRING: 9606.ENSP00000178638

    UniGene: Hs.210995