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  4. Recombinant Human Cell death activator CIDE-A (CIDEA)

Recombinant Human Cell death activator CIDE-A (CIDEA)

  • 货号：
    CSB-YP005431HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005431HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005431HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005431HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005431HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CIDEA
  • Uniprot No.：
  • 别名：
    CIDEACell death activator CIDE-A; Cell death-inducing DFFA-like effector A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-219
  • 氨基酸序列
    MEAARDYAGA LIRPLTFMGS QTKRVLFTPL MHPARPFRVS NHDRSSRRGV MASSLQELIS KTLDALVIAT GLVTLVLEED GTVVDTEEFF QTLGDNTHFM ILEKGQKWMP GSQHVPTCSP PKRSGIARVT FDLYRLNPKD FIGCLNVKAT MYEMYSVSYD IRCTGLKGLL RSLLRFLSYS AQVTGQFLIY LGTYMLRVLD DKEERPSLRS QAKGRFTCG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.
  • 基因功能参考文献：
    1. Cidea, unexpectedly, functions molecularly as an indirect inhibitor of thermogenesis via inhibition of UCP1 activity. PMID: 27923808
    2. These data establish a functional role for Cidea and suggest that, in humans, the association between Cidea levels in white fat and metabolic health is not only correlative but also causative. PMID: 26118629
    3. Since Cide-A protein plays a role in the development of metabolic diseases such as obesity, metabolic syndrome, type 2 diabetes and their vascular complications, CIDE -A gene and protein are potential therapeutic targets for these diseases. PMID: 24413203
    4. The lowest mean relative of the gene expression for CIDE-A was observed in the group of obese patients with aortic aneurysm and lipid disorders PMID: 25720106
    5. the association of tag-single nucleotide polymorphisms and haplotype structures of the CIDEA gene with obesity in a Han Chinese population, was investigated. PMID: 24057179
    6. CIDE proteins expression correlate with tumor and survival characteristics in patients with renal cell carcinoma. PMID: 23475172
    7. Insulin regulates CIDEA and CIDEC expression via PI3K, and it regulates expression of each protein via Akt1/2-and JNK2-dependent pathways, respectively, in human adipocytes. PMID: 21636835
    8. The proportion of subjects with CIDEA) gene V115F (G/T) polymorphism with phenotypes of metabolic syndrome in a Chinese population was significantly higher based on genotype, in the order: GG PMID: 21106268
    9. CIDEA binds to liver X receptors and regulates their activity in vitro. PMID: 21315073
    10. These data indicate that the carboxy-terminal domain of Cidea directs lipid droplet targeting, lipid droplet clustering, and triglyceride accumulation, whereas the amino terminal domain is required for Cidea-mediated development of enlarged lipid droplets PMID: 20810722
    11. CIDEC is essential for the differentiation of adipose tissue PMID: 20945533
    12. These results suggest that CIDEA and CIDEC are novel genes regulated by insulin in human adipocytes and may play key roles in the effects of insulin, such as anti-apoptosis and lipid droplet formation. PMID: 20154362
    13. Methylation status of CIDEA, HAAO and RXFP3 had significant association with microsatellite instability in endometrial tumors. PMID: 20211485
    14. We propose an important and human-specific role for CIDEA in lipolysis regulation and metabolic complications of obesity. PMID: 15919794
    15. results support a role for cell death-inducing DFFA (DNA fragmentation factor-alpha)-like effector A (CIDEA) alleles in human obesity PMID: 16186410
    16. deglycosylation of CIDE-A correlated with enhanced nuclear export of the protein, and that high level of nonglycosylated CIDE-A inhibited TGFbeta1-dependent cell death. PMID: 17080483
    17. No dysregulation of CIDEA expression was observed in individuals with the metabolic syndrome PMID: 17895319
    18. The expression of the CIDE-A gene was regulated by CpG methylation of the promoter region. PMID: 18033804
    19. F allele of the CIDEA gene may serve as a risk factor for phenotypes related to metabolic syndrome in Japanese men. PMID: 18328351
    20. Cidea and other lipid droplet proteins define a novel, highly regulated pathway of triglyceride deposition in human WAT. PMID: 18509062
    21. CIDEa is sequestered in mitochondria while transfer of this potentially dangerous protein from mitochondria into nucleus intensifies or even initiates apoptosis. PMID: 18645223
    22. CIDEA is involved in adipose tissue loss in cancer cachexia and this may, at least in part, be due to its ability to inactivate PDC, thereby switching substrate oxidation in human fat cells from glucose to FAs. PMID: 19010897

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  • 相关疾病：
    In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin-resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.
  • 亚细胞定位：
    Lipid droplet. Nucleus.
  • 组织特异性：
    Expressed in omental and subcutaneous adipose tissue (at protein level).
  • 数据库链接：

    HGNC: 1976

    OMIM: 604440

    KEGG: hsa:1149

    STRING: 9606.ENSP00000320209

    UniGene: Hs.249129