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  4. Recombinant Human Cell division cycle 7-related protein kinase (CDC7)

Recombinant Human Cell division cycle 7-related protein kinase (CDC7)

  • 货号：
    CSB-YP005023HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005023HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005023HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005023HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005023HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdc 7; CDC7; CDC7 cell division cycle 7; CDC7 cell division cycle 7 like 1; Cdc7 Kinase; CDC7 L1; Cdc7 like 1; CDC7 related kinase; CDC7-related kinase; CDC7_HUMAN; CDC7L 1; CDC7L1; Cell division cycle 7 homolog; Cell division cycle 7 kinase; Cell division cycle 7 like protein 1; Cell division cycle 7 related protein kinase; Cell division cycle 7-related protein kinase; HsCDC 7; HsCdc7; Hsk 1; Hsk1; HuCDC 7; huCdc7; MGC117361; MGC126237; MGC126238
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-574
  • 氨基酸序列
    MEASLGIQMD EPMAFSPQRD RFQAEGSLKK NEQNFKLAGV KKDIEKLYEA VPQLSNVFKI EDKIGEGTFS SVYLATAQLQ VGPEEKIALK HLIPTSHPIR IAAELQCLTV AGGQDNVMGV KYCFRKNDHV VIAMPYLEHE SFLDILNSLS FQEVREYMLN LFKALKRIHQ FGIVHRDVKP SNFLYNRRLK KYALVDFGLA QGTHDTKIEL LKFVQSEAQQ ERCSQNKSHI ITGNKIPLSG PVPKELDQQS TTKASVKRPY TNAQIQIKQG KDGKEGSVGL SVQRSVFGER NFNIHSSISH ESPAVKLMKQ SKTVDVLSRK LATKKKAIST KVMNSAVMRK TASSCPASLT CDCYATDKVC SICLSRRQQV APRAGTPGFR APEVLTKCPN QTTAIDMWSA GVIFLSLLSG RYPFYKASDD LTALAQIMTI RGSRETIQAA KTFGKSILCS KEVPAQDLRK LCERLRGMDS STPKLTSDIQ GHASHQPAIS EKTDHKASCL VQTPPGQYSG NSFKKGDSNS CEHCFDEYNT NLEGWNEVPD EAYDLLDKLL DLNPASRITA EEALLHPFFK DMSL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylate MCM2 and MCM3.
  • 基因功能参考文献：
    1. Higher expression of CDC7 in odontogenic keratocyst (OKC) and radicular cyst was shown in comparison to dentigerous cyst, while radicular cyst and OKC groups showed no difference in CDC7 expression. PMID: 30012245
    2. Results suggest a new role of Claspin in initiation of DNA replication during normal S phase through the recruitment of Cdc7 that facilitates phosphorylation of Mcm proteins. PMID: 27401717
    3. Increased Cdc7-dependent replication initiation is a hallmark of p53 gain-of function mutations in lung adenocarcinoma. PMID: 28887320
    4. Both CDC7 and DBF4 promoters bind E2F, suggesting that increased E2F activity in RB1 mutant cancers promotes increased DDK expression. Surprisingly, increased DDK expression levels are also correlated with both increased chemoresistance and genome-wide mutation frequencies. PMID: 28448802
    5. p53-dependent control of CDC7 levels is essential for blocking G1/S cell-cycle transition upon genotoxic stress. PMID: 27611229
    6. we propose that phosphorylation of TOP2A by CDC7/DBF4 in early S-phase prevents its localization and/or activity at centromeres, and inhibition of TOP2A function could be relevant to prevent premature separation of centromeric DNA. PMID: 27407105
    7. The data support a model where Cdc7 (de)phosphorylation is the molecular switch for the activation and inactivation of DNA replication in mitosis, directly connecting Cdc7 and PP1a/Cdk1 to the regulation of once-per-cell cycle DNA replication in mammalian cells. PMID: 27105124
    8. Our data show that Cdc7 is highly expressed in colorectal cancer PMID: 26208856
    9. The presence of the index SNP rs1192415 (TGFBR3-CDC7) was associated with visual field progression in POAG (primary open-angle glaucoma) patients. PMID: 26383992
    10. MiR-630 promoted apoptosis by downregulation of CDC7. PMID: 25255219
    11. The state of DUE-B phosphorylation is maintained by the equilibrium between Cdc7-dependent phosphorylation and PP2A-dependent dephosphorylation. PMID: 25258324
    12. The interaction between Claspin and Cdc7 is not dependent on Cdc7 kinase activity, but Claspin interaction with the DNA helicase subunit Mcm2 is lost upon Cdc7 inhibition. PMID: 23598722
    13. huCdc7 may play an important role in the development and progression of colorectal cancer. PMID: 23716994
    14. Cdcy is universally up-regulated in oral squamous cell carcinoma is an independent prognostic marker, contributing to resistance to DNA damaging agents. PMID: 23684929
    15. The results suggest that CDC7 expression level can be specific prognostic factors for Diffuse large B-cell lymphoma patients PMID: 22528513
    16. Significantly higher levels of apoptosis were detected in siCDC7-transfected cells. PMID: 22806309
    17. Cdc7 phosphorylates and interacts with Tob to inhibit the Cul4-DDB1(Cdt2)-dependent Tob degradation. PMID: 23066029
    18. cell death process induced by Cdc7 depletion PMID: 22574151
    19. bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. PMID: 21536671
    20. Inhibition of Cdc7 kinase activity in cancer cells restricts DNA replication and induces apoptotic cell death by an unprecedented molecular mechanism of action. PMID: 20647475
    21. Data syggest important implications for the continued development of promising Cdc7-targeted cancer therapies. PMID: 20707412
    22. cell division cycle 7 is a replication associated protein with relationships to gene amplification and genomic instability in breast carcinomas. PMID: 19896697
    23. Data show that CDC7L1 and CDC10 was up-regulated but the expression of CDK9, CDC20 and CLK3 was down- regulated in azoospermic testes. PMID: 19426592
    24. The interaction with LEDGF relieves autoinhibition of Cdc7-ASK kinase, imposed by the C terminus of ASK. PMID: 19864417
    25. Drf1, a nuclear cell cycle-regulated protein binds to Cdc7 and activates the kinase PMID: 12065429
    26. Down-regulation of Cdc7 by small interfering RNA in a variety of tumor cell lines causes an abortive S phase, leading to cell death by either p53-independent apoptosis or aberrant mitosis. PMID: 15466207
    27. CR (periphilin) retards S-phase progression by modifying expression of Cdc7 and other genes involved in progression of DNA replication PMID: 15474462
    28. ASKL1 in a complex with Cdc7 may play a role in normal progression of both S and M phases PMID: 15668232
    29. CINP is part of the Cdc7-dependent mechanism of origin firing and a functional and physical link between Cdk2 and Cdc7 complexes at the origins PMID: 16082200
    30. the majority of the Mcm2 isoforms phosphorylated by Cdc7 are not stably associated with chromatin PMID: 16446360
    31. Cdc7-Dbf4 kinase efficiently phosphorylates p150. PMID: 16826239
    32. Results describe the mapping of the sites in human Mcm2 protein that are phosphorylated by Cdc7. PMID: 16864800
    33. Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells. PMID: 16899510
    34. MCM4 phosphorylation by Cdc7 kinase facilitates its interaction with Cdc45 on chromatin PMID: 17046832
    35. Cdc7 kinase plays a role in maintaining cell viability during replication stress PMID: 17062569
    36. Cdc7/Dbf4 kinase activity inhibition affects specific phosphorylation sites on MCM2 in cancer cells PMID: 18286467
    37. the ATR-dependent activation of the p38 MAP kinase is a major signaling pathway that induces apoptotic cell death after depletion of Cdc7 in cancer cells PMID: 18625709
    38. increased Cdc7-Dbf4 abundance may be a common occurrence in human malignancies PMID: 18714392
    39. chromatin-bound CDT1 is first stabilized and subsequently displaced by CDC7 activity, thereby ensuring the timely execution of DNA replication PMID: 19054765
    40. These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling. PMID: 19111665
    41. differences in Cdc7 expression may account for some of the differences between malignant melanomas and benign melanocytic nevi. PMID: 19278428
    42. Increased Cdc7 protein levels were significantly associated with arrested tumor differentiation, advanced clinical stage, genomic instability, and accelerated cell cycle progression. PMID: 19318489
    43. Cdc7 phosphorylates Mcm2 promoting pre-replication complex assembly. PMID: 19647517

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, CDC7 subfamily
  • 数据库链接：

    HGNC: 1745

    OMIM: 603311

    KEGG: hsa:8317

    STRING: 9606.ENSP00000234626

    UniGene: Hs.533573