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  4. Recombinant Human Cellular nucleic acid-binding protein (CNBP)

Recombinant Human Cellular nucleic acid-binding protein (CNBP)

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  • 中文名称：
    Recombinant Human Cellular nucleic acid-binding protein(CNBP)
  • 货号：
    CSB-EP005637HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    CNBP
  • Uniprot No.：
  • 别名：
    CCHC type zinc finger nucleic acid binding protein; Cellular nucleic acid binding protein; Cellular nucleic acid-binding protein; CNBP; CNBP_HUMAN; CNBP1; DM2; Erythroid differentiation related; PROMM; Proximal myotonic myopathy nucleic acid binding protein; RNF163; Sterol regulatory element binding protein; ZCCHC22; Zinc finger protein 273; Zinc finger protein 9 (a cellular retroviral nucleic acid binding protein); Zinc finger protein 9; ZNF9
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein of Isoform 2
  • 来源：
    E.coli
  • 分子量：
    34.6kDa
  • 表达区域：
    2-170aa
  • 氨基酸序列
    SSNECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTIEATA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression. Has a role as transactivator of the Myc promoter. Binds single-stranded RNA in a sequence-specific manner.; Binds G-rich elements in target mRNA coding sequences. Prevents G-quadruplex structure formation in vitro, suggesting a role in supporting translation by resolving stable structures on mRNAs.; Binds to RNA.; Binds to RNA.; Binds to RNA.; Binds to RNA.; Binds to RNA.
  • 基因功能参考文献：
    1. The s report a previously unknown long noncoding RNA which, together with CNBP, is involved in the fine-tuned regulation of CCND1 mRNA stability, without which CCND1 exhibits, at most, partial expression. PMID: 29199958
    2. [CCTG]n repeat expansion, differently from the DM1 mutation, does not influence the methylation status of the CNBP gene and other molecular mechanisms are involved in the pathogenesis of Myotonic Dystrophy type 2.. PMID: 29291944
    3. RNA sequence preferences of unconventional RNA-binding proteins, Nudt21 and CNBP, has been described. PMID: 27956239
    4. A second point is that DM mutations, although located in noncoding regions, may reduce the expression of mutant alleles, raising questions whether loss-of-function may contribute to the phenotype, or possibly impose a safety limit on knockdown therapies that create or aggravate a DMPK or CNBP deficiency state PMID: 28376341
    5. CNBP is supporting translation by resolving stable structures on mRNAs. PMID: 28329689
    6. A G-rich motif in the lncRNA Braveheart interacts with Cnbp to specify the cardiovascular lineage. PMID: 27618485
    7. The cnbp overexpression rescued the Treacher Collins Syndrome phenotype in a dose-dependent manner by a reactive oxygen species-cytoprotective action that prevented the redox-responsive genes' upregulation but did not normalize the synthesis of ribosomal RNAs. PMID: 27711076
    8. High CNBP expression is associated with Medulloblastoma. PMID: 26460945
    9. CNBP overexpression caused increase of cell death and suppression of cell metastasis through its induction of G-quadruplex formation in the promoter of hnRNP K resulting in hnRNP K down-regulation PMID: 24594223
    10. Arginine methylation of CNBP in the RG motif does not change the subcellular localization but regulates its RNA binding activity. PMID: 24726729
    11. CNBP are novel antigens for SLE patients and the recognition of CNBP might be differentiated dependent on the level of arginine methylation. PMID: 23642268
    12. suggested a new possibility of CNBP as a potential anti-cancer target based on CNBP's biological function in c-myc transcription PMID: 23774591
    13. CNBP associates with the poly(A) binding protein and accumulates in stress granules. PMID: 23285195
    14. The co-segregation of Myotonic dystrophy type 2 with a recessive CLCN1 mutation provided the explanation for the unusual clinical findings for juvenile onset of myotonia in a 14-year-old female with Myotonic dystrophy type 2 and her affected mother PMID: 22407275
    15. Myotonic dystrophy 2(autosomal dominant, multisystem disorder caused by a CCTG tetranucleotide repeat expansion located in intron 1 of the zinc finger protein 9 gene (ZNF9 gene) on chromosome 3q 21.3.) described in Israeli Jewish European ancestry. PMID: 22332444
    16. CCTG repeat expansions in the CNBP gene are responsible for myotonic dystrophy type 2. PMID: 21204798
    17. Study concludes that DM2 patients from the Netherlands, including a North-African family, harbor a common haplotype surrounding the ZNF9 gene. PMID: 21224892
    18. These data suggest that Gis2 is functionally orthologous to ZNF9 and acts as a cap-independent translation factor. PMID: 21277287
    19. ZNF9 expression in myotonic dystrophy type 2 patients is altered at multiple levels. PMID: 20971734
    20. ZNF9 is abundantly expressed in human myofibres, where it is located in the sarcomeric I bands, and no modification of this pattern is observed in myotonic dystrophy type 2 muscles. PMID: 20102514
    21. Data identify ZNF9 as a regulator of cap-independent translation and indicate that ZNF9 activity may contribute mechanistically to the myotonic dystrophy type 2 phenotype. PMID: 20174632
    22. Six of seven of the Zn(2+) fingers from the CNBP protein can be used as substitutes for the Zn(2+) finger in the NH(2)-terminal position of HIV-1 nucleocapsid, and thus support virus replication PMID: 12857921
    23. The proximal myotonic myopathy phenotype is associated with DM2-(CCTG)(n) expansion mutations. PMID: 15261229
    24. In twenty-six individuals from a family with DM, the CCTG repeats in ZNF9 were found in normal range. PMID: 15476170
    25. results show that a single nucleotide polymorphism located in the first intron of the ZNF9 gene is in linkage disequilibrium with the DM2 mutation PMID: 15652222
    26. Results indicate that the (CCTG)n expansion in the ZNF9 intron does not appear to have a direct consequence on the expression of the gene itself. PMID: 16376058
    27. the downstream molecular effects of a DM2 mutation are triggered by the accumulation of CCUG repeat tract alone and do not decrease ZNF9 expression at the mRNA or protein level PMID: 16624843
    28. We present two first-degree relatives with an athletic clinical phenotype, pathological evidence of subsarcolemmal vacuolation, and molecular genetic confirmation of DM2(the molecular defect of the zinc finger protein 9 gene) PMID: 17068784
    29. PCBP2 and ZNF9 stimulate translation of the ornithine decarboxylase internal ribosomal entry site . PMID: 17327219
    30. These data contribute to the clinical and molecular correlation of ZNF9 gene short expansion in myotonic dystrophy. PMID: 18804219
    31. Validated occurrence of an unusual TG 3' splice site in intron 3. PMID: 17672918

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  • 相关疾病：
    Dystrophia myotonica 2 (DM2)
  • 亚细胞定位：
    Nucleus. Cytoplasm. Endoplasmic reticulum.; [Isoform 1]: Cytoplasm.; [Isoform 2]: Cytoplasm.; [Isoform 4]: Cytoplasm.; [Isoform 5]: Cytoplasm.; [Isoform 6]: Cytoplasm.; [Isoform 8]: Cytoplasm.
  • 组织特异性：
    Expressed in the liver, kidney, spleen, testis, lung, muscle and adrenal glands.
  • 数据库链接：

    HGNC: 13164

    OMIM: 116955

    KEGG: hsa:7555

    STRING: 9606.ENSP00000410769

    UniGene: Hs.518249