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  4. Recombinant Human Centromere/kinetochore protein zw10 homolog (ZW10)

Recombinant Human Centromere/kinetochore protein zw10 homolog (ZW10)

  • 货号：
    CSB-YP027154HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP027154HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP027154HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP027154HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP027154HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ZW10
  • Uniprot No.：
  • 别名：
    Centromere/kinetochore protein zw10; Centromere/kinetochore protein zw10 homolog; HZW 10; HZW10; Kinetochore associated homolog; KNTC1AP; MGC149821; Zeste white 10; Zeste white 10 homolog; ZW 10; ZW 10 homolog centromere/kinetochore protein; ZW 10 kinetochore associated homolog; ZW10 (Drosophila) homolog centromere/kinetochore protein; Zw10; ZW10 homolog centromere/kinetochore protein (Drosophila); ZW10 homolog centromere/kinetochore protein; ZW10 kinetochore associated homolog; ZW10, kinetochore associated, homolog (Drosophila); ZW10_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-779
  • 氨基酸序列
    ASFVTEVLA HSGRLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL PSMQSAQGLI TQVDKLSEDI DLLKSRIESE VRRDLHVSTG EFTDLKQQLE RDSVVLSLLK QLQEFSTAIE EYNCALTEKK YVTGAQRLEE AQKCLKLLKS RKCFDLKILK SLSMELTIQK QNILYHLGEE WQKLIVWKFP PSKDTSSLES YLQTELHLYT EQSHKEEKTP MPPISSVLLA FSVLGELHSK LKSFGQMLLK YILRPLASCP SLHAVIESQP NIVIIRFESI MTNLEYPSPS EVFTKIRLVL EVLQKQLLDL PLDTDLENEK TSTVPLAEML GDMIWEDLSE CLIKNCLVYS IPTNSSKLQQ YEEIIQSTEE FENALKEMRF LKGDTTDLLK YARNINSHFA NKKCQDVIVA ARNLMTSEIH NTVKIIPDSK INVPELPTPD EDNKLEVQKV SNTQYHEVMN LEPENTLDQH SFSLPTCRIS ESVKKLMELA YQTLLEATTS SDQCAVQLFY SVRNIFHLFH DVVPTYHKEN LQKLPQLAAI HHNNCMYIAH HLLTLGHQFR LRLAPILCDG TATFVDLVPG FRRLGTECFL AQMRAQKGEL LERLSSARNF SNMDDEENYS AASKAVRQVL HQLKRLGIVW QDVLPVNIYC KAMGTLLNTA ISEVIGKITA LEDISTEDGD RLYSLCKTVM DEGPQVFAPL SEESKNKKYQ EEVPVYVPKW MPFKELMMML QASLQEIGDR WADGKGPLAA AFSSSEVKAL IRALFQNTER RAAALAKIK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER.
  • 基因功能参考文献：
    1. The RZZ complex was purified to homogeneity and subjected to systemic crystallization. PMID: 25849506
    2. hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain PMID: 21345172
    3. Overexpression, microinjection and knockdown experiments revealed that ZW10 is involved in membrane trafficking between the endoplasmic reticulum and Golgi PMID: 15029241
    4. show that ZW10 interacting protein-1(Zwint-1) is required and is sufficient for kinetochore localization of Zeste White 10 (ZW10) in HeLa cells PMID: 15485811
    5. ZW10 serves as a general regulator of dynein function throughout the cell cycle. PMID: 16505164
    6. RINT-1 coordinates the localization and function of ZW10 by serving as a link between ZW10 and the SNARE complex comprising syntaxin 18. PMID: 16571679
    7. targeting of ZW10 to the perinuclear Golgi region was found to depend on the perinuclear accumulation of dynactin PMID: 17560939
    8. RNAi-mediated depletion of ZW10 markedly-reduced congression efficiency. PMID: 17680027
    9. Rab6 regulates distinct Golgi trafficking pathways involving two separate protein complexes: ZW10/RINT-1 and COG. PMID: 17699596
    10. Accumulation of ZW10 at tensionless kinetochores stems from a 4-fold reduction of kinetochore turnover rate and requires aurora B kinase activity. PMID: 18065224
    11. Stable hZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with hZwint-1, and is essential for mitotic checkpoint arrest. PMID: 18268100
    12. The interaction between ZW10 and dynamitin showed that the N-terminal region of ZW10 is the major binding site for dynamitin and, like full-length ZW10, could move along microtubules to the centrosomal area in a dynein-dynactin-dependent manner. PMID: 18782227
    13. Results together suggest that NAG links between p31 and ZW10-RINT-1 and is involved in Golgi-to-ER transport. PMID: 19369418

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  • 亚细胞定位：
    Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Lipid droplet.
  • 蛋白家族：
    ZW10 family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 13194

    OMIM: 603954

    KEGG: hsa:9183

    STRING: 9606.ENSP00000200135

    UniGene: Hs.503886