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  4. Recombinant Human Centromere protein H (CENPH)

Recombinant Human Centromere protein H (CENPH)

  • 货号：
    CSB-EP005211HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    CENPH
  • Uniprot No.：
  • 别名：
    CENP H; CENP-H; CENPH; CENPH_HUMAN; Centromere protein H; ICEN35; Interphase centromere complex protein 35; Kinetochore protein CENP H; NNF1; NNF1; MIND kinetochore complex component; homolog; PMF1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    55.5kDa
  • 表达区域：
    1-247aa
  • 氨基酸序列
    MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLLRLRAQTKQQLLEYKSMVDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKKLALDRMRLSTALKKNLEKISRQSSVLMDNMKHLLELNKLIMKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEIKITTVIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVDMM
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
  • 基因功能参考文献：
    1. In summary, CENP-H may be involved in cell proliferation and apoptosis of hepatocellular carcinoma (HCC)cells through the mitochondrial apoptotic pathway. Combined with previous studies, the data provide a new perspective on HCC development and progression. PMID: 28498417
    2. Upregulation of centromere protein H is associated with progression of renal cell carcinoma PMID: 26248586
    3. propose that CSPP1 cooperates with CENP-H on kinetochores to serve as a novel regulator of kinetochore microtubule dynamics for accurate chromosome segregation PMID: 26378239
    4. CENP-H was overexpressed in HCC, and its level of upregulation was an independent prognostic indicator, suggesting that CENP-H may be an effective therapeutic strategy for the treatment of HCC. PMID: 23970101
    5. CENP-H promotes the proliferation of human gastric cancer cells PMID: 22381774
    6. High expression of CENP-H in gastric cancer indicates poor prognosis and Survivin may mediate its procancer role. PMID: 22999412
    7. Breast cancer patients with high CENP-H expression had short overall survival. CENP-H expression was related with clinical stage. PMID: 21880184
    8. upregulated CENP-H and Ki67 levels are significantly associated with short relapse-free survival in hypopharyngeal squamous cell carcinoma (HSSC); these factors may be predictors of a relapsing phenotype in HSSC cases PMID: 22631655
    9. Sp1 and Sp3 bind to the CENPH minimal promoter and function as a regulator of the transcription of CENPH in nasopharyngeal carcinomas. PMID: 22682030
    10. CENP-H is up-regulated and plays an important role in the aneuploidy frequently observed in colorectal cancers. PMID: 15930286
    11. The CENP-H-I complex may function, in part, as a marker directing CENP-A deposition to centromeres. PMID: 16622420
    12. has an important impact on the architecture and function of the human kinetochore complex PMID: 16875666
    13. CENP-H has a role in preventing progression of nasopharyngeal carcinoma PMID: 17255272
    14. CENP-C and CENP-H co-localize to the CENP-A chromatin domain. PMID: 17651496
    15. CENP-H protein has a role in esophageal carcinoma progression PMID: 18700042
    16. expression of CENPH was much higher in cancer cell lines & lung cancer tissue than normal cells & adjacent noncancerous lung tissues; results show that high CENPH protein expression was related to poor outcome in patients with nonsmall cell lung cancer PMID: 19170237
    17. TRIM36 has a ubiquitin ligase activity and interacts with centromere protein-H. PMID: 19232519
    18. Upregulation of CENP-H in tongue cancer is associated with disease progression. PMID: 19500341
    19. CENP-H-containing complex facilitates deposition of newly synthesized CENP-A into centromeric chromatin in cooperation with FACT and CHD1. PMID: 19625449

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC during both interphase and metaphase.
  • 蛋白家族：
    CENP-H/MCM16 family
  • 数据库链接：

    HGNC: 17268

    OMIM: 605607

    KEGG: hsa:64946

    STRING: 9606.ENSP00000283006

    UniGene: Hs.631967