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  4. Recombinant Human Centromere protein T (CENPT)

Recombinant Human Centromere protein T (CENPT)

  • 货号：
    CSB-YP005221HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005221HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005221HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005221HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005221HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CENPT
  • Uniprot No.：
  • 别名：
    C16orf56 ; CENP T ; CENP-T; Cenpt; CENPT_HUMAN; Centromere protein T; Chromosome 16 open reading frame 56; ICEN22 ; Interphase centromere complex protein 22
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-561
  • 氨基酸序列
    MADHNPDSDS TPRTLLRRVL DTADPRTPRR PRSARAGARR ALLETASPRK LSGQTRTIAR GRSHGARSVG RSAHIQASGH LEEQTPRTLL KNILLTAPES SILMPESVVK PVPAPQAVQP SRQESSCGSL ELQLPELEPP TTLAPGLLAP GRRKQRLRLS VFQQGVDQGL SLSQEPQGNA DASSLTRSLN LTFATPLQPQ SVQRPGLARR PPARRAVDVG AFLRDLRDTS LAPPNIVLED TQPFSQPMVG SPNVYHSLPC TPHTGAEDAE QAAGRKTQSS GPGLQKNSPG KPAQFLAGEA EEVNAFALGF LSTSSGVSGE DEVEPLHDGV EEAEKKMEEE GVSVSEMEAT GAQGPSRVEE AEGHTEVTEA EGSQGTAEAD GPGASSGDED ASGRAASPES ASSTPESLQA RRHHQFLEPA PAPGAAVLSS EPAEPLLVRH PPRPRTTGPR PRQDPHKAGL SHYVKLFSFY AKMPMERKAL EMVEKCLDKY FQHLCDDLEV FAAHAGRKTV KPEDLELLMR RQGLVTDQVS LHVLVERHLP LEYRQLLIPC AYSGNSVFPA Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
  • 基因功能参考文献：
    1. Levels of centromere aberrations increase upon depletion of CENP-A, CENP-C, and CENP-T/W, during replicative senescence, and in cancer cells. PMID: 28167779
    2. report a novel disease gene encoding the constitutive inner kinetochore member CENPT, which is involved in kinetochore targeting and assembly, resulting in severe growth failure in two siblings of a consanguineous family PMID: 29228025
    3. Upon cross-linking, the entire CENPA/CENPB/CENPC/CENPT complex is nuclease-protected over an alpha-satellite dimer that comprises the fundamental unit of centromeric chromatin. We conclude that CENPA/CENPC and CENPT pathways for kinetochore assembly are physically integrated over young alpha-satellite dimers. PMID: 27384170
    4. FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres. PMID: 27284163
    5. We used a synthetic system to dissect how CenH3(CENP-A) contributes to the accumulation of CENP-C and CENP-T, two key components that are necessary for the formation of functional kinetochores PMID: 25843710
    6. CENP-T directly interacts with Ndc80, which in turn promotes KNL1/Mis12 complex recruitment at kinetochores during chromosome segregation. PMID: 25660545
    7. CSN5 regulates the stability of the inner kinetochore components CENP-T and CENP-W, providing the first direct link between CSN5 and the mitotic apparatus, highlighting the role of CSN5 as a multifunctional cell cycle regulator. PMID: 23926101
    8. CENP-T provides a structural platform for outer kinetochore assembly. PMID: 23334297
    9. results reveal the molecular function of CENP-T proteins and demonstrate how the Ndc80 complex is anchored to centromeres in a manner that couples chromosome movement to spindle dynamics PMID: 22561346
    10. The CENP-T/W complex assembles through a dynamic exchange mechanism in late S-phase and G2, is required for mitosis in each cell cycle and does not persist across cell generations. PMID: 21695110
    11. Study demonstrates that CENP-W forms a DNA-binding complex together with the CCAN component CENP-T; this complex directly associates with nucleosomal DNA and with canonical histone H3, but not with CENP-A, in centromeric regions. PMID: 19070575
    12. CENP-T exchange into centromeres is restricted to the S-phase of the cell cycle as revealed by FRAP, suggesting a coreplicational loading mechanism. PMID: 19412974

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    CENP-T/CNN1 family
  • 数据库链接：

    HGNC: 25787

    OMIM: 611510

    KEGG: hsa:80152

    STRING: 9606.ENSP00000219172

    UniGene: Hs.288382