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  4. Recombinant Human Centromere protein W (CENPW)

Recombinant Human Centromere protein W (CENPW)

  • 货号：
    CSB-YP701863HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP701863HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP701863HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP701863HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP701863HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CENPW
  • Uniprot No.：
  • 别名：
    C6ORF173; cancer upregulated gene 2; Cancer-up-regulated gene 2 protein; CENP-W; cenpw; CENPW_HUMAN; Centromere protein W; chromosome 6 open reading frame 173; CUG2; hypothetical protein LOC387103
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-88
  • 氨基酸序列
    MALSTIVSQR KQIKRKAPRG FLKRVFKRKK PQLRLEKSGD LLVHLNCLLF VHRLAEESRT NACASKCRVI NKEHVLAAAK VILKKSRG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
  • 基因功能参考文献：
    1. we suggest that STAT1HDAC4 signaling induces malignant tumor features such as EMT and sphere formation in CUG2overexpressing cancer cells. PMID: 30226605
    2. Report provides evidence that CUG2 induces the EMT in human lung cancer cells via enhancement of TGF-beta signaling and that that TGF-beta signaling is a potential target for CUG2-mediated oncogenesis. PMID: 27974707
    3. It has been proposed that CENP-W may function as a booster of beta-TrCP1 nuclear import to increase the oncogenicity of beta-TrCP1. PMID: 27861801
    4. FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres. PMID: 27284163
    5. CENP-W interacts with hnRNPU and may contribute to kinetochore-microtubule attachment in mitotic cells. PMID: 26881882
    6. CENP-W is a novel kinetochore component that may be involved in the EZH2-mediated silencing machinery. PMID: 26111449
    7. We propose that CENP-W, by influencing proper kinetochore assembly, particularly microtubule docking sites, can confer spindle pole resistance to traction forces exerted by motor proteins during chromosome congression PMID: 25329824
    8. CUG2-mediated antiviral activity can be reduced by ISG15 deficiency in A549 cells, which eventually enhances sensitivity of the cells to vesicular stomatitis virus infection. PMID: 24452380
    9. our results suggest that CUG2 enhances metastasis and drug resistance through STAT1 activation, which eventually contributes to tumor progression PMID: 23917355
    10. CSN5 regulates the stability of the inner kinetochore components CENP-T and CENP-W, providing the first direct link between CSN5 and the mitotic apparatus, highlighting the role of CSN5 as a multifunctional cell cycle regulator. PMID: 23926101
    11. we propose that VSV treatment combined with the selective regulation of genes such as STAT1 and OASL2 will improve therapeutic outcomes for CUG2-overexpressing tumors. PMID: 23306614
    12. Single nucleotide polymorphism in CENPW is associated with the length of reproductive lifespan. PMID: 22131368
    13. B23 may function in the assembly of the kinetochore complex by interacting with CENP-W during interphase PMID: 22002061
    14. The CENP-T/W complex assembles through a dynamic exchange mechanism in late S-phase and G2, is required for mitosis in each cell cycle and does not persist across cell generations. PMID: 21695110
    15. Sp1 together with Sp3 may function as the main regulator of the basal and serum-induced transcription of CENP-W. PMID: 20180024
    16. Overexpression of CUG2 induces apoptosis in SKOV-3 cells. PMID: 20648695
    17. Observational study of gene-disease association. (HuGE Navigator) PMID: 20846217
    18. Observational study of gene-disease association. (HuGE Navigator) PMID: 20546612
    19. data indicate that activation of p38 MAPK and Ras has critical roles in reoviral replication in CUG2-expressing tumor cells. PMID: 20075984
    20. These findings suggest CUG2 is a novel tumor-associated gene that is commonly activated in various human cancers and exhibits high transforming activities; it possibly belongs to a transcription regulator family that is involved in tumor biogenesis. PMID: 17610844
    21. Study demonstrates that CENP-W forms a DNA-binding complex together with the CCAN component CENP-T; this complex directly associates with nucleosomal DNA and with canonical histone H3, but not with CENP-A, in centromeric regions. PMID: 19070575
    22. CUG2 is a new component of the human centromeric complex that is required for proper chromosome segregation during mitosis. PMID: 19533040
    23. Observational study of gene-disease association. (HuGE Navigator) PMID: 19266077

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Nucleus matrix. Nucleus, nucleolus.
  • 蛋白家族：
    CENP-W/WIP1 family
  • 组织特异性：
    Highly expressed in ovary, liver, lung and pancreas and to a lower extent in breast and gastrointestinal tract cancers; such as those of the colon, rectum and stomach. Overexpressed in high grade breast invasive tumors. Expressed in many cancer cell types
  • 数据库链接：

    HGNC: 21488

    OMIM: 611264

    KEGG: hsa:387103

    STRING: 9606.ENSP00000357311

    UniGene: Hs.486401