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  4. Recombinant Human Chloride channel protein ClC-Ka (CLCNKA), partial

Recombinant Human Chloride channel protein ClC-Ka (CLCNKA), partial

  • 中文名称：
    Recombinant Human Chloride channel protein ClC-Ka(CLCNKA),partial,Yeast
  • 货号：
    CSB-YP005487HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Chloride channel protein ClC-Ka(CLCNKA),partial,Yeast
  • 货号：
    CSB-EP005487HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Chloride channel protein ClC-Ka(CLCNKA),partial,Yeast
  • 货号：
    CSB-EP005487HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Chloride channel protein ClC-Ka(CLCNKA),partial,Yeast
  • 货号：
    CSB-BP005487HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Chloride channel protein ClC-Ka(CLCNKA),partial,Yeast
  • 货号：
    CSB-MP005487HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CLCNKA
  • Uniprot No.：
  • 别名：
    CLCNKAChloride channel protein ClC-Ka; Chloride channel Ka; ClC-K1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.
  • 基因功能参考文献：
    1. Single loci of tag Single Nucleotide Polymorphisms of CLCNKA_B are not enough to increase the Essential Hypertension susceptibility, the combination of CLCNKA SNP, salt, marine products, meat, edible oil consumption is associated with elevated risk. PMID: 25919862
    2. R8W and G47R, two naturally occurring barttin mutations identified in patients with Bartter syndrome type IV, reduce barttin palmitoylation and CLC-K/barttin channel activity. PMID: 26013830
    3. HEK293 cells the potentiating effect of niflumic acid (NFA) on CLC-Ka/barttin and CLC-Kb/barttin channels seems to be absent while the blocking efficacy of niflumic acid and benzofuran derivatives observed in oocytes is preserved PMID: 24863058
    4. following variables were significantly associated with an estimated glomerular filtration rate: age, type 2 diabetes, total cholesterol, LDL-cholesterol, and the CLCNKA GG genotype PMID: 23850580
    5. The variant CLCNKA risk allele, telegraphed by linked variants in the adjacent HSPB7 gene, uncovers a previously overlooked genetic mechanism affecting the cardio-renal axis. PMID: 21248228
    6. Identify a protein region that is involved in calcium binding and that is likely undergoing conformational changes underlying the complex gating of CLC-K channels. PMID: 20805576
    7. Combined impairment of ClC-Ka and ClC-Kb results in phenotype that mimics neonatal Barrter's syndrome with deafness PMID: 15044642
    8. Barttin modulates trafficking and function of ClC-K1 and ClC-Kb channels PMID: 16849430
    9. CLCNKA genetic variants may have roles in salt-sensitive hypertension PMID: 17510212
    10. The structure of the cytoplasmic domain of CLCNKA reveals a conserved interaction interface. PMID: 17562318
    11. Disruption of the gene encoding Barttin, BSND, results in a 'double knockout' of the functions of both ClCKA and ClCKB, manifesting as Bartter syndrome type IV with sensorineural deafness and an especially severe salt-losing phenotype. PMID: 18094726
    12. ClC-Ka and ClC-Kb differ in how conformational changes are translated to the extracellular domain, despite the fact that the cytoplasmic domains share the same quaternary structure PMID: 18648499

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  • 相关疾病：
    Bartter syndrome 4B, neonatal, with sensorineural deafness (BARTS4B)
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Chloride channel (TC 2.A.49) family, CLCNKA subfamily
  • 组织特异性：
    Expressed predominantly in the kidney. All nephron segments expressing BSND also express CLCNK proteins.
  • 数据库链接：

    HGNC: 2026

    OMIM: 602024

    KEGG: hsa:1187

    STRING: 9606.ENSP00000332771

    UniGene: Hs.591533