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  4. Recombinant Human Cholinesterase (BCHE)

Recombinant Human Cholinesterase (BCHE)

  • 货号：
    CSB-YP002604HU
  • 规格：
    ￥1500
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Acylcholine acylhydrolase; BCHE; Butyrylcholine esterase; CHE1; CHE2; CHLE_HUMAN; Choline esterase II; Cholinesterase (serum) 2; Cholinesterase 1; Cholinesterase; E1; Pseudocholinesterase; Pseudocholinesterase E1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    67.1kDa
  • 表达区域：
    29-602aa
  • 氨基酸序列
    EDDIIIATKNGKVRGMNLTVFGGTVTAFLGIPYAQPPLGRLRFKKPQSLTKWSDIWNATKYANSCCQNIDQSFPGFHGSEMWNPNTDLSEDCLYLNVWIPAPKPKNATVLIWIYGGGFQTGTSSLHVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEAPGNMGLFDQQLALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPGSHSLFTRAILQSGSFNAPWAVTSLYEARNRTLNLAKLTGCSRENETEIIKCLRNKDPQEILLNEAFVVPYGTPLSVNFGPTVDGDFLTDMPDILLELGQFKKTQILVGVNKDEGTAFLVYGAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGKESILFHYTDWVDDQRPENYREALGDVVGDYNFICPALEFTKKFSEWGNNAFFYYFEHRSSKLPWPEWMGVMHGYEIEFVFGLPLERRDNYTKAEEILSRSIVKRWANFAKYGNPNETQNNSTSWPVFKSTEQKYLTLNTESTRIMTKLRAQQCRFWTSFFPKVLEMTGNIDEAEWEWKAGFHRWNNYMMDWKNQFNDYTSKKESCVGL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    To produce recombinant Human BCHE protein, a well-established recombinant DNA technology is the key. A DNA template of BCHE was constructed with N-terminal 6xHis tag using the technique. Once the template was made, the recombinant Human BCHE protein could be produced with it efficiently. CUSABIO has built a strict QC system to ensure quality. The expression region is 29-602aa of the Human BCHE. The purity of this recombinant is 90% determined by SDS-PAGE.

    BCHE is a protein coding gene that encodes Cholinesterase (Butyrylcholine esterase). According to some studies, BCHE may have the following features.
    The cloned BCHE gene maps to a single chromosome site, 3q26. GWAS of butyrylcholinesterase activity identified four novel loci, independent effects within BCHE and secondary associations with metabolic risk factors. Potent AChE and BChE inhibitors were isolated from seeds of Peganum harmala Linn by bioassay-guided fractionation. Neuroprotective tricyclic and tetracyclic BChE inhibitors release reversible inhibitors after carbamate transfer. Variation at locus 116 of the BCHE gene associating with enzyme activity and body mass index. BCHE, through its K variant, but not a nearby marker, is a susceptibility factor for AD and enhances AD risk defined only by APOE蓻4 in an age-dependent manner.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
  • 基因功能参考文献：
    1. An increased frequency of the BChE K-allele in multiple sclerosis patients as compared to controls was found. PMID: 29358722
    2. This study validated preoperative serum BChE levels as an independent prognostic factor for urinary tract urothelial carcinoma after radical nephroureterectomy. PMID: 29177431
    3. we present and examine means of manipulating brain BChE levels by viral gene transfer, either regionally or globally, to modulate ghrelin signaling for long-term therapeutic purposes and to set the stage for exploring the neurophysiological impact of such an intervention. PMID: 28698452
    4. The BChE-K polymorphisms are associated with deleterious changes in cognitive decline in MCI patients treated with donepezil for 3 years. PMID: 27911294
    5. BCHE-K* positive subjects (and APOE-epsilon4) display an earlier age of onset of Alzheimer's Disease and an accelerated cognitive decline. PMID: 27567841
    6. Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base.() PMID: 28544359
    7. HuBChE sequesters OP nerve agent in the bloodstream preventing the nerve agent from reaching critical target organ systems. HuBChE was effective when used as both a pre-treatment and as a post-exposure therapy. PMID: 28225154
    8. Significance of BChE Genetic Variants to Risk of Toxicity from Cholinesterase Inhibitors (review) PMID: 27551784
    9. Age, sex or smoking status did not influence butyrylcholinesterase activity in a healthy population. PMID: 28465191
    10. Mutations in the butyrylcholinesterase gene were associated with prolonged effect of succinylcholine or mivacurium. PMID: 27031121
    11. prolonged apnea after suxamethonium came about as a result of butyrylcholinesterase deficiency or mutation or acquired conditions leading to a decrease in the plasma cholinesterase activity PMID: 26439437
    12. the molecular mechanisms by which point mutations may lead to silent BChE variant or alter catalytic activity, is reported. PMID: 27062896
    13. BChE expression might be regulated by alpha-linolenic acid in HepG2 cells. PMID: 27106529
    14. Nine out of 18 (50%) individuals with butyrylcholinesterase activity below 2000 U/L had a mutation in 5'UTR (32G/A), intron 2 (c.1518-121T/C) or exon 4 (c.1699G/A; the K variant mutation). PMID: 27109752
    15. No significant difference in BChE levels of healthy pregnant women and high-risk pregnant women who had undergone a cesarean section under general anesthesia. PMID: 26955768
    16. Association between polymorphisms of BCHE and vitiligo in a two-step genetic association study. Upon confirmation of genetic association, the effect was investigated by a genotype-phenotype correlation experiment. PMID: 26189613
    17. Low serum Butyrylcholinesterase levels are associated with recurrence in prostate cancer. PMID: 26223693
    18. BCHE-K is associated with a reduced risk for Alzheimer disease and Lewy body dementia whereas APOEvarepsilon4 is associated with more rapid cognitive decline. PMID: 26757188
    19. Low BChE activity as a predictor of mortality in acute myocardial infarction might be related to its association with poor cardiac function. PMID: 27468571
    20. rs1803274 polymorphism of the BCHE gene represents a risk factor for in-stent restenosis after percutaneous coronary intervention. PMID: 26497592
    21. Complement component C3 and butyrylcholinesterase activity are associated with neurodegeneration and clinical disability in multiple sclerosis. PMID: 25835709
    22. BChE is a strong predictor for cardiac mortality specifically in younger patients with acute coronary syndrome aged between 45 and 64 years. PMID: 25933219
    23. High resolution melting analysis for the butyrylcholinesterase atypical variant genotyping is a simple, rapid, sensitive and low cost method PMID: 25336127
    24. There is a link between systemic inflammatory markers, oxidative stress, the PON192rs662-Q allele and BuChE-non-UU-phenotype and their corresponding enzymatic activity which may be considered as a risk factor for the severity of rheumatoid arthritis. PMID: 25179377
    25. There is a significant decrease in serum ChE activity after severe trauma PMID: 25471831
    26. This study proves that the p.Ala34Val BChE variant determines the "silent" phenotype (unable to hydrolyze succinylcholine and mivacurium). PMID: 25264279
    27. Data indicate that butyrylcholinesterase (BChE) was significantly associated with overall survival (OS). PMID: 24951323
    28. analysis of the T920C mutation allele frequency in butyrylcholinesterase gene in an Indian population PMID: 25447891
    29. Plasma arylesterase activity is positively associated with plasma ChE specific activity in a nested case-control study. PMID: 24473115
    30. BChE-K carriers are protected from the pathological detriments of Alzheimer's disease that affect cortical thickness. PMID: 24479631
    31. results confirm the association of APOE with Abeta deposition and represent the largest known effect of BCHE on an AD-related phenotype. PMID: 23419831
    32. Results indicate that BuChE non-UU phenotype and PON 55M allele are significant risk factors for systemic lupus erythematosus. PMID: 24399815
    33. Our findings suggest that the AA genotype of rs1803274 is a risk factor for crack cocaine use, which is more addictive than powder cocaine use. PMID: 24312228
    34. We report the case of a 65-year-old patient who was diagnosed with butyrylcholinesterase resulting in prolonged paralysis and extended mechanical ventilation PMID: 24446003
    35. Two mutants of human BCHE can effectively eliminate (-)-cocaine, cocaethylene and norcocaine in simplified kinetic models of cocaine abuse and overdose associated with the concurrent use of cocaine and alcohol. PMID: 24870023
    36. BChE activity might be involved in the pathogenesis of preeclampsia through influence on lipid and lipoprotein metabolism and oxidative stress. PMID: 23650977
    37. The present data are still inconclusive with respect to a possible contribution of common genetic variants in BCHE to the pathogenesis of attention-deficit/hyperactivity syndrome. PMID: 24041656
    38. CocH3 (BCHE mutant) with an identical concentration with that of the endogenous BChE in human plasma can effectively eliminate both cocaine and norcocaine in a simplified kinetic model of cocaine abuse. PMID: 24125115
    39. BCHE 1914G allele showed higher frequency in the obese group. Carriers of 1914G BCHE allele showed lower mean BChE activity.BCHE 1914G allele is influencing physiological mechanisms related to obesity. PMID: 24001779
    40. Data indicate that high AChE affinity of the compounds was achieved by optimizing different substituents on the pyridazinone ring, without sacrificing the AChE/BuChE selectivity profile. PMID: 23466605
    41. Water in the active site gorge of the D70G mutant is more easily depleted than that in wild-type BChE. PMID: 23782236
    42. These findings show that BCHE can hydrolyze 2-Arachidonoylglycerol which may be evidence of a more specific role for BCHE in endocannabinoid regulation. PMID: 23689009
    43. This review examines the roles of gender and BuChE genotype in the phenotypic expression of Alzheimer disease[review] PMID: 22402324
    44. Elevated levels of BuChE observed in active multiple sclerosis (MS) lesions could be related to the decompaction of myelin characteristic of the disorder. PMID: 22778864
    45. [review] Butyrylcholinesterase has diverged both structurally and in terms of tissue and cellular expression patterns from acetylcholinesterase. PMID: 22750491
    46. aspirin is hydrolyzed in plasma by two enzymes, BChE and a new extracellular form of platelet-activating factor acetylhydrolase, PAFAH1b2. PMID: 23508960
    47. characterized the catalytic activities of wild-type BChE and the A199S/F227A/S287G/A328 W/Y332G mutant against both (+)- and (-)-cocaine at the same time under the same experimental conditions PMID: 22917637
    48. verify amplification and/or deletion in the ACHE, BCHE, EPHB4 and MME genes in 32 samples of sporadic breast cancer PMID: 23063927
    49. performed molecular dynamics simulation to probe the structural stability of Indian variant (L307P) in comparison with wild and other BChE variants (D70G, E497V, V142M) having differential esterase activity PMID: 23123771
    50. Alkylating molecules were synthesized and the crystal structures they form with soman-aged butyrylcholinesterase were solved. PMID: 22922115

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  • 相关疾病：
    Butyrylcholinesterase deficiency (BChE deficiency)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Type-B carboxylesterase/lipase family
  • 组织特异性：
    Detected in blood plasma (at protein level). Present in most cells except erythrocytes.
  • 数据库链接：

    HGNC: 983

    OMIM: 177400

    KEGG: hsa:590

    STRING: 9606.ENSP00000264381

    UniGene: Hs.420483