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  4. Recombinant Human Chymase (CMA1)

Recombinant Human Chymase (CMA1)

  • 货号：
    CSB-YP005599HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005599HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005599HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005599HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Alpha-chymase; Chymase 1; Chymase 1 mast cell; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; Chymase; Chymase; heart; Chymase; mast cell; CMA1; CMA1_HUMAN; CYH; CYM; EC 3.4.21.39; Mast cell chymase 1; Mast cell protease 3; Mast cell protease 5; Mast cell protease I; Mast cell protease III; Mcp-5; MCP3P; Mcpt5; MCT1; MGC119890; MGC119891; MMCP-5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-247
  • 氨基酸序列
    IIGGTECKP HSRPYMAYLE IVTSNGPSKF CGGFLIRRNF VLTAAHCAGR SITVTLGAHN ITEEEDTWQK LEVIKQFRHP KYNTSTLHHD IMLLKLKEKA SLTLAVGTLP FPSQFNFVPP GRMCRVAGWG RTGVLKPGSD TLQEVKLRLM DPQACSHFRD FDHNLQLCVG NPRKTKSAFK GDSGGPLLCA GVAQGIVSYG RSDAKPPAVF TRISHYRPWI NQILQAN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
  • 基因功能参考文献：
    1. These findings reveal the critical role of hypoxia in producing local angiotensin II by a lactate-chymase-dependent mechanism and highlight the importance of local angiotensin II in regulating radioresistance of hypoxic tumor cells. PMID: 28205588
    2. Cleavage of the alarmins by Human mast cell chymase and human neutrophil cathepsin G suggests a function in regulating excessive inflammation. PMID: 28053237
    3. activation of endothelial CLP/chymase may directly relate to the increased inflammatory phenotypic changes in the vascular system in women with preeclampsia PMID: 19494363
    4. Placenta-derived chymotrypsin-like protease contributes to the altered endothelial barrier function in preeclampsia. PMID: 19126871
    5. promoter single nucleotide polymorphism is not associated with Dengue Hemorrhagic Fever and Dengue Shock Syndrome in Vietnam and Philippines PMID: 25797204
    6. Our data demonstrated that mast cell chymase plays an important role in keloid formation through TGF-beta1/Smad signaling pathway. PMID: 25120737
    7. These data suggest a possible contribution of human chymase activation to LPS-induced mortality PMID: 24516344
    8. we present data indicating that MC tryptase and chymase contribute to the development of OSF and malignant transformation of the overlying epithelium. PMID: 24874976
    9. The polymorphisms and haplotypes of the CMA1 locus are associated with cardiac hypertrophy in male patients with symptomatic aortic stenosis. PMID: 24823657
    10. CMA1 gene single nucleotide polymorphisms is associated with essential hypertension. PMID: 25119908
    11. Significant association between the AG genotype of CMA1 A polymorphism and Angina Pectoris and Ventricular Extrasystoles was observed. PMID: 25102745
    12. The accumulated data in this review suggest that mast cells, their tryptases, and their chymases play important roles in tissue repair. PMID: 24507159
    13. demonstrate the generation of Pso p27 from SCCA1 with extracts from psoriatic scale and even more remarkably, the generation of Pso p27 from SCCA1 in the presence of mast cell associated chymase PMID: 24560885
    14. The present results indicated PRCP rs7104980 can be considered as a marker for EH and Hap3 GAGCACTAACA (PRCP) and Hap16 TTTA (CMA1) might be associated with essential hypertension in Chinese Han population. PMID: 22679278
    15. Purified chymase mixed with fragmented heparin derived from heparanase-expressing cells show greater release from collagen gels than the enzyme alone or mixed with macromolecular heparin derived from mock cells. PMID: 24344642
    16. Data indicate that the best Fynomer (the binding proteins derived from the Fyn SH3 domain) was found to bind chymase with a KD of 0.9 nM and koff of 6.6x10 (-4) s (-1) , and to selectively inhibit chymase activity with an IC 50 value of 2 nM. PMID: 22653218
    17. Both IgE and chymase associate with diabetes status. PMID: 22194960
    18. As mast cells are an important source of VEGF, tryptase, and chymase, these findings suggest that mast cell activation and mast cell-derived mediators participate in the development of Dengue hemorrhagic fever. PMID: 22363824
    19. Chymase rs1800875 polymorphism is associated with the progression of immunoglobulin A (IgA) nephropathy in Korean patients. PMID: 21150220
    20. a dominant role of cardiac chymase in the formation of Ang II from Ang-(1-12) PMID: 22180785
    21. Two different populations of mast cells were found in melanocytic skin; one expressing both chymase and tryptase and the other with tryptase only. PMID: 22102069
    22. mast cell-derived protein is present in uterine cervical carcinoma PMID: 21274549
    23. Polymorphism rs1800875 of CMA1 may be associated with serum IgE level in coronary heart disease subjects, but not with chymase level in normal coronary intimae PMID: 21796807
    24. human chymase contributes to blood glucose levels and mortality during the progression of diabetes PMID: 21786536
    25. short tandem repeat genetic polymorphism is associated with bronchial asthma in a Swiss cohort study PMID: 20736038
    26. Elevated maternal chymase activity and enhanced protease immunostaining in the maternal vessel endothelium may constitute the exacerbated inflammatory state and account for the increased vascular Ang II sensitivity in preeclampsia. PMID: 20670150
    27. Positions 143 (Arg) and 192 (Lys) in human mast cell chymase contribute to the strong preference for negatively charged amino acids at substrate position P2'. PMID: 20423454
    28. The levels of tryptase and chymase expression are greatly increased in human lung tissue of anaphylactic shock. PMID: 19697770
    29. The S1 primary specificity pocket defines substrate specificity of chymases. PMID: 11852067
    30. The mast cell chymase A3255 allele was shown to have an effect on HDL cholesterol metabolism. PMID: 12047032
    31. The local release of mast cell chymase has potentially profound effects on airway smooth muscle cell function by disruption of the cell-associated matrix and inhibition of epidermal growth factor-induced smooth muscle cell proliferation. PMID: 12097409
    32. Results suggest the additive effect of angiotensin I-converting enzyme (ACE) and heart chymase (CMA) gene polymorphisms on the increase in left ventricular mass in NIDDM patients. PMID: 12165749
    33. Chymase may play a role in heart remodeling by increasing Ang II formation and activating MMP-9, and the regulation of collagen I gene expression. PMID: 12359984
    34. Both the ACE and chymase-like enzyme activities in the aneurysmal aortae were significantly higher than those in the control aortae. PMID: 12484503
    35. Degradation of phospholipid transfer protein (PLTP) and PLTP-generated pre-beta-high density lipoprotein by this enzyme in mast cells impairs high affinity efflux of cholesterol from macrophage foam cells. PMID: 12531890
    36. Interactions among the loops bordering and defining the active site appear to influence both the zymogen and the activated conformations of chymase in this model. PMID: 12614156
    37. albumin is a substrate of human chymase PMID: 12815038
    38. new class of chymase inhibitor through a substituent analysis of MWP00965 chemically synthesized PMID: 14592513
    39. chymase depletes pre-beta-high density lipoprotein, which impairs ATP-binding cassette transporter A1- but not scavenger receptor class B type I-mediated lipid efflux to high density lipoprotein PMID: 14701812
    40. MCT1 immunoreactivity was visible in blood vessel walls as early as the 13th week of gestation mainly in the visual cortical plate and subplate. PMID: 14757520
    41. mast cell chymase-1 is unlikely to influence blood pressure levels in the Japanese population. PMID: 15106801
    42. Bladder fibrosis may be mediated by mast cell chymase stimulation of collagen synthesis. PMID: 15227657
    43. The synthesis of new potential inhibitors of human chymase is described PMID: 15449728
    44. Tryptase and chymase and protein levels were determined in mast cells in fibrosarcoma. PMID: 15638376
    45. mast cell chymase activates ERK and p38 probably through G-protein-coupled receptor, and the ERK but not p38 cascade may have a crucial role in chymase-induced migration of eosinophils PMID: 15919053
    46. A novel (TG)n(GA)m repeat polymorphism 254 bp downstream of the mast cell chymase (CMA1) gene is associated with atopic asthma and total serum IgE levels. PMID: 15924217
    47. Chymase-induced apoptosis of conjunctival epithelial cells represents anoikis, which is a slowly occurring apoptotic process induced by lack of adhesion to an extracellular matrix. PMID: 16020275
    48. Significant association between the CMA1 promoter polymorphism rs1800875 and atopic eczema supports the hypothesis that CMA1 serves as candidate gene for atopic eczema. PMID: 16134991
    49. activated human skin mast cells (MCs) convert CTAP-III into biologically active NAP-2 through proteolytic cleavage by released chymase. PMID: 16317101
    50. The CMA1 polymorphisms studied do not contribute to disease susceptibility in Japanese or Dutch sarcoidosis patients. PMID: 16446531

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  • 亚细胞定位：
    Secreted. Cytoplasmic granule. Note=Mast cell granules.
  • 蛋白家族：
    Peptidase S1 family, Granzyme subfamily
  • 组织特异性：
    Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
  • 数据库链接：

    HGNC: 2097

    OMIM: 118938

    KEGG: hsa:1215

    STRING: 9606.ENSP00000250378

    UniGene: Hs.135626