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  4. Recombinant Human Claudin-5 (CLDN5), partial

Recombinant Human Claudin-5 (CLDN5), partial

  • 货号：
    CSB-YP005507HU1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005507HU1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005507HU1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005507HU1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005507HU1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CLDN5
  • Uniprot No.：
  • 别名：
    CLDN5; AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 基因功能参考文献：
    1. Results show that Claudin 5 expression is activated by SIRT1 deacetylating and potentiating KLF4. PMID: 28888043
    2. the present study revealed the distinct expression profiles of claudin5, 7 and 8 in nonneoplastic mucosal tissues and gastric carcinoma tissues. Furthermore, the expression of these claudin proteins was highly associated with metastatic progression and prognosis in patients with gastric carcinoma PMID: 29901188
    3. High CLND5 expression is associated with brain metastasis. PMID: 29328410
    4. Claudin-5 regulates blood-brain barrier permeability by modifying brain microvascular endothelial cell proliferation, migration. PMID: 28961379
    5. miR-30a-5p inhibits proliferation, metastasis, and EMT, and upregulates the expression of tight junction claudin-5 in UTUC cells. PMID: 28829370
    6. Claudin-5 was shown to be regulated VEGFR2/PI3K-Akt dependently by VEGF and PI3K-Akt independently by histamine. Interleukin-8 was shown to downregulate claudin-5 by histamine. PMID: 27873103
    7. This study shown the changes in gene expression of CDH5 and CLDN5 due to shear stress within individual differentiations also revealed no trend. PMID: 28774343
    8. Claudin-5 single-nucleotide polymorphism (SNP) rs885985 has two major alleles, G and A, which encode for glutamine (Q) or a stop signal, respectively, resulting in distinct overlapping open readings. PMID: 28445614
    9. Data show that the charge of Lys65 in claudin 1 (Cldn1) and Glu158 in claudin 3 (Cldn3), and of Gln57 in claudin 5 (Cldn5) are necessary for tight junction (TJ) strand formation. PMID: 28415153
    10. suggest that the reduction of CLDN5, 7, and 18 expression loses the suppressive ability of interaction between PDK1 and Akt and causes sustained phosphorylation of Akt, resulting in the disordered proliferation in lung squamous carcinoma cells PMID: 27884700
    11. The levels of ESM1, CLDN5, IL-1beta, IL-6, and TNF-alpha were significantly higher in the migraine attack group than in the control group PMID: 27010153
    12. analysis of the membrane driven cis interactions of claudin-5 proteins in the formation of the blood brain barrier tight junctions PMID: 26654362
    13. the expression of claudin-5 and claudin-9 was down-regulated while the expression of claudin-8 was up-regulated in cervical carcinoma tissues compared with adjacent non-neoplastic tissues. PMID: 26464708
    14. Plasma CLDN5 levels decreased in patients with stable asthma compared with those in control subjects, suggesting that asthma therapy can decrease plasma CLDN5 levels PMID: 26409663
    15. These data support that loss of claudin-5 in cardiomyocytes and endothelial cells is prevalent in human heart failure PMID: 25440958
    16. the relationship between vWF and claudin-5, which are indicators of endothelial cell dysfunction and tight junction activity, may be a predictor of disease activity in rheumatoid arthritis. PMID: 25973092
    17. The findings suggest that down regulated CLDN1 and CLDN5 genes have potential relevance in relation to the progression of glioblastoma multiforme. PMID: 25345514
    18. Claudin-5 overexpression promoted the interactions of claudin-1 and claudin-2 and enhanced the barrier function of retinal cells. PMID: 25323998
    19. Data show that C-terminal domain of Clostridium perfringens enterotoxin (cCPE)-binding to claudin 1 and claudin 5 is prevented by two residues in extracellular loop 2 of Cld1 (Asn(150) and Thr(153)) and Cld5 (Asp(149) and Thr(151)). PMID: 25342221
    20. The present study demonstrates that high expression of claudins 1, 4, 5 and down-regulation of claudin-7 are positive prognostic markers and are associated with good outcome and increased survival rates. PMID: 24815833
    21. loss of claudin 5 expression in the tumour vasculature points to a leaky vessel type PMID: 24519061
    22. The results of this study showed weak association of the CLDN5 locus with schizophrenia in Chinese. PMID: 20452046
    23. folding and assembly of CLDN3 and CLDN5 into the tight junction are controlled by non-conserved residues in the transmembrane 3 and extracellular loop 2 segments PMID: 24478310
    24. silencing of claudin-5 did significantly attenuate simvastatin-mediated endothelial cell barrier protection in response to thrombin PMID: 24028293
    25. VE-cadherin and claudin-5 were studied in nasal polyp microvessels. PMID: 23474739
    26. Matrix metalloproteinase-9 leads to claudin-5 degradation via the NF-kappaB pathway in BALB/c mice with eosinophilic meningoencephalitis caused by Angiostrongylus cantonensis. PMID: 23505411
    27. Chorionic gonadotropin induces VEGFA-dependent downregulation of claudin 5, which increases the endothelial permeability in the coculture system. PMID: 23465821
    28. Over-expression of claudin-5 was sufficient to prevent replication-deficient virus-induced permeability PMID: 23115643
    29. Claudin-5 expression and junctional organization controls intercellular barriers of human dermal microvascular but not human umbilical vein endothelial cells. PMID: 23288152
    30. Study portrays a role for Claudin-5 in cell motility involving the N-WASP signalling cascade indicating a possible role for Claudin-5 in the metastasis of human breast cancer. PMID: 22559840
    31. Letter: confirm the endothelial cell origin of cutaneous angiosarcoma and demonstrate that both endoglin and claudin-5 show promise as markers for cutaneous angiosarcoma. PMID: 23000880
    32. CLDN5 gene DNA meyhylation suggesting the pathogenesis of idiopathic pulmonary fibrosis. PMID: 22700861
    33. study demonstrated that claudin 5 is a protein with a short half-life that can undergo polyubiquitinationmainly on lysine 199, which apparently leads to its subsequent degradation PMID: 22389112
    34. this study portrays a new and interesting role for Claudin-5 in cell motility involving the N-WASP and ROCK signalling cascade PMID: 22038628
    35. GDNF increased the expression of claudin-5 and the transendothelial electrical resistance (TEER) of BMECs and PnMECs, whereas BDNF did not have this effect PMID: 22002662
    36. Galphai2 as a novel claudin-5 partner required for TJ integrity in brain endothelial cells. PMID: 22333621
    37. ERG plays a pivotal role in regulating endothelial cell barrier function and that this effect is mediated in part through its regulation of CLDN5 gene expression. PMID: 22235125
    38. Claudin-5 is a promising new marker for angiosarcomas and hemangioendotheliomas. PMID: 21959309
    39. Interendothelial claudin-5 expression depends on cerebral endothelial cell-matrix adhesion by beta(1)-integrins. PMID: 21772312
    40. This study sought to determine the cell signaling mechanism controlling endothelial CLDN5 expression during acute lung injury. PMID: 20525806
    41. tyrosine phosphorylation of claudin-5 is involved in the increased paracellular permeability of central nervous system-derived vascular endothelium induced by TGF-beta1. PMID: 21168935
    42. Claudin-1, -3, -4, -5, and -7 are expressed in developing human lung from week 12 to week 40 with distinct locations and in divergent quantities. PMID: 20478039
    43. Increased claudin-5 expression is associated with aggressive behavior in serous ovarian adenocarcinoma. PMID: 20044634
    44. Claudin 5 is transiently expressed during the development of the retinal pigment epithelium. PMID: 11944085
    45. The Blood-Brain Barrier against small molecules (<800 D), but not larger molecules, was selectively affected in Claudin-5 deficient mice. PMID: 12743111
    46. Airway tight junctions are regulated by claudin interactions that confer the selectivity of the junction. PMID: 12909588
    47. Showed that residues located C-terminal to the last transmembrane domain of claudin 5 are required for the proper targeting to apical TJ.s. PMID: 15260435
    48. CLDN5 protein may predispose to schizophrenia PMID: 15363474
    49. CLDN5 may be involved in forming a permeability barrier in the gut and, together with DQB, confer a susceptibility to schizophrenia. PMID: 15617864
    50. CLDN5 may be associated with schizophrenia in Chinese of Han descent PMID: 15820333

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  • 亚细胞定位：
    Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Claudin family
  • 数据库链接：

    HGNC: 2047

    OMIM: 602101

    KEGG: hsa:7122

    STRING: 9606.ENSP00000384554

    UniGene: Hs.505337