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  4. Recombinant Human Cleavage and polyadenylation specificity factor subunit 5 (NUDT21)

Recombinant Human Cleavage and polyadenylation specificity factor subunit 5 (NUDT21)

  • 货号：
    CSB-YP016170HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016170HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016170HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016170HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016170HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NUDT21
  • Uniprot No.：
  • 别名：
    CFIM 25; CFIM25; Cleavage and polyadenylation specific factor 5 25 kD subunit; Cleavage and polyadenylation specific factor 5 25 kDa; Cleavage and polyadenylation specific factor 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; Cleavage and polyadenylation specificity factor subunit 5; Cleavage factor Im complex 25 kDa subunit; CPSF 25; CPSF 25 kDa subunit; CPSF 5; CPSF25; CPSF5; CPSF5_HUMAN; DKFZp686H1588; Nucleoside diphosphate linked moiety X motif 21; Nucleoside diphosphate-linked moiety X motif 21; Nudix (nucleoside diphosphate linked moiety X) type motif 21; Nudix hydrolase 21; Nudix motif 21; NUDT 21; Nudt21; Pre mRNA cleavage factor Im (25kD); Pre mRNA cleavage factor Im 25 kDa subunit; Pre mRNA cleavage factor Im 25kD subunit; Pre-mRNA cleavage factor Im 25 kDa subunit
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-227
  • 氨基酸序列
    SVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides.
  • 基因功能参考文献：
    1. Findings suggest that CFIm25 plays an important role in lung cancer cell proliferation through regulating the alternative polyadenylation (APA) of oncogenes. PMID: 29928883
    2. RNA sequence preferences of unconventional RNA-binding proteins, Nudt21 and CNBP, has been described. PMID: 27956239
    3. Loss of NUDT21 shortened the 3'-UTR of various oncogenes in hepatocellular carcinoma cells. The shorter 3'-UTR contained less miRNA binding sites, which enabled the oncogenes to evade miRNA regulation and become overexpressed in HCC, leading to unregulated cancer cell proliferation. PMID: 28964783
    4. it was demonstrated that the CFIm25 protein was also downregulated in osteosarcoma tissues, and inhibited the proliferation and promoted the apoptosis of the cells. Elucidating the roles of miR181a and CFIm25 in osteosarcoma not only assists in further understanding the pathogenesis and progression of this disease, but also offers novel targets for effective therapies. PMID: 27633853
    5. The s identify NUDT21 as a novel candidate for intellectual disability and neuropsychiatric disease, and elucidate a mechanism of pathogenesis by MeCP2 dysregulation via altered alternative polyadenylation. PMID: 26312503
    6. Structure of a CFI(m)25/CFI(m)68 RRM heterotetramer and biochemical data indicated that CFIm25 specifically recognized two UGUA elements, CFIm68 facilitates looping of the intervening RNA. CFIm-mediated RNA looping may regulate alternative polyadnylation. PMID: 21295486
    7. Crystal structure of CFIm25-CFIm68 RRM heterotetramer illustrated that CFIm25 homodimer is clamped by two CFIm68 monomers on each side of the dimer interface. PMID: 21295486
    8. Data provide evidence that CFIm exists as a heterotetramer of 25-kD, 59-kD and 68-kD subunits of CFIm: CFIm25, CFIm59 and CFIm68. PMID: 20695905
    9. Crystallographic and biochemical experiments suggest that CF I(m)25 specifically recognizes UGUA RNA sequence and binds to two UGUA simultaneously PMID: 20479262
    10. crystal structures of the CFI(m)25 homodimer in complex with UGUAAA and UUGUAU RNA sequences PMID: 20479262
    11. region in the subunit of CF I(m) involved in RNA binding, protein-protein interactions, and subcellular localization PMID: 15169763
    12. Evidence that CFIm25 regulates alternative poly(A) site selection of genes with tandem poly(A) signals in their 3'-UTRs. PMID: 17098938
    13. Results from crystallographic and biochemical experiments suggest that CF I(m)25 makes use of its Nudix fold to bind but not hydrolyze ATP and diadenosine tetraphosphate. PMID: 18445629
    14. The crystal structure of human CPSF5 was solved at 1.9 A resolution. PMID: 18767156

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Nudix hydrolase family, CPSF5 subfamily
  • 组织特异性：
    Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 数据库链接：

    HGNC: 13870

    OMIM: 604978

    KEGG: hsa:11051

    STRING: 9606.ENSP00000300291

    UniGene: Hs.528834