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  4. Recombinant Human Cyclin-dependent kinase 4 inhibitor B (CDKN2B)

Recombinant Human Cyclin-dependent kinase 4 inhibitor B (CDKN2B)

  • 货号：
    CSB-YP005092HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005092HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005092HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005092HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CDK inhibitory protein; CDK4B Inhibitor; Cdkn2b; CDN2B_HUMAN; Cyclin dependent kinase 4 inhibitor B; Cyclin Dependent Kinase Inhibitor 2B; Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4; Cyclin dependent kinases 4 and 6 binding protein; Cyclin-dependent kinase 4 inhibitor B; INK4B; MTS 2; MTS-2; MTS2; Multiple tumor suppressor 2; Multiple Tumor Supressor 2; OTTHUMP00000021154; OTTHUMP00000021155; p14 CDK inhibitor; p14 INK4b; p14-INK4b; P15; p15 CDK inhibitor; p15 inhibits CDK4; p15 INK4b; p15-INK4b; p15INK4B; TP 15; TP15
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-138
  • 氨基酸序列
    MREENKGMPS GGGSDEGLAS AAARGLVEKV RQLLEAGADP NGVNRFGRRA IQVMMMGSAR VAELLLLHGA EPNCADPATL TRPVHDAARE GFLDTLVVLH RAGARLDVRD AWGRLPVDLA EERGHRDVAG YLRTATGD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
  • 基因功能参考文献：
    1. Concurrent mutations, in genes such as CDKN2B or RB1, were associated with worse clinical outcome in lung adenocarcinoma patients with EGFR active mutations. PMID: 29343775
    2. CDKN2B expression in HPDE6-C7 human pancreatic ductal cells is induced by oncogenic KRAS via TGF-beta signaling. PMID: 28892048
    3. findings suggest that the GG genotype of the CDKN2B-AS1 gene variant rs4977574, which has been previously associated with an increased CAD risk, is also associated with a decreased susceptibility to the development of hypertension PMID: 29791233
    4. CDKN2B gene locus is a cardiovascular risk in ethnic Saudi Arabs PMID: 29894795
    5. ANRIL plays an important role in the formation of atherosclerosis, and the artificial modification of ANRIL transcripts should be considered following the development of this disease. (Review) PMID: 29270737
    6. CDKN2A/B locus SNPs may impact T2D risk by modulating islet gene expression and beta-cell proliferation. PMID: 29432124
    7. Complete methylation and loss of p15 gene expression causes susceptibility to relapse and decreased survival in APL patients. Thus, p15 promoter hypermethylation is a prospective prognostic indicator and a reliable clinical aid in assessment of patients with APL. PMID: 28052659
    8. Patients with higher levels of DNA methylation of CDKN2B may bear increased risk for aortic arch calcification PMID: 27773886
    9. E2F1 induces TINCR transcriptional activity and accelerates gastric cancer progression via activation of TINCR/STAU1/CDKN2B signaling axis. PMID: 28569791
    10. ANRIL may reduce p15INK4B expression through inhibiting TGF-beta/Smad signaling pathway, promoting invasion and metastasis of TC cells, and the silencing of ANRIL inhibits the invasion and metastasis of TPC-1 cells PMID: 27507052
    11. The obtained data suggested a negative association between copy number variation of the CDK2B genes and Atherothrombotic stroke. PMID: 28477716
    12. Among 8 SNPs in 3 loci that showed at least nominal association (P < 5.00E-02) in the primary cohort, a representative SNP for each loci (rs2157719 for CDKN2B-AS1, rs33912345 for SIX6, and rs9913911 for GAS7) were selected PMID: 29261660
    13. Results show that expression of CDKN2B was reduced significantly in colorectal cancer (CRC) and its promotor is targeted by miR-18b which controls its regulation. PMID: 28784723
    14. Mechanistic investigations demonstrated that BLACAT1 had a key role in G1/G0 arrest, and showed that BLACAT1 can repress p15 expression by binding to EZH2, thus contributing to the regulation of CRC cell cycle and proliferation. Our results suggest that BLACAT1, as a cell cycle regulator, may serve as a potential target for colon cancer prevention and treatment in human CRC PMID: 28277544
    15. DNA methylation of CDKN2B may play a potential role in artery calcification. PMID: 27905995
    16. These data support the hypothesis that decreased p15 expression is a robust biomarker for distinguishing nevus from melanoma. PMID: 27855847
    17. Total 1208 differentially expressed genes were screened, and 5 coronary artery disease (CAD)-associated miRNAs (including miR-92a) were predicted associated with CAD. The SVM classifier was constructed based on the 41 featured genes and had high recognition efficiency. Only one lncRNA CDKN2B-antisense targeting miR-92a was obtained. PMID: 28760552
    18. Upregulation of ZEB1-AS1 colorectal cancer promotes cell proliferation and inhibits cell apoptosis. In addition, cell cycle inhibitory protein p15 participates in the oncogenic function of ZEB1-AS1. Collectively, ZEB1-AS1 has asignificant effect on colorectal cancer pathological process and serves as a valuable prognostic biomarker for colorectal cancer. PMID: 28618933
    19. Genetic association analyses using the cpRNFLT with 768 points suggest that the CDKN2B gene was associated with paracentral/lower hemifield scotomas. PMID: 28472212
    20. We found that CDKN2B was a virtual target of miR-15a-5p with potential binding sites in the 3'UTR of CDKN2B (77-83 bp). We also showed that miR-15a-5p could bind to the CDKN2B 3'UTR. The data revealed a negative regulatory role of miR-15a-5p in the apoptosis of smooth muscle cells via targeting CDKN2B, and showed that miR-15a-5p could be a novel therapeutic target of AAA. PMID: 28214350
    21. Meta-analysis indicates that CDKN2B gene rs1063192 polymorphism is significantly associated with a decreased risk of glaucoma. [meta-analysis] PMID: 28416752
    22. results suggest that the cyclin dependent kinase inhibitor 2B rs10811656 locus might be associated with coronary artery disease in a Chinese Han population PMID: 27507036
    23. Polymorphism rs1063192 in CDKN2B is not a risk factor for Open-Angle Glaucoma in Saudi cohort. PMID: 27541204
    24. The CC genotype of CDKN2B rs1063192 in the hsa-miR-323b-5p binding site increased the risk of gestational diabetes mellitus in pregnant Chinese Han women PMID: 25990668
    25. Genetic risk variants in the CDKN2B gene is associated with somatic biomarkers in glioma. PMID: 26839018
    26. Aberrant methylation of the promoter regions of the p15INK4b gene in patients with acute myeloid leukemias. PMID: 27459612
    27. Inhibited HO-1 expression attenuated the hypermethylation of CDKN2B by suppressing DNMT1, which was conducive to treatment by cooperating with decitabine. PMID: 26782424
    28. Tumor suppressor ASXL1 is essential for the activation of INK4B expression in response to oncogene activity and anti-proliferative signals PMID: 26470845
    29. This study investigated the potential roles of CDKN2B-related piRNA in leukemia cells to provide a potential tumorigenesis model of leukemia PMID: 26205624
    30. CDKN2B Loss Promotes Progression from Benign Melanocytic Nevus to Melanoma PMID: 26183406
    31. Children with ALL have higher levels of p15 CpG island methylation than a control group of children with ITP. PMID: 26501552
    32. High expression of p15 antisense RNA is associated with acute myeloid leukemia. PMID: 27060304
    33. CDKN2B variants are associated with pediatric B-cell precursor acute lymphoblastic leukemia. PMID: 26868379
    34. Loss of CDKN2B may not only promote cardiovascular disease through the development of atherosclerosis but may also impair TGFbeta signaling and hypoxic neovessel maturation. PMID: 26596284
    35. CD93 regulates LSC self-renewal by silencing CDKN2B in MLL leukemia PMID: 26387756
    36. The treatment of Raji cell line with 10 muM curcumin caused hypomethylation of the p15 promoter after six days. This led to the up-regulation of p15 and down-regulation of DNA methyltransferase 1. PMID: 26333125
    37. Findings identify germline CDKN2B mutations as a novel cause of familial renal cell carcinoma. PMID: 25873077
    38. There was no association between germline coding CDKN2B variants and B cell acute lymphoblastic leukemia in children. PMID: 26104880
    39. Results show that ANRIL positively regulates the proliferation of cancer cells, via regulating p15 and other genes related to G2/M phase control. PMID: 26408699
    40. these data show that DC-SCRIPT acts as a novel regulator of CDKN2B and induces cell cycle arrest in ESR1-positive breast cancer cells. PMID: 25663546
    41. silencing of p15 gene expression by epigenetic modification such as promoter hypermethylation plays a role not only in the initiation of multiple myeloma but also in plasma cell malignant transformation, disease progression, and development PMID: 25382971
    42. promoter methylation of p15 gene could have prognostic value in diffuse large B-cell lymphoma patients. PMID: 24815848
    43. CDKN2B locus deletion is associated with melanoma brain metastases. PMID: 24968695
    44. genotypes of the polymorphic markers of KCNJ11, SLC30A8 and CDKN2B genes showed the presence of association with T2DM in Russian population, while for the FTO gene was not found statistically significant associations with type 2 diabetes PMID: 25916116
    45. CDKN2B polymorphism is associated with the development of coronary atherosclerosis. PMID: 24573017
    46. Single nucleotide polymorphisms in CDKN2B gene are associated with cancer. PMID: 25239644
    47. Exogenous p15 protein expression combined with STI571 appeared to have a synergistic effect on proliferation inhibition and apoptosis induction in K562 cells. PMID: 24938701
    48. Investigate the relationship between urinary nickel and methylation of p16 in workers exposed to nickel. The levels of urinary nickel were significantly associated with the methylation of p15. PMID: 24806561
    49. CDKN2B upregulation was found in myelodysplastic syndrome mesenchymal stem cells. PMID: 24943834
    50. Loss of p15INK4b was associated with older age. PMID: 24716938

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  • 亚细胞定位：
    Cytoplasm. Note=Also found in the nucleus.
  • 蛋白家族：
    CDKN2 cyclin-dependent kinase inhibitor family
  • 组织特异性：
    Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.
  • 数据库链接：

    HGNC: 1788

    OMIM: 600431

    KEGG: hsa:1030

    STRING: 9606.ENSP00000276925

    UniGene: Hs.72901