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  4. Recombinant Human Cystathionine gamma-lyase (CTH)

Recombinant Human Cystathionine gamma-lyase (CTH)

  • 货号：
    CSB-YP006160HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006160HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006160HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006160HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006160HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CTH
  • Uniprot No.：
  • 别名：
    CGL_HUMAN; CTH; cystathionase (cystathionine gamma-lyase); Cystathionine gamma lyase; Cystathionine gamma-lyase; Cysteine desulfhydrase; Gamma cystathionase; Gamma-cystathionase; Homoserine deaminase; Homoserine dehydratase; MGC9471
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-405
  • 氨基酸序列
    MQEKDASSQG FLPHFQHFAT QAIHVGQDPE QWTSRAVVPP ISLSTTFKQG APGQHSGFEY SRSGNPTRNC LEKAVAALDG AKYCLAFASG LAATVTITHL LKAGDQIICM DDVYGGTNRY FRQVASEFGL KISFVDCSKI KLLEAAITPE TKLVWIETPT NPTQKVIDIE GCAHIVHKHG DIILVVDNTF MSPYFQRPLA LGADISMYSA TKYMNGHSDV VMGLVSVNCE SLHNRLRFLQ NSLGAVPSPI DCYLCNRGLK TLHVRMEKHF KNGMAVAQFL ESNPWVEKVI YPGLPSHPQH ELVKRQCTGC TGMVTFYIKG TLQHAEIFLK NLKLFTLAES LGGFESLAEL PAIMTHASVL KNDRDVLGIS DTLIRLSVGL EDEEDLLEDL DQALKAAHPP SGSHS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
  • 基因功能参考文献：
    1. We also performed SNP analysis of H2S synthesizing enzymes and found a significant increase in cystathionine gamma-lyase (CTH) 1364G-T allele frequency in patients with cardiovascular disease compared to controls PMID: 29413960
    2. CSE is expressed in intraplaque microvessels, and H2S is a stimulator of micro-angiogenesis in vitro. Due to this pro-angiogenic effect, high levels of CSE in atherosclerotic plaques may be a potential risk for plaque vulnerability. PMID: 27708362
    3. Study demonstrated that lower abundance of cystathionine gamma-lyase expression and H2S production enhances type-2 immunoreaction and renders a higher incidence of allergic asthma at a young age. PMID: 28253731
    4. MicroRNA-186 promotes macrophage lipid accumulation and pro-inflammatory cytokine secretion by targeting cystathionine gamma-lyase in THP-1 macrophages PMID: 27205869
    5. not upregulated in uterine artery endothelium and smooth muscle in estrogen-dominant physiological states PMID: 28339573
    6. Inducible expression of CBS and CSE was found to be associated with a mineralizing phenotype in mesenchymal stem cells transitioning to mineralizing osteoblasts. PMID: 28121025
    7. ur findings suggest that these two families of amines inhibit cystathionine-gamma-lyase (CSE) to varying extents, which directly results in altered levels of intracellular HCY and consequent changes in Human vascular smooth muscle cells proliferation. PMID: 28034716
    8. Lipopolysaccharide increases the expression of CSE in human macrophages, increasing H2S synthesis, via ERK/NF-kappaB pathway. PMID: 26631093
    9. Nox4 is a positive transcriptional regulator of cystathionine-gamma-lyase in endothelial cells. PMID: 26620565
    10. The expression of CSE was positively correlated with the severity of gastric ulcer PMID: 26060478
    11. Our study demonstrates that CSE/H2S system is regulated by miR-216a, and regulates ABCA1-mediated cholesterol efflux and cholesterol levels through the PI3K/AKT pathway. PMID: 26772887
    12. Collectively, our findings indicate that radiation could promote HCC cell invasion through EMT mediated by endogenous H2S/CSE signaling via the p38MAPK pathway. PMID: 26727544
    13. 3-Mercaptopyruvate sulphurtransferase and not cystathionine gamma-lyase is the primary regulator of coronary artery hydrogen sulfide production and function. PMID: 26519030
    14. An increase of placental mRNA levels in the pre-eclampsia group was observed for methionine synthase and cystathionine gamma-lyase. PMID: 25801727
    15. GPBAR1 plays a role in secondary bile acid induced vasodilation via reglation of cystathionine gamma-lyase. The GPBAR1/CSE pathway might contribute to endothelial dysfunction and hyperdynamic circulation in liver cirrhosis. PMID: 25934094
    16. The miR-30 family are potentially important regulators of cystathionine gamma-lyase gene expression. PMID: 25203395
    17. The l-cysteine/cystathionine gamma lyase/hydrogen sulfide pathway is involved in melanoma progression. PMID: 25205294
    18. Substantial portion of Huntington's disease neurotoxicity appears to be attributable to cystathionine gamma-lyase deficiency. PMID: 25486189
    19. CTH is distributed in the human fallopian tube epithelium. PMID: 24914509
    20. Wnt pathway regulates CSE gene expression on transcriptional level and CSE/hydrogen sulfide plays important roles in colon cancer. PMID: 25193114
    21. These results demonstrate that H2S/CSE and its downstream pathway contribute to the proliferation of hepatoma cells, and inhibition of this pathway strongly suppress the excessive growth of hepatoma cells by stimulating mitochondrial apoptosis PMID: 24657251
    22. CTH observed relationship between the rs482843 polymorphism and the risk of preeclampsia. PMID: 25807836
    23. our data suggest that the NF-kappaB binding site on CSE promoter is critical for LPS-induced CSE expression in mammalian cells. PMID: 24866963
    24. Studies indicate taht a number of transcript factors regulate cystathionine gamma-lyase (CSE) transcription through direct or indirect binding with CSE promoter. PMID: 24896355
    25. The up-regulation of cystathionine gamma-lyase expression during hypoxia may be useful for increasing the production and concentration of H2S in mammalian cells and indirectly protecting cells from hypoxia. PMID: 23852134
    26. results of the present study provide molecular insights into the antioxidative activity of CSE and highlights the importance of the CSE/H2S system in maintaining cellular glutathione status PMID: 24707893
    27. demonstrates for the first time that both hyperglycemia and hyperketonemia mediate a reduction in CSE expression and activity, which can contribute to the impaired H2S signaling associated with diabetes PMID: 24610811
    28. Overexpression of the gene encoding CTH in cells leads to increased production of H2S, which plays a role in neuron protection against oxidative stress, and stimulates increase in gamma-glutamylcysteine synthetase and an increase in level of GSH. (review) PMID: 24491890
    29. Endogenous H2S is required for healthy placental vasculature, and a decrease in cystathionine gamma-lyase/H2S activity may contribute to the pathogenesis of preeclampsia. PMID: 23704251
    30. MicroRNA-21, which negatively regulates CSE expression, was increased in placentas with abnormal Doppler waveforms. PMID: 23410520
    31. Transgenic CTH plays a critical role in the preservation of cardiac function following transverse aortic constriction, i.e., in the setting of pressure overload-induced heart failure. PMID: 23393010
    32. CTH 1208 GT genotype was associated with increased chance of pregnancy and smaller number of previously failed IVF cycles and genotype frequency was lower in IVF patients with three or more previously failed IVF treatments compared with fertile controls PMID: 21507721
    33. Take together, these results suggest that miR-21 participates in CSE/H(2)S-mediated-SMC differentiation by targeting SP1. PMID: 22034194
    34. PI3K/AKT pathway increased the activity of cystathionine gamma-lyase gene promoter via Sp1. PMID: 22360859
    35. CTH is present in prostatic tissues and both normal and malignant prostate cell lines, including stromal compartments and the stromal cell line WPMY-1. It is downregulated by dihydrotestosterone. PMID: 22310774
    36. H(2)S generated by CSE and CBS locally exerts dual effects on the contractility of pregnant myometrium PMID: 21886822
    37. transcript factor Sp1 is a critical regulator of the hCSE expression during SMC differentiation, and CSE/H(2)S system is essential for maintenance of SMC phenotype. PMID: 21659522
    38. The investigations could aim at verifying whether the stimulation of CST, at the level of protein or gene expression, could change the proliferation of neoplastic cells. PMID: 20446008
    39. the CTH p.T67I substitution could have an ancient common origin, which probably occurred in the Neolithic Era and spread throughout Europe. PMID: 20584029
    40. The increased expression of cystathionine-gamma-lyase(CSE)/H2S pathway might be involved in the pathogenesis of viral myocarditis. PMID: 20849728
    41. Cystathionine beta-synthase 844Ins68 polymorphism is not associated with the levels of homocysteine and cysteine in an Indian population PMID: 20175737
    42. two nonsense mutations, namely exon 8 c.940-941delCT and exon 11 c.1220delC, and two missense mutations, namely exon 2 c.356C>T (T67I) and exon 7 c.874C>G (Q240E)were found in four probands with cystathioninuria PMID: 12574942
    43. Cystathionine gamma-lyase has a role in regulating cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/WAK-1 PMID: 15347670
    44. Mutations weaken the affinity for pyridoxal-5-phosphate and suggest that cystathionuric patients with these mutations should be responsive to pyridoxine therapy. PMID: 18476726
    45. The present study suggests that the SNPs rs482843 and rs1021737 of the CTH gene were not associated with essential hypertension in the Northern Chinese Han population. PMID: 18701025
    46. porphyrin moiety of the heme plays a critical role in proper CBS folding and assembly, but the metal ion is not essential for this function or for allosteric regulation by S-adenosyl-L-methionine PMID: 18849566
    47. structure of hCSE.PLP.PAG complex highlights the particular importance of Tyr(114) in hCSE and the mechanism of PAG-dependent inhibition of hCSE PMID: 19019829
    48. H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia PMID: 19261609
    49. These results demonstrate that cystathionase is a farnesoid X receptor-regulated gene and provide a new molecular explanation for the pathophysiology of portal hypertension. PMID: 19418582
    50. no evidence that severe loss of CTH activity due to the mutations is accompanied by adverse clinical effects PMID: 19428278

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  • 相关疾病：
    Cystathioninuria (CSTNU)
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Trans-sulfuration enzymes family
  • 数据库链接：

    HGNC: 2501

    OMIM: 219500

    KEGG: hsa:1491

    STRING: 9606.ENSP00000359976

    UniGene: Hs.19904