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  4. Recombinant Human Cytochrome P450 2S1 (CYP2S1)

Recombinant Human Cytochrome P450 2S1 (CYP2S1)

  • 中文名称：
    Recombinant Human Cytochrome P450 2S1(CYP2S1),Yeast
  • 货号：
    CSB-YP857028HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Cytochrome P450 2S1(CYP2S1),Yeast
  • 货号：
    CSB-EP857028HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Cytochrome P450 2S1(CYP2S1),Yeast
  • 货号：
    CSB-EP857028HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Cytochrome P450 2S1(CYP2S1),Yeast
  • 货号：
    CSB-BP857028HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Cytochrome P450 2S1(CYP2S1),Yeast
  • 货号：
    CSB-MP857028HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CYP2S1
  • Uniprot No.：
  • 别名：
    CP2S1_HUMAN; CYP2S1; CYPIIS1; Cytochrome P450 2S1; cytochrome P450; family 2; subfamily S; polypeptide 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-504
  • 氨基酸序列
    MEATGTWALL LALALLLLLT LALSGTRARG HLPPGPTPLP LLGNLLQLRP GALYSGLMRL SKKYGPVFTI YLGPWRPVVV LVGQEAVREA LGGQAEEFSG RGTVAMLEGT FDGHGVFFSN GERWRQLRKF TMLALRDLGM GKREGEELIQ AEARCLVETF QGTEGRPFDP SLLLAQATSN VVCSLLFGLR FSYEDKEFQA VVRAAGGTLL GVSSQGGQTY EMFSWFLRPL PGPHKQLLHH VSTLAAFTVR QVQQHQGNLD ASGPARDLVD AFLLKMAQEE QNPGTEFTNK NMLMTVIYLL FAGTMTVSTT VGYTLLLLMK YPHVQKWVRE ELNRELGAGQ APSLGDRTRL PYTDAVLHEA QRLLALVPMG IPRTLMRTTR FRGYTLPQGT EVFPLLGSIL HDPNIFKHPE EFNPDRFLDA DGRFRKHEAF LPFSLGKRVC LGEGLAKAEL FLFFTTILQA FSLESPCPPD TLSLKPTVSG LFNIPPAFQL QVRPTDLHST TQTR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids. In epidermis, may contribute to the oxidative metabolism of all-trans-retinoic acid. For this activity, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Additionally, displays peroxidase and isomerase activities toward various oxygenated eicosanoids such as prostaglandin H2 (PGH2) and hydroperoxyeicosatetraenoates (HPETEs). Independently of cytochrome P450 reductase, NADPH, and O2, catalyzes the breakdown of PGH2 to hydroxyheptadecatrienoic acid (HHT) and malondialdehyde (MDA), which is known to act as a mediator of DNA damage.
  • 基因功能参考文献：
    1. These results suggest that oxaliplatin exerts its inhibitory effects in human CRC cells via upregulation of CYP2S1 expression in a p53-dependent manner. PMID: 27609465
    2. CYP2S1 regulates colorectal cancer growth through associated with prostaglandin E2-mediated activation of beta-catenin signaling. PMID: 25557876
    3. The P450 2S1 may be involved in the reductive detoxication of several of the activated products of carcinogenic aromatic amines and heterocyclic aromatic amines. PMID: 23682735
    4. NADPH-P450 reductase PMID: 23648403
    5. found that CYP inhibition and siRNA-mediated downregulation of CYP2S1 increased macrophage phagocytosis PMID: 23224081
    6. The reduction in CYP2S1 expression doubled intracellular PGE(2) levels. PMID: 22863683
    7. Data suggest that CYP2S1 is induced in a keratinocyte cell line by exposure to all-trans retinoic acid and ultraviolet B radiation. PMID: 22039172
    8. No association has been found between the CYP2J2 (rs890293, -76G > T) or CYP2S1 (rs34971233, 13106C > T, P466L and rs338583, 13255A > G) gene polymorphisms and respiratory diseases. PMID: 22232929
    9. P450 2S1 can be reduced by NADPH-P450 reductase and suggest normal mixed-function oxidase roles of P450 2S1 to be revealed. PMID: 21430234
    10. identify a novel regulatory cassette that mediates changes in Cyp2s1 expression PMID: 17277313
    11. The presence of a CYP2A-related protein and testosterone metabolism in stellate cell cultures suggest that stellate cells express specific functional isoforms of CYP of which a major form is CYP2S1 PMID: 17280660
    12. identified the following three novel single nucleotide polymorphisms (SNPs): 4612G>A (Glu147Glu) in exon 3, 5478C>T (Leu230Leu) and 5479T>G (Leu230Arg, CYP2S1*5A) in exon 5 in Japanese individuals PMID: 17495422
    13. 12 genetic variations, which included the two novel nonsynonymous mutations CYP2S1 S61N (0.3%) and CYP2S1 L230R (0.8%), were identified in 50 Korean subjects PMID: 17529885
    14. Review compares the expression of CYP2S1 mRNA and protein in humans, mice and rats, and critically examines evidence pertaining to CYP2S1 regulation and its catalytic activity. PMID: 19368491
    15. CYP2S1 contributes to the metabolism of environmental carcinogens via a nicotinamide adenine dinucleotide phosphate (NADPH)- independent activity. PMID: 19713358

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Expressed at higher levels in extrahepatic tissues including trachea, lung, stomach, small intestine, colon, kidney, breast, placenta and spleen. Expressed in peripheral blood leukocytes. Constitutively expressed in skin (at protein level).
  • 数据库链接：

    HGNC: 15654

    OMIM: 611529

    KEGG: hsa:29785

    STRING: 9606.ENSP00000308032

    UniGene: Hs.98370