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  4. Recombinant Human Cytochrome b-245 light chain (CYBA)

Recombinant Human Cytochrome b-245 light chain (CYBA)

  • 货号：
    CSB-YP006323HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006323HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006323HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006323HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006323HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CYBACytochrome b-245 light chain; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-195
  • 氨基酸序列
    GQIEWAMWA NEQALASGLI LITGGIVATA GRFTQWYFGA YSIVAGVFVC LLEYPRGKRK KGSTMERWGQ KYMTAVVKLF GPFTRNYYVR AVLHLLLSVP AGFLLATILG TACLAIASGI YLLAAVRGEQ WTPIEPKPRE RPQIGGTIKQ PPSNPPPRPP AEARKKPSEE EAAVAAGGPP GGPQVNPIPV TDEVV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
  • 基因功能参考文献：
    1. Metabolic syndrome in Brazilian NAFLD patients most likely results from common allelic variants in a large number of genes, including CYBA and NOX4, that interact with each other, each of which alone determines a modest risk. PMID: 30087027
    2. The TT genotype of rs4673 in the CYBA gene was associated with Diabetic Peripheral Neuropathy in Type 1 Diabetes patients (OR 4.997, 95% CI 1.403-19.083, p = 0.016). PMID: 29924645
    3. T allele carriers of C242T gene polymorphism might be predisposed to overt diabetic nephropathy [meta-analysis] PMID: 27926811
    4. Three new mutations of CYBA gene in four of 22 Iranian patients with autosomal recessive-Chronic granulomatous disease were found. PMID: 28941186
    5. Data indicate an association between the GA genotype of single nucleotide polymorphism rs3794624 in cytochrome b-245, alpha polypeptide (CYBA) with decreased tuberculosis susceptibility in two Chinese populations. PMID: 27901128
    6. in patients with very severe chronic obstructive pulmonary disease the NADPH oxidase subunit p22phox is significantly reduced as compared to controls; p22phox is a key player in COPD and in hypoxic pulmonary vascular remodelling PMID: 28729471
    7. data demonstrated that rs4673 transition in p22phox gene may be involved in susceptibility to coronary artery disease and could be applied as a potential biomarker for this disease. PMID: 28474233
    8. Data suggest that an SNP in NADPH oxidase p22phox (C242T) is associated with nephropathy leading to macroalbuminuria in diabetic patients; this report is a meta-analysis of case-control genetic association studies. [META-ANALYSIS] PMID: 28457704
    9. Together with the increased p22phox expression in lungs of asthmatic patients, findings demonstrate a crucial role of p22phox-dependent NADPH oxidase for the development of mucus hypersecretion and airway hyperresponsiveness in house dust mite-induced model of asthma. PMID: 28510479
    10. Results showed that variations of the C242T polymorphism of the CYBA gene altered the risk of developing neonatal respiratory distress syndrome, retinopathy of prematurity, and bronchopulmonary dysplasia. PMID: 28375031
    11. Suggest that the C242T gene polymorphism is associated with arterial stiffness. Additionally, this relationship could be modified by smoking dose. PMID: 26467818
    12. In a family study of a patient with chronic granulomatous disease, the mutation in the CYBB gene was confirmed to be pathogenic, and the three variants in the CYBA gene were benign. PMID: 29132304
    13. We demonstrated that rapid deletion of p22phox is possible and that the activity of Nox1 and Nox4 but not Nox5 exclusively depends on p22phox. PMID: 27614387
    14. NOX5-p22phox complex drives monocytic differentiation into dendritic cells, and thus could be critical for immunity and inflammation. PMID: 28830888
    15. PI3K/AKT signaling only occurs when FLT3-ITD is expressed at the plasma membrane and is required for the production of NOX-generated ROS. ER retention of FLT3-ITD resulted in NOX4 deglycosylation and p22(phox) protein degradation. PMID: 27870947
    16. CYBA gene ()49A>G polymorphism modifies the risk of coronary artery disease PMID: 27314008
    17. The study demonstrated that the genetic variants of rs9932581 and rs1049255 in CYBA might not be associated with preeclampsia. PMID: 27607450
    18. p22phox C242T polymorphism has a possible role in changing the genetic susceptibility to late-onset AD in ApoE 4 carriers of northern Han Chinese origin. PMID: 26000926
    19. CYBA mutations lead to one of the autosomal recessive forms of chronic granulomatous disease (AR220CGD) clinically characterized by recurrent and severe infections in early chilA large number of genetic variations of CYBA have been reported, among them the C242T polymorphism, which has been extensively studied in association with coronary artery and heart diseases, but conflicting results continue to be reported. [Review] PMID: 27048830
    20. C242T single-nucleotide polymorphism causes p22(phox) structural changes that inhibit endothelial Nox2 activation and oxidative response to tumor necrosis factor-alpha or high-glucose stimulation. C242T single-nucleotide polymorphism may represent a natural protective mechanism against inflammatory cardiovascular diseases. PMID: 27162237
    21. CYBA C242T correlates with microalbuminuria onset in the French DT1 cohort. PMID: 26607824
    22. Overexpression of p22phox is associated with increased migration/metastasis rate in melanoma. PMID: 26760964
    23. To evaluate the association between CYBA gene polymorphisms and ESRD, we genotyped five CYBA polymorphisms. Our results suggested that rs1049255 polymorphism of CYBA modified the risk of ESRD. PMID: 26627442
    24. Substance P enhances tissue factor release from granulocyte-macrophage colony-stimulating factor-dependent macrophages via the p22phox/beta-arrestin 2/Rho A signaling pathway. PMID: 26852662
    25. Downregulating p22phox ameliorates inflammatory response in Angiotensin II-induced oxidative stress by regulating MAPK and NF-kappaB pathways in retinal pigment cells. PMID: 26415877
    26. TRX-1/PRX-1 levels are associated with NADPH oxidase-activity in vivo and in vitro in atherosclerosis. PMID: 26117319
    27. A functional variant in the promoter of the CYBA gene is associated with reduced renal function and with prevalence and incidence of diabetic nephropathy and end-stage renal disease in type 1 diabetic patients. PMID: 25862415
    28. C936T polymorphism of the VEGF gene and the C242T polymorphism of the p22phox gene are not correlated with diabetes mellitus type 2 and distal diabetic polyneuropathy. PMID: 26130419
    29. increased levels of gp91phox, p47phox and p22phox likely account for the interferon-gamma mediated enhancement of dimethyl sulfoxide-induced Nox2 activity. PMID: 26317224
    30. Data show that p22phox expression correlated with Epstein-Barr virus (EBV) and its encoded oncoprotein, latent membrane protein 1 (LMP1) expression. PMID: 26244812
    31. results suggest the evaluated NOX4 and CYBA SNPs are not direct genetic determinants of fibrosis in patients with chronic hepatitis C PMID: 25888935
    32. the C242T polymorphism might be a protective factor against developing acute coronary syndrome in the Asian population [meta-analysis] PMID: 25990054
    33. the A640G polymorphism may play a protective role in coronary heart disease. PMID: 24345348
    34. The analysis of covariance revealed that CYBA risk alleles and their haplotypes, were positively correlated with clinical parameters, for example, systolic blood pressure, diastolic blood pressure and mean arterial pressure, and biochemical parameters PMID: 25787042
    35. The A930G polymorphism of the p22phox gene may affect the susceptibility to ICH and certain haplotypes of the gene may be associated with a higher susceptibility to ICH. PMID: 25572489
    36. CDDP entry into the nucleus was severely impaired in p22phox-overexpressing cells (P < 0.001), and cytoplasmically accumulated CDDP was co-localized with overexpressed p22phox. PMID: 25686830
    37. our present analysis reinforces the involvement in ACT of the regulatory NADPH oxidase subunit RAC2 gene variant rs13058338 and, to a lesser extent of the CYBA gene variant rs4673. PMID: 25823784
    38. p22phox C242T polymorphism may have a role in ischemic cerebrovascular disease [meta-analysis] PMID: 25619262
    39. Associations between the CYBA 242C/T and the MPO -463G/A polymorphisms, oxidative stress and cardiovascular disease in chronic kidney disease patients. PMID: 17337886
    40. These observations clarify the effects of the studied polymorphisms on glomerular filtration rate and exemplify gene-gene interactions influencing renal function. PMID: 24890187
    41. Although our data suggest that expression of the gene encoding p22phox is not associated with chronic inflammation in patients with ulcerative colitis, other mechanisms can affect oxidative stress in these patients PMID: 25599773
    42. These results suggest that the recipients' p22(phox) C242T polymorphism may be a major risk factor for DGF in renal transplantation. PMID: 25173715
    43. Data show that NADPH oxidase NOX4 and p22(phox) localize to the nuclear membrane in MV4-11 leukemia cells expressing internal tandem duplication of the FMS-like tyrosine kinase (FLT3-ITD) receptor. PMID: 25697362
    44. The CYBA C-242T and CAT C-262T genetic polymorphisms and their epistatic interactions can be associated with ICC through mechanisms related with the role of ROS in cell proliferation and apoptosis. PMID: 25307973
    45. p22-phox 242T allele is associated with higher blood pressure levels among subjects with higher urinary sodium exretion in an urban Brazilian population PMID: 24339896
    46. A possible role of the first transmembrane domain (Trp18), and the region between the membrane and the dehydrogenase domain of NOX2 (Glu309, Ile325 and Gly389), in the binding with p22phox. PMID: 25252997
    47. A meta-analysis indicates that the NAD(P)H oxidase P22 phox gene 242 T allele might be associated with an increased type 2 diabetes mellitus risk. PMID: 24156725
    48. Homocysteine-induced expression of p22(phox) is regulated by the reactive oxygen species/p38MAPK pathway and PPARdelta activation is capable of attenuating this pathway by eliminating Hcy-induced reactive oxygen species production. PMID: 24486703
    49. In conclusion the -930A>G CYBA polymorphism is associated with CAD in the Polish population. The -930G allele carriers are particularly at risk of consequences of obesity and tobacco smoke exposure. PMID: 24477591
    50. C242T polymorphism of p22phox gene of NADPH oxidase is a novel genetic marker associated with reduced susceptibility to AMI. PMID: 24415302

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  • 相关疾病：
    Granulomatous disease, chronic, cytochrome-b-negative, autosomal recessive (ARCGD)
  • 亚细胞定位：
    Cell membrane.
  • 蛋白家族：
    P22phox family
  • 数据库链接：

    HGNC: 2577

    OMIM: 233690

    KEGG: hsa:1535

    STRING: 9606.ENSP00000261623

    UniGene: Hs.513803