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  4. Recombinant Human Cytoskeleton-associated protein 2 (CKAP2)

Recombinant Human Cytoskeleton-associated protein 2 (CKAP2)

  • 中文名称：
    Recombinant Human Cytoskeleton-associated protein 2(CKAP2),Yeast
  • 货号：
    CSB-YP840993HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Cytoskeleton-associated protein 2(CKAP2),Yeast
  • 货号：
    CSB-EP840993HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Cytoskeleton-associated protein 2(CKAP2),Yeast
  • 货号：
    CSB-EP840993HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Cytoskeleton-associated protein 2(CKAP2),Yeast
  • 货号：
    CSB-BP840993HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Cytoskeleton-associated protein 2(CKAP2),Yeast
  • 货号：
    CSB-MP840993HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CKAP2
  • Uniprot No.：
  • 别名：
    AI461656; AW228814; CKAP 2; Ckap2; CKAP2_HUMAN; CTCL tumor antigen se20 10; CTCL tumor antigen se20-10; Cytoskeleton associated protein 2; Cytoskeleton-associated protein 2; DKFZp686L1238; FLJ10749; LB1; se20 10; TMAP; Tumor and microtubule associated protein; Tumor associated microtubule associated protein; Tumor- and microtubule-associated protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-683
  • 氨基酸序列
    MSTPAVPQDL QLPPSQRAQS AFKEQRRQKL KEHLLRRKTL FAYKQENEML SSSRDQRVVT SEDQVQEGTK VLKLKTKMAD KENMKRPAES KNNTVVGKHC IPLKPSNELT NSTVVIDTHK PKDSNQTPHL LLTEDDPQSQ HMTLSQAFHL KNNSKKKQMT TEKQKQDANM PKKPVLGSYR GQIVQSKINS FRKPLQVKDE SSAATKKLSA TIPKATKPQP VNTSSVTVKS NRSSNMTATT KFVSTTSQNT QLVRPPIRSH HSNTRDTVKQ GISRTSANVT IRKGPHEKEL LQSKTALSSV KTSSSQGIIR NKTLSRSIAS EVIARPASLS NDKLMEKSEP VDQRRHTAGK AIVDSRSAQP KETSEERKAR LSEWKAGKGR VLKRPPNSVV TQHEPAGQNE KPVGSFWTTM AEEDEQRLFT EKVNNTFSEC LNLINEGCPK EDILVTLNDL IKNIPDAKKL VKYWICLALI EPITSPIENI IAIYEKAILA GAQPIEEMRH TIVDILTMKS QEKANLGENM EKSCASKEEV KEVSIEDTGV DVDPEKLEME SKLHRNLLFQ DCEKEQDNKT KDPTHDVKTP NTETRTSCLI KYNVSTTPYL QSVKKKVQFD GTNSAFKELK FLTPVRRSRR LQEKTSKLPD MLKDHYPCVS SLEQLTELGR ETDAFVCRPN AALCRVYYEA DTT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
  • 基因功能参考文献：
    1. CKAP2 acts as a functional oncogene in cervical carcinoma development and may exert its function by targeting FAK-ERK2 signaling pathway. PMID: 28522860
    2. Knockdown of CKAP2 expression effectively suppressed the proliferation and induced the apoptosis by inhibiting the phosphorylation of JAK2/STAT3 of osteosarcoma cells. PMID: 30119212
    3. High CKAP2 expression is associated with highgrade glioma. PMID: 30066946
    4. High CKAP2 expression is associated with Ovarian Cancer. PMID: 28933561
    5. Chromatin CKAP2 is an independent prognostic marker for relapse-free survival in early-stage breast cancer, and could potentially replace the mitotic activity index in clinical evaluation of proliferation activity. PMID: 24887265
    6. The transcriptional activity of RHOA, SEMA3B, and CKAP2 genes was assessed in blood samples of leukaemia patients and healthy donors. PMID: 24280143
    7. expression might be a potential biologic marker for identifying hepatocellular carcinoma patients at risk of early and extensive recurrence after operative resection PMID: 24238125
    8. CKAP2 is involved in the maintenance of microtubule nucleation sites PMID: 23737987
    9. GC box was responsible for the cyclic activity of human CKAP2 promoter through the phosphorylation of Sp1, possibly by Cyclin A/Cdk complex. PMID: 22465120
    10. Findings suggest that the motif surrounding Ser627 ((625) RRSRRL (630)) is a critical for kinase-substrate recognition and for regulation of the subcellular localization of TMAP during mitosis. PMID: 20458174
    11. TMAP is limited to pre-anaphase stages and suggest that the multiple phosphorylation may not act in concert but serve diverse functions. PMID: 19641375
    12. CKAP2 comprises nine exons ranging 70-1442 bp and is about 22 kb in size (regulatory regions included). The CKAP2 promoter contains CCAAT (-39...-33) rather than the canonical TATA box, and harbors nine binding sites for six transcription factors PMID: 12500535
    13. protein is expressed cell cycle dependently and it is involved in cell proliferation PMID: 16876122
    14. Data show that degradation of TMAP/CKAP2 during mitotic exit is mediated by the anaphase-promoting complex bound to Cdh1 and that the KEN box motif near the N terminus is necessary for its destruction. PMID: 17339342
    15. CKAP2 is a physiological substrate of anaphase-promoting complex/cyclosome during mitotic exit and that a tight regulation of the CKAP2 protein level is critical for the normal mitotic progression. PMID: 17376772
    16. Cdk1-cyclin B1-mediated phosphorylation of TMAP is important for and contributes to proper regulation of microtubule dynamics and establishment of functional bipolar spindles during mitosis PMID: 19369249

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
  • 蛋白家族：
    CKAP2 family
  • 组织特异性：
    Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
  • 数据库链接：

    HGNC: 1990

    OMIM: 611569

    KEGG: hsa:26586

    STRING: 9606.ENSP00000367276

    UniGene: Hs.444028