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  4. Recombinant Human DNA-3-methyladenine glycosylase (MPG), partial

Recombinant Human DNA-3-methyladenine glycosylase (MPG), partial

  • 中文名称：
    人MPG重组蛋白
  • 货号：
    CSB-YP014749HU
  • 规格：
    ￥1500
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    3 alkyladenine DNA glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; 3MG_HUMAN; AAG; ADPG; Alkyladenine DNA glycosylase; anpg; APNG; CRA36.1; DNA 3 methyladenine glycosylase; DNA-3-methyladenine glycosylase; MDG; Mid1; Mpg; N methylpurine DNA glycosirase; N methylpurine DNA glycosylase; N-methylpurine-DNA glycosylase; PIG11; PIG16; Proliferation inducing protein 11; Proliferation inducing protein 16
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    Yeast
  • 分子量：
    33.5kDa
  • 表达区域：
    13-298aa
  • 氨基酸序列
    QFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
  • 基因功能参考文献：
    1. high levels of APNG were associated with better overall survival in patients with glioblastoma PMID: 28662073
    2. Data indicate that DNA glycosylases MYH, UNG2, MPG, NTH1, NEIL1, 2 and 3 on nascent DNA. PMID: 28575236
    3. Data suggest that the change in tryptophan fluorescence of Y162W mutant of AAG (alkyladenine DNA glycosylase) is extremely rapid upon binding to either damaged or undamaged DNA, much faster than lesion-recognition and nucleotide flipping steps; thus, intercalation by tyrosine may be one of the earliest steps in search for/recognition of DNA damage. PMID: 28747435
    4. Rheumatoid arthritis is associated with a polymorphism in the MPG gene (rs2858056) and increased serum level of the MPG protein. PMID: 25757089
    5. Role of MPG protein in the DNA damage response through the base excision repair pathway PMID: 26025911
    6. results suggest that individuals carrying R120C and R141Q MPG variants may be at risk for genomic instability and associated diseases as a consequence. PMID: 25538240
    7. Elevated MPG activity is associated with lung cancer, possibly by creating an imbalance in the base excision repair pathway. PMID: 22266085
    8. In the case of alkyladenine DNA glycosylase, DNA intercalation contributes to the specific binding of a damaged nucleotide, but this enhanced specificity comes at the cost of reduced speed of nucleotide flipping. PMID: 25324304
    9. High MPG DNA repair assays for two different oxidative DNA lesions reveal associations with increased lung cancer risk. PMID: 25355292
    10. disease-stage-specific alterations in the expression of MPG may highlight a potential role for MPG in determining EAC onset and thus potentially be of clinical relevance for early disease detection and increased patient survival. PMID: 23137018
    11. AAG has a flexible amino terminus that tunes its affinity for nonspecific DNA, but we find that it is not required for intersegmental transfer. As AAG has only a single DNA binding site, this argues against the bridging model for intersegmental transfer PMID: 23839988
    12. AAG removes both methanol and 1,N(6)-ethenoadenine from DNA with single-turnover rate constants that are significantly greater than the corresponding uncatalyzed rates. PMID: 23688261
    13. Mitochondrial single-stranded binding protein (mtSSB) as a novel interacting partner of AAG. PMID: 23290262
    14. UHRF1 interacts with N-methylpurine DNA glycosylase (MPG) in cancer cells in vitro and displays a co-localization with MPG in the nucleoplasm. PMID: 23537643
    15. A functional footprinting approach was used to define the binding site of alkyladenine DNA glycosylase used for the repair of deaminated purines. PMID: 23074184
    16. N-methylpurine DNA glycosylase negatively regulates p53-mediated cell cycle arrest. PMID: 22801474
    17. AAG can make damaged DNA by catalyzing formation of an N-glycosyl bond between 1,N(6)-ethenoadenine (epsilonA) and abasic DNA. We attribute the reversibility of this reaction to the tight binding and slow subsequent hydrolysis of DNA containing an epsilonA lesion. PMID: 20873830
    18. Investigated the expression of MPG gene and protein in 128 glioma and 10 non-neoplastic brain tissues. Found MPG gene expression level in glioma tissues was significantly higher than that in non-neoplastic brain tissues (P < 0.001). PMID: 22496614
    19. The non-enzymatic binding of AAG to 3,N(4)-ethenocytosine specifically blocks ALKBH2-catalyzed repair of 3,N(4)-ethenocytosine but not that of methylated ALKBH2 substrates. PMID: 22079122
    20. Novel structures of AAG presented here help provide an understanding of this intriguing DNA repair protein, both in terms of understanding how AAG can recognize different types of DNA damage and in terms of how it may search the genome for DNA damage. PMID: 22148158
    21. Evaluation of APNG protein levels in several clinical datasets demonstrated that in patients, high nuclear APNG expression correlated with poorer overall survival compared with patients lacking APNG expression. PMID: 22156195
    22. The use of a concerted mechanism supports previous speculations that AAG uses a nonspecific strategy to excise both neutral 1,N(6)-ethenoadenine and cationic N(3)-methyladenine lesions. PMID: 21877721
    23. Structural basis for the inhibition of human alkyladenine DNA glycosylase (AAG) by 3,N4-ethenocytosine-containing DNA. PMID: 21349833
    24. Substitution of active site tyrosines with tryptophan alters the free energy for nucleotide flipping by human alkyladenine DNA glycosylase PMID: 21244040
    25. rs710079 and rs2858056 polymorphisms and the GCGC haplotype in the MPG gene are associated with the risk of rheumatoid arthritis progression. PMID: 21063071
    26. AAG uses hopping to effectively search both strands of a DNA duplex in a single binding encounter. PMID: 20201599
    27. The human alkyl-N-purine-DNA glycosylase (ANPG or MPG) excises both 1,N(6)-ethenoadenine and 1,N(2)-ethenoguanine adducts, exocyclic DNA adducts generated by lipid peroxidation, when present in DNA. PMID: 12016206
    28. The human alkyl-N-purine-DNA glycosylase (ANPG or MPG) excises with high efficiency hypoxanthine residues, deamination product of adenine, when present in DNA. ANPG is by far the most efficient hypoxanthine-DNA glycosylase of all the enzymes tested. PMID: 8016081
    29. The human alkyl-N-purine-DNA glycosylase (ANPG or MPG) binds tightly to ethenocytosine adduct when present in DNA. Unlike the ethenopurines, ANPG does not excise ethenocytosine but prevents its repair by forming an abortive protein-DNA complex. PMID: 14761949
    30. AAG and its mutants bind DNA containing one and two base-pair loops with significant affinity, thus shielding them from mismatch repair; the strength of such binding correlates with their ability to induce the mutator phenotype. PMID: 20347426
    31. evidence that the excised base rather than AP-site could be rate-limiting for DNA-glycosylase reactions PMID: 19616486
    32. Effects of hydrogen bonding within a damaged base pair on the activity of wild type and DNA-intercalating mutants of the human alkyladenine DNA glycosylase. PMID: 12077143
    33. MPG mRNA expression was slightly higher in astrocytic tumors than in adjacent tissue, suggesting a role in astrocytic tumors & the possibility that the altered MPG expression & intracellular localization could be associated with astrocytic tumorigenesis. PMID: 12820404
    34. Methylated DNA-binding domain 1 cooperates with this enzyme for transcriptional repression and DNA repair. PMID: 14555760
    35. alkyladenine DNA-glycosylase activates neutral lesions by protonation of the nucleobase leaving group PMID: 14567703
    36. analysis of substrate specificity of human 3-methyladenine-DNA glycosylase PMID: 14688248
    37. plays a role in maintaining integrity of the genome by recruiting DNA repair proteins to actively transcribing DNA [3-methyladenine DNA glycosylase] PMID: 14761960
    38. AAG is a mammalian enzyme that can act on all three purine deamination bases, hypoxanthine, xanthine, and oxanine PMID: 15247209
    39. C147G and C342G missense mutations and a 5'-UTR 1-27 insT were found in familial colorectal cancer DNA suggesting a limited role for this gene in the devlopment of CRC. PMID: 17029639
    40. this newly purified full-length hMPG is appreciably stable at high temperature, such as 50 degrees C. PMID: 18191412
    41. The mutability of the AAG substrate binding pocket, and the essentiality of individual binding pocket amino acids for survival of methylation damage, was assessed. PMID: 18706524
    42. Although the amino terminus of the protein is dispensable for glycosylase activity at a single site, we find that deletion of the 80 amino-terminal amino acids significantly decreases the processivity of AAG. PMID: 18839966
    43. Results suggest the possible significance of repair of the frequent lesions in single-stranded DNA transiently generated during replication and transcription. PMID: 19219989
    44. RNS-induced posttranslational modification of AAG is one mechanism of base excision repair dysregulation PMID: 19864471

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  • 亚细胞定位：
    Cytoplasm. Mitochondrion matrix, mitochondrion nucleoid. Nucleus.
  • 蛋白家族：
    DNA glycosylase MPG family
  • 数据库链接：

    HGNC: 7211

    OMIM: 156565

    KEGG: hsa:4350

    STRING: 9606.ENSP00000219431

    UniGene: Hs.459596