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  4. Recombinant Human DNA-binding protein RFX5 (RFX5)

Recombinant Human DNA-binding protein RFX5 (RFX5)

  • 中文名称：
    人RFX5重组蛋白
  • 货号：
    CSB-YP019614HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RFX5重组蛋白
  • 货号：
    CSB-EP019614HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RFX5重组蛋白
  • 货号：
    CSB-EP019614HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RFX5重组蛋白
  • 货号：
    CSB-BP019614HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RFX5重组蛋白
  • 货号：
    CSB-MP019614HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DNA binding protein RFX 5; DNA binding protein RFX5; DNA-binding protein RFX5; Influences HLA class II expression; Regulatory factor X 5; Regulatory factor X subunit 5; Regulatory factor X; 5 (influences HLA class II expression); RFX 5; RFX5; Rfx5 protein ; RFX5_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-616
  • 氨基酸序列
    AEDEPDAKS PKTGGRAPPG GAEAGEPTTL LQRLRGTISK AVQNKVEGIL QDVQKFSDND KLYLYLQLPS GPTTGDKSSE PSTLSNEEYM YAYRWIRNHL EEHTDTCLPK QSVYDAYRKY CESLACCRPL STANFGKIIR EIFPDIKARR LGGRGQSKYC YSGIRRKTLV SMPPLPGLDL KGSESPEMGP EVTPAPRDEL VEAACALTCD WAERILKRSF SSIVEVARFL LQQHLISARS AHAHVLKAMG LAEEDEHAPR ERSSKPKNGL ENPEGGAHKK PERLAQPPKD LEARTGAGPL ARGERKKSVV ESSAPGANNL QVNALVARLP LLLPRAPRSL IPPIPVSPPI LAPRLSSGAL KVATLPLSSR AGAPPAAVPI INMILPTVPA LPGPGPGPGR APPGGLTQPR GTENREVGIG GDQGPHDKGV KRTAEVPVSE ASGQAPPAKA AKQDIEDTAS DAKRKRGRPR KKSGGSGERN STPLKSAAAM ESAQSSRLPW ETWGSGGEGN SAGGAERPGP MGEAEKGAVL AQGQGDGTVS KGGRGPGSQH TKEAEDKIPL VPSKVSVIKG SRSQKEAFPL AKGEVDTAPQ GNKDLKEHVL QSSLSQEHKD PKATPP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
  • 基因功能参考文献：
    1. RFX5 acts as a direct positive transcriptional regulator of TPP1 in hepatocellular carcinoma. PMID: 27840983
    2. Data show arginine methyltransferase PRMT6 methylates the AT-hook motif of regulatory factor RFX5 and downregulates HLA-DQ expression. PMID: 23911394
    3. these data have identified as novel pathway whereby SIRT1 maintains COL1A2 synthesis in SMCs by modulating RFX5 activity. PMID: 23079621
    4. RFXAP(C) consists of two alpha-helices that form a V-shaped structure that packs within the RFX5(N)(2) staple. PMID: 20732328
    5. interaction at COL1A2 start site and transcription repression PMID: 11986307
    6. The mutation for the fifth Bbare lymphocyte syndrome complementation group was found in RFX5 and was mapped to one of the arginines in a DNA-binding surface of this protein. Its wild-type counterpart restored function. PMID: 12368908
    7. RFX5 binds to the collagen transcription start site and represses collagen gene expression PMID: 12968017
    8. The identification of nuclear import and export sites on RFX molecules provides potential targets to modulate MHC class II expression. PMID: 15210800
    9. RFXAP and RFXB have roles in relieving autoinhibition of RFX5 PMID: 18723135
    10. TSA treatment enhances the association of CIITA with the transcription factor RFX5, which ameliorates the down-regulation of CIITA recruitment to target promoters by HDAC2. PMID: 19041327

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  • 相关疾病：
    Bare lymphocyte syndrome 2 (BLS2)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RFX family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 9986

    OMIM: 209920

    KEGG: hsa:5993

    STRING: 9606.ENSP00000290524

    UniGene: Hs.632472