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  4. Recombinant Human DNA polymerase iota (POLI)

Recombinant Human DNA polymerase iota (POLI)

  • 货号：
    CSB-YP892157HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP892157HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP892157HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP892157HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP892157HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DNA polymerase iota; Eta 2; Eta2; POLI; POLI_HUMAN; Polymerase (DNA directed) iota; RAD 30B; RAD30 homolog B; RAD30, S. cerevisiae, homolog of; RAD30B; RAD3OB
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-740
  • 氨基酸序列
    MEKLGVEPEE EGGGDDDEED AEAWAMELAD VGAAASSQGV HDQVLPTPNA SSRVIVHVDL DCFYAQVEMI SNPELKDKPL GVQQKYLVVT CNYEARKLGV KKLMNVRDAK EKCPQLVLVN GEDLTRYREM SYKVTELLEE FSPVVERLGF DENFVDLTEM VEKRLQQLQS DELSAVTVSG HVYNNQSINL LDVLHIRLLV GSQIAAEMRE AMYNQLGLTG CAGVASNKLL AKLVSGVFKP NQQTVLLPES CQHLIHSLNH IKEIPGIGYK TAKCLEALGI NSVRDLQTFS PKILEKELGI SVAQRIQKLS FGEDNSPVIL SGPPQSFSEE DSFKKCSSEV EAKNKIEELL ASLLNRVCQD GRKPHTVRLI IRRYSSEKHY GRESRQCPIP SHVIQKLGTG NYDVMTPMVD ILMKLFRNMV NVKMPFHLTL LSVCFCNLKA LNTAKKGLID YYLMPSLSTT SRSGKHSFKM KDTHMEDFPK DKETNRDFLP SGRIESTRTR ESPLDTTNFS KEKDINEFPL CSLPEGVDQE VFKQLPVDIQ EEILSGKSRE KFQGKGSVSC PLHASRGVLS FFSKKQMQDI PINPRDHLSS SKQVSSVSPC EPGTSGFNSS SSSYMSSQKD YSYYLDNRLK DERISQGPKE PQGFHFTNSN PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
  • 基因功能参考文献：
    1. Findings indicate that DNA polymerase iota (poli) plays a positive role in lung cancer progression via promoting the migration and invasion of lung cancer cells. PMID: 30060029
    2. Findings indicate the function of tumor suppressor protein p53 (p53) and DNA polymerase iota (POLiota) in the DNA damage response to endogenous or exogenous replication stress. PMID: 27407148
    3. DNA polymerase iota increased the expression of MMP-2/9 to promote invasion and metastasis of esophageal squamous cell carcinoma. PMID: 27057634
    4. The predominant isoform of DNA poliota in human cells is the shorter 715 amino acid protein and that if, or when, expressed, the longer 740 amino acid isoform has identical properties to the considerably more abundant shorter isoform. PMID: 28865289
    5. Kinetic and Structural Impact of Metal Ions and Genetic Variations on Human DNA Polymerase iota. PMID: 27555320
    6. Mass spectrometry analysis of monoubiquitinated poliota revealed that it is ubiquitinated at over 27 unique sites. Many of these sites are localized in different functional domains of the protein. PMID: 26370087
    7. Germline genetic variations in human POLI gene may either hinder or promote the translesion synthesis (TLS) capability of pol iota with various DNA lesions in vitro, emphasizing the potential translational importance of these pol iota genetic variations, e.g., individual differences in TLS, mutation, and cancer susceptibility to genotoxic carcinogens. PMID: 25162224
    8. a single residue in pol iota is able to discriminate between NTPs and dNTPs during DNA synthesis. PMID: 24532793
    9. Dysregulation of pol iota by JNK/c-Jun is involved in carcinogenesis and offer a novel understanding of the role of pol iota or c-Jun in mutagenesis. PMID: 23922701
    10. Human Pol iota and yeast Pol zeta complex could function efficiently in the insertion and extension steps, respectively, of ranslesion synthesis and human Pol kappa and Pol eta could also extend past these lesions, albeit much less efficiently. PMID: 23965998
    11. POLI and MC4R nearby single nucleotide polymorphisms in human chromosome 18 are associated with a moderate risk of type 2 diabetes. PMID: 23727064
    12. Results show that the physical and functional interaction between pols eta and iota occurs between ubiquitinated forms of either polymerase via their respective ubiquitin-binding domains. PMID: 23248005
    13. this review briefly discusses the main structural features and possible functional mechanisms of the active center of Pol iota. [review] PMID: 22817454
    14. in mammalian cells, both polymerases kappa and iota are necessary for the error-free bypass of N(2)-CEdG and N(2)-CMdG. PMID: 21454642
    15. structural mechanism of high-fidelity 8-oxo-G replication by a human DNA polymerase PMID: 21300901
    16. Structural basis for proficient incorporation of dTTP opposite O6-methylguanine by human DNA polymerase iota. PMID: 20961860
    17. In the presence of Mn2+, the Pol iota activity in carcinoma was 2.5-fold higher than the control cells. PMID: 20673215
    18. The solution structures of the C-terminal UBM of human pol iota and its complex with ubiquitin are reported. PMID: 20159559
    19. Variants in POLI is associated with predisposition for TMPRSS2-ERG fusion in prostate cancer. PMID: 19861517
    20. DNA polymerase iota's versatility implies that this enzyme can adjust to local structural conditions in order to bypass bulky lesions by strategies that utilize Watson-Crick, Hoogsteen and perhaps other base pairing possibilities. PMID: 19767609
    21. By performing gene inactivation in a Burkitt's lymphoma cell line inducible for hypermutation, we show here that somatic hypermutation is dependent on DNA polymerase iota PMID: 12410315
    22. DNA polymerase iota associates with the replication machinery & accumulates at stalled replication forks following DNA-damaging treatment. Its the C-terminal 224 AAs are sufficient for both the interaction with poleta & accumulation in replication foci. PMID: 12426396
    23. Translesion replication of benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyadenosine and deoxyguanosine by DNA polymerase iota. PMID: 12466554
    24. pol iota can complement the in vitro single-nucleotide BER deficiency of a DNA polymerase beta null cell extract PMID: 12777390
    25. propose that the incipient base pair is accommodated differently in the active site of Poliota dependent upon the template base and that when T is the templating base, Poliota accommodates the wobble base pair better than the Watson-Crick base pair PMID: 14701763
    26. crystal structure bound to a template primer and an incoming nucleotide; structure reveals a polymerase that is 'specialized' for Hoogsteen base-pairing, whereby the templating base is driven to the syn conformation PMID: 15254543
    27. Data strongly suggest that pol iota may be involved in the generation of both increased spontaneous and translesion mutations during DNA replication in breast cancer cells, thereby contributing to the accumulation of genetic damage. PMID: 15313897
    28. Data show that an interaction between human DNA polymerase iota (poliota) and the proliferating cell nuclear antigen (PCNA) stimulates the processivity of poliota in a template-dependent manner in vitro. PMID: 15342632
    29. Poliota interacts with PCNA via only one of its conserved PCNA binding motifs, regardless of whether PCNA is bound to DNA or not. PMID: 15657443
    30. DNA polymerase iota promotes replication through a ring-closed minor-groove adduct that adopts a syn conformation in DNA PMID: 16166652
    31. The sequential action of Poliota and Polkappa promotes efficient and error-free synthesis through the HNE-dG adducts. PMID: 16354708
    32. identification of two previously unknown ubiquitin-binding domains in the Y-family translesion synthesis polymerases that enable them to interact with monoubiquitinated targets and undergo monoubiquitination in vivo PMID: 16357261
    33. results show that Pol iota has no significant role in UV lesion bypass and mutagenesis in vivo and provides some initial data suggesting that this polymerase may be involved in replication of extrachromosomal DNA PMID: 16472831
    34. polymerization by pol iota is severely inhibited by a bulky group at G N2 despite an advantageous mode of Hoogsteen base pairing PMID: 16527824
    35. DNA polymerases iota and eta interact noncovalently with free polyUb chains, as well as mono-ubiquitinated proliferating cell nuclear antigen (Ub-PCNA). PMID: 16763556
    36. For proficient T incorporation opposite template A, only the N7 hydrogen bonding is required, but for proficient C incorporation opposite template G, hydrogen bonding at both the N7 and O(6) is an imperative. PMID: 16914729
    37. These results suggest that HIF-1-mediated pol iota gene expression may be involved in the generation of translesion mutations during DNA replication after hypoxia followed by reoxygenation. PMID: 17056006
    38. the cation utilized by pol iota in vivo may actually be Mn(2+) rather than Mg(2+), as tacitly assumed PMID: 17609217
    39. Data suggest that DNA polymerases eta and iota transiently probe DNA/chromatin; when DNA is exposed at replication forks, the polymerase residence times increase, and this is further facilitated by the ubiquitination of PCNA. PMID: 18799611
    40. These data reveal a novel role of human DNA pol iota in protecting cells from oxidative damage. PMID: 18923427
    41. Lesion bypass of N2-ethylguanine by human DNA polymerase iota. PMID: 18984581
    42. enzyme plays important functions in protecting humans from the deleterious consequences of exposure to both oxidative- and ultraviolet light-induced DNA damage PMID: 19162565
    43. DNA synthesis across an absaic lesion by human POLI is reported. PMID: 19368886
    44. Structural and domain-swapping experiments indicate that the finger domain is responsible for DNA polymerase iota's high error rates on pyrimidines and determines the incorporation specificity. PMID: 19440206
    45. Replication across template T/U by human DNA POLI is reported. PMID: 19604477

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-Y family
  • 组织特异性：
    Ubiquitous. Highly expressed in testis.
  • 数据库链接：

    HGNC: 9182

    OMIM: 605252

    KEGG: hsa:11201

    STRING: 9606.ENSP00000462664

    UniGene: Hs.438533