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  4. Recombinant Human DNA repair protein RAD51 homolog 4 (RAD51D)

Recombinant Human DNA repair protein RAD51 homolog 4 (RAD51D)

  • 货号：
    CSB-EP019268HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    RAD51D
  • Uniprot No.：
  • 别名：
    BROVCA4; DNA repair protein RAD51 homolog 4; HsTRAD; OTTHUMP00000163851; OTTHUMP00000163852; OTTHUMP00000163853; R51H3; RA51D_HUMAN; RAD51 homolog D (S. cerevisiae); RAD51 homolog D; RAD51 like 3 (S. cerevisiae); RAD51 paralog D; RAD51, S. cerevisiae, homolog of, D; RAD51-like protein 3; Rad51l3; Recombination repair protein; S. cerevisiae RAD51-like 3; TRAD
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    62.0kDa
  • 表达区域：
    1-328aa
  • 氨基酸序列
    MGVLRVGLCPGLTEEMIQLLRSHRIKTVVDLVSADLEEVAQKCGLSYKALVALRRVLLAQFSAFPVNGADLYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCMAANVAHGLQQNVLYVDSNGGLTASRLLQLLQAKTQDEEEQAEALRRIQVVHAFDIFQMLDVLQELRGTVAQQVTGSSGTVKVVVVDSVTAVVSPLLGGQQREGLALMMQLARELKTLARDLGMAVVVTNHITRDRDSGRLKPALGRSWSFVPSTRILLDTIEGAGASGGRRMACLAKSSRQPTGFQEMVDIGTWGTSEQSATLQGDQT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
  • 基因功能参考文献：
    1. Contribution of RAD51D germline mutations in breast and ovarian cancer in Greece. PMID: 30111881
    2. Results highlight the importance of a functional RAD51D-XRCC2 interaction to promote HR and prevent the development of HGSC. PMID: 28646019
    3. variants in RAD51D were associated with moderate risks of breast cancer. PMID: 28418444
    4. of Rad51d mediated by E3 Ligase Rnf138 has a role in the homologous recombination repair pathway PMID: 27195665
    5. endogenous regulation of RAD51D by miR-103/107 was observed in several tumor subtypes; both miR-103 and miR-107 directly target and regulate RAD51 and RAD51D PMID: 24088786
    6. We aimed to determine the prevalence of germline RAD51D mutations in Spanish breast and ovarian cancer families negative for BRCA1/BRCA2 PMID: 24130102
    7. Our data provide additional evidence that RAD51D mutations are enriched among ovarian cancer patients, but are extremely rare among familial breast cancer patients. PMID: 23372765
    8. loss-of-function mutations in RAD51D predispose to ovarian carcinoma but not to breast carcinoma. PMID: 22986143
    9. RAD51D is primarily a moderate penetrance susceptibility gene for ovarian cancer, with clinical significance for the carriers. PMID: 22652533
    10. The RAD51D should be included in genetic screening of ovarian cancer families that do not have BRCA1/BRCA2 mutations. PMID: 22415235
    11. These data indicate that RAD51D mutation testing may have clinical utility in individuals with ovarian cancer and their families. PMID: 21822267
    12. The N-terminal domain of Rad51D is required for the ssDNA-specific binding function of human Rad51D. PMID: 21111057
    13. Polymorphisms in RAD51D gene is associated with breast cancer. PMID: 20054644
    14. Homologous pairing and ring and filament structure formation activities of the human Xrcc2*Rad51D complex PMID: 11834724
    15. RAD51L3 cooperates with Bloom Syndrome Protein during the late stages of homologous recombination processes that serve to restore productive DNA replication at sites of damaged or stalled replication forks PMID: 12975363
    16. Telomere maintenance requires RAD51D. PMID: 15109494
    17. E233G single nucleotide polymorphism is a low-penetrance susceptibility gene in the specific subgroup of high-risk familial breast cancer cases that are not related to BRCA1/2. PMID: 15170666
    18. Interactions between RAD51D and its XRCC2 and RAD51C partners require a functional RAD51D Walker B ATPase motif, but not motif A. PMID: 16717288
    19. The RAD51D E233G variant is not associated with breast cancer. PMID: 18058226

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  • 相关疾病：
    Breast-ovarian cancer, familial, 4 (BROVCA4)
  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, telomere.
  • 蛋白家族：
    RecA family, RAD51 subfamily
  • 组织特异性：
    Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes.
  • 数据库链接：

    HGNC: 9823

    OMIM: 602954

    KEGG: hsa:5892

    STRING: 9606.ENSP00000466834

    UniGene: Hs.631757