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  4. Recombinant Human DNA repair protein complementing XP-G cells (ERCC5), partial

Recombinant Human DNA repair protein complementing XP-G cells (ERCC5), partial

  • 货号：
    CSB-EP007773HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    COFS 3; COFS3; DNA excision repair protein ERCC 5; DNA excision repair protein ERCC-5; DNA excision repair protein ERCC5; DNA repair protein complementing XP G cells; DNA repair protein complementing XP-G cells; DNA repair protein complementing XPG cells; ERCC 5; ERCC5; ERCC5_HUMAN; ERCM 2; ERCM2; Excision repair cross complementation group 5; Excision Repair Cross Complementing Rodent Repair Deficiency; Excision repair cross complementing rodent repair deficiency complementation group 5; Excision repair protein; OTTHUMP00000064902; UVDR; Xeroderma Pigmentosum Complementation Group G; Xeroderma pigmentosum complementation group G protein; Xeroderma pigmentosum group G complementing protein; Xeroderma pigmentosum group G-complementing protein; XPG; XPG complementing protein; XPGC
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    30.8 kDa
  • 表达区域：
    947-1186aa
  • 氨基酸序列
    SFLWGKPDLDKIREFCQRYFGWNRTKTDESLFPVLKQLDAQQTQLRIDSFFRLAQQEKEDAKRIKSQRLNRAVTCMLRKEKEAAASEIEAVSVAMEKEFELLDKAKGKTQKRGITNTLEESSSLKRKRLSDSKGKNTCGGFLGETCLSESSDGSSSEDAESSSLMNVQRRTAAKEPKTSASDSQNSVKEAPVKNGGATTSSSSDSDDDGGKEKMVLVTARSVFGKKRRKLRRARGRKRKT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II. Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity. Required for DNA replication fork maintenance and preservation of genomic stability. Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site. During HRR, binds to the replication fork with high specificity and stabilizes it. Also, acts upstream of HRR, to promote the release of BRCA1 from DNA.
  • 基因功能参考文献：
    1. This meta-analysis indicates that the XPG rs751402 polymorphism may be a risk factor for gastric cancer in the Chinese population. PMID: 29148016
    2. There is a multifarious interaction between the DNA repair gene ERCC5 SNPs (rs2094258 and rs873601) and the metabolic gene GSTP1 rs1695, which may form the basis for various inter-individual susceptibilities to atrophic gastritis in Chinese population. PMID: 29434449
    3. this meta-analysis shows that XPG gene polymorphisms are associated with lung cancer and gastric cancer PMID: 28416771
    4. Nine case-control studies involving 3540 cases and 3953 controls were included in the meta-analysis, which revealed that the XPG rs751402 polymorphism is positively associated with GC risk and could be viewed as a risk factor of GC in three genetic models. The XPG gene rs751402 polymorphism is associated with an increased risk of GC in Chinese Han populations. This fi nding should be veri fi ed by larger studies. PMID: 28832189
    5. In NBEC, T allele at SNP rs2296147 upregulates ERCC5. PMID: 27235448
    6. XPG gene polymorphism rs751402 was associated with increased susceptibility to gastric cancer in Chinese populations (Meta-Analysis) PMID: 29049208
    7. Overexpression of human XPG and FEN1 increases genome instability in U2OS cells PMID: 28704715
    8. This study showed that XPG rs2296147 CT/TT variants conferred significant survival disadvantage in CRC patients in term of PFS. PMID: 26887052
    9. These results indicated that none of the selected XPG polymorphism could significantly alter gastric cancer susceptibility alone. PMID: 27929383
    10. meta-analysis suggested that the rs873601 polymorphism was significantly associated with overall cancer risk. The moderate effects of rs751402 and rs2296147 polymorphism on cancer susceptibility might be highly dependent on cancer type and ethnicity, respectively PMID: 28796034
    11. XPG mRNA expression was not predictive of trabectedin efficacy as single agent in hormone-positive, HER-2-negative advanced breast cancer. PMID: 27266804
    12. the XPG gene rs2094258 C>T polymorphism may contribute to neuroblastoma susceptibility. PMID: 27019310
    13. No strong evidence was found to support the use of XPG polymorphisms as tumor response and prognostic factors of patients with NSCLC receiving a platinum-based treatment regimen--(REVIEW) PMID: 28314991
    14. Xpg thus helps to adequately induce DNA damage responses after IR, thereby keeping the expansion of damaged cells under control. This represents a new function of Xpg in the response to IR, in addition to its well-characterized role in nucleotide excision repair. PMID: 27137888
    15. Meta-analysis indicated that the ERCC1 rs3212986 polymorphism and 2 polymorphisms in ERCC2 gene (rs13181 and rs1799793) contributed to the susceptibility of glioma. However, no association was observed between glioma risk and ERCC1 rs11615, ERCC2 rs238406, and ERCC5 rs17655 polymorphisms. PMID: 28514298
    16. The rs751402 C/T SNP T allele and the T/T genotype were associated with an increased risk of GCA in younger individuals (>61 years) (odds ratio [OR] = 1.33 and 1.77, 95% confidence interval [CI] = 1.00-1.76 and 1.12-3.30, respectively). The rs873601 G/A SNP was not associated with susceptibility to GCA. PMID: 27228234
    17. The ERCC5 rs751402 gene polymorphism may influence the susceptibility to gastric cancer in the Chinese population. PMID: 27706622
    18. we found that XPG rs2094258, rs751402, and rs17655 do not influence the development of breast cancer in a Chinese population PMID: 27323134
    19. The results from this meta-analysis indicate that the XPG gene Asp1104His polymorphism is associated with lung cancer risk, especially in Asians. PMID: 27323149
    20. ). In conclusion, we suggest that the rs2094258 and rs751402 polymorphisms of ERCC5 are not connected to the development of this disease under codominant, dominant, and recessive models. PMID: 27323158
    21. The ERCC5 promoter polymorphisms at -763 and +25 may be important functional variants and predictors of clinical outcome of advanced colorectal cancer patients who received oxaliplatin chemotherapy. PMID: 27175691
    22. our study suggests that the rs17655 polymorphism in XPG is associated with an increased risk of gastric cancer. The results of our findings should be further validated by further large sample size studies PMID: 27323165
    23. we suggest that the ERCC5 rs751402 polymorphism is associated with development of gastric cancer PMID: 27323183
    24. ERCC5 single nucleotide polymorphism rs751402 is associated with breast cancer characteristics and risk in the Han population of northwest China. PMID: 25644244
    25. The results indicate that XPG rs873601G>A polymorphism may be associated with the risk of stomach cancer. PMID: 26820236
    26. GG genotype of rs17655 was correlated with an increased risk of gastric cancer compared to the CC genotype. rs1047768 and rs751402 were not significantly correlated with gastric cancer risk. PMID: 27051028
    27. Results show that XPG partners with BRCA1 and BRCA2 to maintain genomic stability through homologous recombination, and its loss causes DNA breaks, chromosome aberrations, and replication fork stalling. PMID: 26833090
    28. ERCC5 rs17655 polymorphism might contribute to genetic susceptibility to colorectal cancer. PMID: 26225711
    29. XRCC1 Arg399Gln and XPG His46His might significantly affect the clinical outcomes of platinum-based chemotherapy. PMID: 24737519
    30. our study indicated that ERCC5 rs2094258 polymorphism may contribute to the risk of breast cancer. PMID: 26045839
    31. XPG Asp1104His polymorphism is a risk factor for head and neck cancer susceptibility. PMID: 25987016
    32. the rs2296147 and rs2094258 polymorphisms of XPG, could be used as surrogate markers, leading to individualization of non-small cell lung cancer treatment strategies. PMID: 25729984
    33. CDT2 mediated XPG elimination from DNA damage sites clears the chromatin space needed for repair. PMID: 25483071
    34. Individuals that harbor uORF1 of ERCC5 have a marked resistance to platinum-based agents PMID: 26338418
    35. conclusion, our findings suggest that XPG Asp1104His polymorphism may increase the susceptibility of CRC, especially in Asian populations. PMID: 25332048
    36. Meta-analysis suggests that XPF Arg415Gln polymorphism may be associated with decreased lung cancer risk and XPG Asp1104His may be a low-penetrant risk factor in some cancers development. PMID: 24802942
    37. Our data broaden the reported clinical spectrum of ERCC5 mutations and provide further evidence of genotype-phenotype correlation with truncating mutations being associated with severe phenotypes. PMID: 24700531
    38. A novel mutation in ERCC5, causative of a pellagra-like condition linked to xeroderma pigmentosum/Cockayne syndrome complex. PMID: 24354460
    39. This meta-analysis suggested that the XPG Asp1104His polymorphism was a risk factor for melanoma susceptibility. PMID: 25231183
    40. Results found that polymorphisms in XPG rs2296147 and CSB rs2228526 were significantly associated with prostate cancer susceptibility in the Chinese population analyzed. PMID: 24615090
    41. our study indicated that XRCC1 Arg399Gln and ERCC5 His46His might significantly influence the response to chemotherapy PMID: 24782167
    42. The XPC rs2228000 TT genotype is associated with shorter overall survival in gastric cancer. PMID: 24990617
    43. Three SNPs of XPG, rs2296147T>C, rs2094258C>T and rs873601G>A, were genotyped. PMID: 23464443
    44. study provided statistical evidence that XPG rs2296147T>C and rs873601G>A polymorphisms may be used as surrogate markers toward individualizing non-small cell lung cancer treatment strategies PMID: 24615519
    45. ERCC5 rs17655 polymorphism may not contribute to genetic susceptibility for lung cancer PMID: 24596032
    46. The XPG gene polymorphism was also related to bladder cancer yet it was prevalent in female non-smokers. PMID: 23246108
    47. polymorphisms in rs1047768 C/T and rs2296147 C/T are associated with response to platinum-based chemotherapy in advanced non-small-cell lung cancer, and XPG polymorphisms could be predictive of prognosis. PMID: 23621222
    48. Homozygoty for the wild-type Asp1104 SNP of the ERCC5 gene was found in 2 cases of relapsed osteosarcoma, who responded to trabectedin. PMID: 24192772
    49. XPG Asp1104His polymorphism was not associated with bladder cancer risk. PMID: 24061640
    50. XPG polymorphisms are associated with response to chemotherapy in osteosarcoma. PMID: 23886164

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  • 相关疾病：
    Xeroderma pigmentosum complementation group G (XP-G); Cerebro-oculo-facio-skeletal syndrome 3 (COFS3)
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    XPG/RAD2 endonuclease family, XPG subfamily
  • 数据库链接：

    HGNC: 3437

    OMIM: 133530

    KEGG: hsa:2073

    STRING: 9606.ENSP00000347978

    UniGene: Hs.258429