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  4. Recombinant Human Deoxyribonuclease gamma (DNASE1L3)

Recombinant Human Deoxyribonuclease gamma (DNASE1L3)

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  • 中文名称：
    人DNASE1L3重组蛋白
  • 货号：
    CSB-EP621686HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP621686HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DNASE1L3.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP621686HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DNASE1L3.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Deoxyribonuclease gamma; Deoxyribonuclease I like 3; Deoxyribonuclease I like III; Deoxyribonuclease I-like 3; DHP 2; DHP2; DNAS1L3; DNase gamma; DNase I homolog protein 2; DNase I homolog protein DHP2; DNase I like 3; DNase I-like 3; DNASE1L3; DNSL3_HUMAN; Liver and spleen DNase; LS DNase; LS-DNase; LSD; SLEB 16; SLEB16
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    60.4kDa
  • 表达区域：
    21-305aa
  • 氨基酸序列
    MRICSFNVRSFGESKQEDKNAMDVIVKVIKRCDIILVMEIKDSNNRICPILMEKLNRNSRRGITYNYVISSRLGRNTYKEQYAFLYKEKLVSVKRSYHYHDYQDGDADVFSREPFVVWFQSPHTAVKDFVIIPLHTTPETSVKEIDELVEVYTDVKHRWKAENFIFMGDFNAGCSYVPKKAWKNIRLRTDPRFVWLIGDQEDTTVKKSTNCAYDRIVLRGQEIVSSVVPKSNSVFDFQKAYKLTEEEALDVSDHFPVEFKLQSSRAFTNSKKSVTLRKKTKSKRS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
        脱氧核糖核酸酶gamma (DNASE1L3)能够同时切割单链和双链DNA，产生含有5'-磷酸基团和3'-羟基端的单脱氧核苷酸和寡脱氧核苷酸。在人体中，DNASE1L3主要参与到细胞的内部机制中，包括细胞凋亡和坏死过程中的DNA片段切割4。此外，它还能在细胞培养过程中作为添加物来消化细胞间的粘连，在细胞凋亡TUNEL检测中部分剪切基因组DNA作为阳性对照等。
        在科研领域，DNASE1L3也是一个重要的研究对象。由于其在DNA切割方面的特性，使得它在许多分子生物学和遗传学的实验中得到了广泛的应用。例如，在RNA提取或反转录前去除gDNA，或者在rRNA去除中消化DNA探针，缺口平移进行DNA标记，足迹法分析DNA-蛋白质相互作用，生成随机的DNA文库，减少细胞裂解物或蛋白提取物的粘性等。
        j9九游会登录入口首页生物大肠杆菌表达系统表达了全长重组人脱氧核糖核酸酶gamma (DNASE1L3)，助您进行相关研究。
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
  • 基因功能参考文献：
    1. The s found a significant reduction in serum DNase1l3 level in dermatomyositis/polymyositis and systemic lupus erythematosus, which may associate with clinic features and disease activity. PMID: 28039554
    2. Data indicate a likely functional variant influencing scleroderma susceptibility at deoxyribonuclease I-like 3 protein DNASE1L3 missense polymorphism rs35677470. PMID: 25332064
    3. Single nucleotide polymorphisms in the DNASE1L3 is associated with autoimmune diseases. PMID: 24206041
    4. DNASE1L3 mutations occur in hypocomplementemic urticarial vasculitis syndrome. PMID: 23666765
    5. We identified a rare autosomal recessive form of systemic lupus erythematosus, in which autozygome analysis revealed a null mutation in the DNASE1L3 gene PMID: 22019780
    6. investigates the distribution of DNASE1L3 gene SNPs in exons of the gene in eight Asian, three African, and three Caucasian populations worldwide using newly devised genotyping methods. PMID: 21692081
    7. The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells. PMID: 12154052
    8. Identification of two functional nuclear localization signals in DNase gamma that have a role in apoptotic DNA fragmentation. PMID: 12943533
    9. DNase gamma is involved in the generation of resected double-strand DNA breaks associated with somatic hypermutation PMID: 15629432
    10. DNAS1L3 plays an active role in lymphoma cell sensitization to VP-16 and its deficiency may constitute a novel mechanism of drug resistance in these cells PMID: 16427601
    11. A Caucasian-specific allele in SNP R206C produces an inactive form of DNase Il3. PMID: 19559017
    12. Results distinguish DNase gamma-dependent and CAD/DFF40-dependent DNA fragmentations, and show that even if DNA fragmentation is initiated by CAD/DFF40, DNase gamma is required for the more complete digestion of the genomic DNA in dying cells. PMID: 19574717

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  • 相关疾病：
    Systemic lupus erythematosus 16 (SLEB16)
  • 亚细胞定位：
    Nucleus. Endoplasmic reticulum. Secreted.
  • 蛋白家族：
    DNase I family
  • 组织特异性：
    Liver and spleen.
  • 数据库链接：

    HGNC: 2959

    OMIM: 602244

    KEGG: hsa:1776

    STRING: 9606.ENSP00000316193

    UniGene: Hs.476453