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  4. Recombinant Human Dimethylaniline monooxygenase [N-oxide-forming] 3 (FMO3)

Recombinant Human Dimethylaniline monooxygenase [N-oxide-forming] 3 (FMO3)

  • 中文名称：
    人FMO3重组蛋白
  • 货号：
    CSB-YP008749HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人FMO3重组蛋白
  • 货号：
    CSB-EP008749HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人FMO3重组蛋白
  • 货号：
    CSB-EP008749HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人FMO3重组蛋白
  • 货号：
    CSB-BP008749HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人FMO3重组蛋白
  • 货号：
    CSB-MP008749HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FMO3
  • Uniprot No.：
  • 别名：
    Dimethylaniline monooxygenase [N oxide forming] 3; Dimethylaniline monooxygenase [N-oxide-forming] 3; Dimethylaniline monooxygenase 3; Dimethylaniline oxidase 3; dJ127D3.1; Flavin containing monooxygenase 3; FMO 3; FMO form 2; FMO II; FMO3; FMO3_HUMAN; FMOII; Hepatic flavin containing monooxygenase 3; Hepatic flavin-containing monooxygenase 3; MGC34400; TMAU; Trimethylamine monooxygenase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-532
  • 氨基酸序列
    GKKVAIIGA GVSGLASIRS CLEEGLEPTC FEKSNDIGGL WKFSDHAEEG RASIYKSVFS NSSKEMMCFP DFPFPDDFPN FMHNSKIQEY IIAFAKEKNL LKYIQFKTFV SSVNKHPDFA TTGQWDVTTE RDGKKESAVF DAVMVCSGHH VYPNLPKESF PGLNHFKGKC FHSRDYKEPG VFNGKRVLVV GLGNSGCDIA TELSRTAEQV MISSRSGSWV MSRVWDNGYP WDMLLVTRFG TFLKNNLPTA ISDWLYVKQM NARFKHENYG LMPLNGVLRK EPVFNDELPA SILCGIVSVK PNVKEFTETS AIFEDGTIFE GIDCVIFATG YSFAYPFLDE SIIKSRNNEI ILFKGVFPPL LEKSTIAVIG FVQSLGAAIP TVDLQSRWAA QVIKGTCTLP SMEDMMNDIN EKMEKKRKWF GKSETIQTDY IVYMDELSSF IGAKPNIPWL FLTDPKLAME VYFGPCSPYQ FRLVGPGQWP GARNAILTQW DRSLKPMQTR VVGRLQKPCF FFHWLKLFAI PILLIAVFLV LT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential hepatic enzyme that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including drugs as well as dietary compounds. Plays an important role in the metabolism of trimethylamine (TMA), via the production of trimethylamine N-oxide (TMAO) metabolite. TMA is generated by the action of gut microbiota using dietary precursors such as choline, choline containing compounds, betaine or L-carnitine. By regulating TMAO concentration, FMO3 directly impacts both platelet responsiveness and rate of thrombus formation.
  • 基因功能参考文献：
    1. Finally, understanding the binding mode of methimazole and indole could be advantageous for development of hFMO3 inhibitors, currently investigated as a possible treatment strategy for atherosclerosis PMID: 29959003
    2. Report developmental regulation of hepatic FMO3 expression. PMID: 26839369
    3. the involvement of flavin-containing monooxygenase-3 (FMO3) and cytochrome P450 enzymes (CYPs) in Busulphan metabolic pathway, is reported. PMID: 29121650
    4. FMO3 protein abundance is significantly associated with age, gender, and genotype PMID: 28819071
    5. FMO3 Variant is associated with chronic kidney disease. PMID: 27513517
    6. The two FMO3 mutants were in close linkage disequilibrium and might play an important role in the pharmacokinetics of sulindac in Chinese healthy male volunteers. PMID: 28331852
    7. mechanism of hFMO3 which could be valuable not only for screening of new chemical entities but more significantly for designing of novel inhibitors of this important Phase I drug metabolising enzyme. PMID: 27523475
    8. Olanzapine clearance was not affected by CYP2D6 or FMO3 genotypes or smoking behavior as a single factor under the present conditions because olanzapine clearance is mediated by multiple enzymes involved in two major and one minor pathways PMID: 26856397
    9. Oxidative stress-responsive transcription factor NRF2 is not indispensable for the human hepatic Flavin-containing monooxygenase-3 (FMO3) gene expression in HepG2 cells PMID: 26616280
    10. FMO3 gene polymorphism E158K is a significant predictor of predisposition to chronic heart disease in women. PMID: 26519273
    11. FMO3 is centrally involved in the pathogenesis of atherosclerosis by regulating cholesterol metabolism and insulin resistance. [Review] PMID: 26218418
    12. FMO3 expression is increased in obese/insulin resistant patients. PMID: 25849138
    13. These results suggest that genetic polymorphism in the human FMO3 gene may lead to changes of drug interactions for N- or S-oxygenations of xenobiotics and endogenous substances. PMID: 25760532
    14. Effect of two-linked mutations of the FMO3 gene on itopride metabolism in Chinese healthy volunteers PMID: 25224784
    15. Three polymorphisms were found in intronic regions of FMO3 in a child with trimethylaminuria and in her parents and a brother. The parents and brother showed no symptoms. PMID: 25288227
    16. Comparisons of genotype and phenotype reveal that severe trimethylaminuria is caused by loss of function mutations in FMO3 PMID: 24028545
    17. results show that FMO3 E158K and POR A503V polymorphisms are minor sources of nicotine metabolism variation, insufficient to appreciably alter smoking. PMID: 24448396
    18. CYP3A4 and FMO3 are the major enzymes responsible for the metabolism of teneligliptin in humans. PMID: 23855261
    19. FMO3 (rs2266780) was in complete linkage disequilibrium with FMO6. PMID: 24173915
    20. single-nucleotide polymorphisms in the FMO3 gene is associated with chronic allograft dysfunction. PMID: 23350966
    21. Novel variations in the FMO3 gene associated with trimethylaminuria. PMID: 23791655
    22. provides fundamental, up-to-date information on the importance of human FMO3 in individual xenobiotic oxygenations, including those of new medicines and dietary-derived trimethylamine PMID: 23567996
    23. FMO3 heterozygote genotypes increase the risk of stroke 6 times in hypertensives in Turkish population. PMID: 23510775
    24. Polymorphisms in FMO3 influence nicotine clearance and that these genetic variants in turn influence cigarette consumption. PMID: 23211429
    25. Genetic variations in the FMO3 gene in patients with trimethylaminuria do not always present with fish-like body odor. PMID: 23266626
    26. Six novel variants of the FMO3 gene have been found responsible for trimethylaminuria in a Japanese population. PMID: 22819296
    27. It was found that FMO3 158K, 257M and 308G alleles, demonstrate impaired metabolism toward many FMO3 substrates, were observed frequently in Turkish population similar to the other populations PMID: 22409263
    28. patient heterozygous for 4 FMO3 mutations PMID: 21075259
    29. This study is the first to demonstrate a gene-environment interaction in SIDS. The findings suggest that the common polymorphism G472A of FMO3 could act as an additional genetic SIDS risk factor in children whose mothers smoke. PMID: 20198379
    30. These data suggest that birth is necessary, but not sufficient for the onset of FMO3 expression. A gender-independent increase in FMO3 expression was observed from 11 to 18 y of age. PMID: 11809920
    31. two new polymorphisms, His132-FMO3 and Pro360-FMO3, were identified; the effect of the amino acid substitutions on the function of FMO3 was evaluated PMID: 12814961
    32. Data show that the frequencies of FMO3 mutant alleles varied not only in different ethnic groups, but also in different populations that stemmed from the same ethnic group. PMID: 12903042
    33. ..FMO3 appears to be the most important flavin-containing monoxygenase..in adult human liver. p. 574 ..human FMO3 appears to oxygenate nucleophilic heteroatom-containing substrates.." p. 576 PMID: 15203093
    34. FMO3 displayed a significant, dominant liver-specific mRNA profile. PMID: 16183778
    35. variants in the FMO3 gene do not predispose to essential hypertension in a cardiovascular disease population of 1649 individuals, 691(41.9%) of whom had a history of hypertension requiring drug treatment PMID: 16324215
    36. Flavin-containing monooxygenase 3 (FMO3) is responsible for the majority of FMO-mediated xenobiotic metabolism in the adult human liver. PMID: 16481213
    37. Genetic basis of trimethylaminuria is studied in Italian pedigrees and shows a spectrum of variation in the FM03 gene with 3 novel mutations: a de novo missense mutation, a deletion (G1182del) at codon 394 and a novel missense mutation (R238P). PMID: 16600650
    38. The genotypes and allele frequencies of the MDR1/C3435T, FMO3/G488A, FMO3/A923G and CYP1A2/G-3860 A polymorphisms were not significantly different in cancer-free subjects and CRC patients. PMID: 16800822
    39. The study is said to be the first report for significant single nucleotide polymorphisms of the FMO3 causing amino acid substitutions in Japanese trimethylaminuria patients. PMID: 16858129
    40. As summarized in this review, FMO3 is apparently unique to the human, yet is the most abundant FMO family member in the adult human liver, whereas FMO1 dominates in most animal models. PMID: 16863467
    41. Individuals homozygous for either of the nonsense mutations--Arg500Stop and/or Cys197Stop--alleles in the FMO3 gene can possess abnormal trimethylamine N-oxygenation. PMID: 16996766
    42. results suggest that the effects of genetic variation of FMO3 could operate at the functional level for N- & S-oxygenation for typical FMO3 substrates PMID: 17142560
    43. abnormal FMO3 capacity is caused by menstruation particularly in the presence, in homozygous form, of mild genetic variants such as [Glu158Lys; Glu308Gly] that cause a reduced FMO3 function PMID: 17257434
    44. A homology model for FMO3 was constructed based on the crystal structure for yeast FMO which places the N61 residue alone, of the mutants analyzed here, in close proximity to the FAD catalytic center. PMID: 17531949
    45. This review summarizes the current state of research on the genetic polymorphisms of FMO3, with a focus on their clinical implications in gastrointestinal diseases. PMID: 17559352
    46. Mutations of the FMO3 gene were investigated in Japanese trimethylaminuria that showed low FMO3 metabolic capacity. PMID: 17584019
    47. FMO3 is the primary human adult liver FMO enzyme, but is developmentally regulated PMID: 17786630
    48. TG100435 and TG100855 were interconverted metabolically. FMO3 seem to be the major N-oxidizing enzyme, whereas cytochrome P450 reductase seems to be responsible for the retroreduction reaction. PMID: 17881660
    49. The results provide evidence that FMO3 has been the subject of balancing selection. PMID: 17885620
    50. Site-directed mutagenesis studies suggest that the putative hepatic nuclear factor-4 (HNF-4) binding site and CCAAT box could be responsible cis-acting elements of the FMO3 gene in a Japanese population. PMID: 18305374

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  • 相关疾病：
    Trimethylaminuria (TMAU)
  • 亚细胞定位：
    Microsome membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    FMO family
  • 组织特异性：
    Liver.
  • 数据库链接：

    HGNC: 3771

    OMIM: 136132

    KEGG: hsa:2328

    STRING: 9606.ENSP00000356729

    UniGene: Hs.445350