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  4. Recombinant Human Dual specificity protein phosphatase 6 (DUSP6)

Recombinant Human Dual specificity protein phosphatase 6 (DUSP6)

  • 货号：
    CSB-YP621973HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP621973HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP621973HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP621973HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dual specificity phosphatase 6; Dual specificity phosphatase 6 isoform a; Dual specificity protein phosphatase 6; Dual specificity protein phosphatase PYST1; DUS6_HUMAN; DUSP 6; DUSP 6a; Dusp6; DUSP6a; HH19; MAP kinase phosphatase 3; Mitogen activated protein kinase phosphatase 3; Mitogen-activated protein kinase phosphatase 3; MKP 3; MKP-3; MKP3; PYST 1; PYST1; Serine/threonine specific protein phosphatase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-381
  • 氨基酸序列
    MIDTLRPVPF ASEMAISKTV AWLNEQLELG NERLLLMDCR PQELYESSHI ESAINVAIPG IMLRRLQKGN LPVRALFTRG EDRDRFTRRC GTDTVVLYDE SSSDWNENTG GESVLGLLLK KLKDEGCRAF YLEGGFSKFQ AEFSLHCETN LDGSCSSSSP PLPVLGLGGL RISSDSSSDI ESDLDRDPNS ATDSDGSPLS NSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT PNLPNLFENA GEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS VTVTVAYLMQ KLNLSMNDAY DIVKMKKSNI SPNFNFMGQL LDFERTLGLS SPCDNRVPAQ QLYFTTPSNQ NVYQVDSLQS T
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain. Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors.
  • 基因功能参考文献：
    1. Individuals are carrying DUSP6 rs2279574 AA and AC genotypes associated with an increased risk in developing lung squamous carcinoma in Han Chinese and with advanced NSCLC stages. PMID: 29578153
    2. DUSP6 expression in skeletal muscle was reduced by 43% just after exercise and remained below pre-exercise level after 2 h recovery. PMID: 28989118
    3. Data show that oxidative stress and MAP kinase phosphatase 3 (MKP3) inhibition play a critical role in procyanidin B2 3,3''-di-O-gallate (B2G2)-induced cell death in prostate cancer (PCa) cells through sustained activation of both ERK1/2 and AMPKalpha. PMID: 28876465
    4. High DUSP6 expression is associated with Lung Squamous Cell Carcinoma. PMID: 27613525
    5. s demonstrate the broad applicability of this recombination-based method and they proved its potential to identify new drug targets via the identification of the tumor suppressor DUSP6 as potential synthetic lethal target in melanoma cell lines with BRAF V600E mutations and high DUSP6 expression. PMID: 28423600
    6. PICSAR has a role in promoting cSCC progression via activation of extracellular signal-regulated kinase 1/2 signaling pathway by downregulating DUSP6 expression PMID: 27049681
    7. DUSP6 rs2279574 SNPs was not associated with chemoradiotherapy response, whereas tumor regression, weight loss, clinical stage, and cigarette smoking were independent prognostic predictors for these Chinese patients with non-small cell lung cancer. PMID: 28231233
    8. DNA samples from each patient were genotyped for DUSP6 and TOP2A SNPs PMID: 27156317
    9. Suppression of the FOXA1/DUSP6 signaling pathway may contribute to the development of Hirschsprung disease. PMID: 26097584
    10. These observations led us to conclude that increased TSH signaling overcomes OIS and is essential for B-RafV600E-induced papillary thyroid carcinogenesis. PMID: 25499223
    11. Dusp6 expression was higher in progestin-sensitive atypical endometrial hyperplasia groups compared with progestin-resistant groups. After treatment, Dusp6 expression was upregulated in progestin-sensitive groups, but not in progestin-resistant groups. PMID: 25451692
    12. Our results suggest that DUSP6 gene expression in tumour samples may be a prognostic marker in NSCLC PMID: 25344212
    13. DUSP6 downregulated the expression of matrix metallopeptidase 3. PMID: 25241655
    14. These results therefore indicate that p53-mediated up-regulation of MKP-3 contributes to the establishment of the senescent cellular phenotype through dephosphorylating ERK1/2 PMID: 25414256
    15. Downregulation of Dusp6, Sprouty4, and Sef--negative modulators of FGF2/ERK1/2 signaling--was present in eutopic endometria of adenomyosis, which may play critical roles in the development of adenomyosis. PMID: 24681741
    16. Higher expression of DUSP6 in tumor tissue, than in peritumor tissue, is associated with the recurrence after curative resection of HCC, and the relative tumor DUSP6 expression has good power to predict the recurrence of HCC. PMID: 24709168
    17. suggest an important role for DeltaNp63alpha in preventing cancer metastasis by inhibition of Erk2 signaling via MKP3 subscribing PMID: 23246965
    18. Dusp6 overexpression is involved in the pathogenesis and development of human endometrial adenocarcinoma. PMID: 23419500
    19. Increased DUSP6 expression is associated with papillary thyroid carcinoma. PMID: 24222120
    20. Depletion of DUSP6 reduced the viability of cancer cell lines and increased the cytotoxicity of EGFR and other targeted inhibitors, and cytotoxic agents. PMID: 23839489
    21. missense mutation, c.545C>T (p.Ser182Phe), in the DUSP6 gene may be genetically linked to the Class III malocclusion PMID: 23965468
    22. DUSP6 is overexpressed in papillary and poorly differentiated thyroid carcinoma and contributes to neoplastic properties of thyroid cancer cells. PMID: 23132790
    23. The results provide insights on the modulatory role of p53 in the survival pathway by up-regulating DUSP6. PMID: 23108049
    24. study reports that naive CD4 T cells from elderly individuals have reduced signaling capacity of the ERK pathway; the underlying mechanism is an age-related decline in miR-181a expression and associated rise in levels of DUSP6 expression PMID: 23023500
    25. ischemia/reperfusion inhibited eNOS expression by inactivation of ERK1/2, leading to decreased NO formation through a MKP-3-dependent mechanism in endothelial cells PMID: 22848708
    26. DUSP6 plays an important role in the pathogenesis of bipolar disorder, particularly in women. PMID: 22155192
    27. MESP2, HES7 and DUSP6 genes may not be involved in the etiopathogenesis of sporadic and non-syndromic CS in Chinese Han population. PMID: 22744456
    28. A link between DUSP6 expression and high-risk features of papillary thyroid carcinoma suggested that DUSP6 is an important independent factor affecting the signaling pathways in established papillary thyroid carcinoma. PMID: 22535643
    29. DUSP6 is important in melanoma and it plays a different role in the distinct subtype of mouse melanoma compared with that in classic human melanoma. PMID: 22171919
    30. caspase-3 cleavage of MKP3 down-regulates MKP3 full length and renders active MKP3 fragments, which may participate in novel regulatory pathways controlling the subcellular localization and activation of ERK1/2 during apoptosis. PMID: 22504224
    31. Studies identified a single gene DUSP6, whose expression was associated with sensitivity to GSK1120212. PMID: 22169769
    32. Studies indicate that ERKs stimulate their own dephosphorylation by directly binding to phosphatases, such as MKP-3, and activating them. PMID: 22028468
    33. Loss of DUSP6 is associated with esophageal squamous cell carcinoma and nasopharyngeal carcinoma. PMID: 21387288
    34. DUSP6 plays an important role in cancer resistance in different subtypes of non-small cell lung carcinoma. PMID: 21680106
    35. upregulation of DUSP6 exerts a tumor-promoting role in human glioblastomas exacerbating the malignant phenotype. PMID: 21499306
    36. MKP3 not only controls the activities of ERK2 and p38alpha but also mediates cross-talk between these two MAPK pathways. PMID: 21454500
    37. This study shows that post-transcriptional regulation is a key process in the control of DUSP6 expression. PMID: 20665674
    38. DUSP6 methylation is a rare event in endometrial cancer. Silencing of the DUSP6 phosphatase is unlikely to contribute to constitutive activation of the ERK kinase cascade in endometrial cancer. PMID: 20638106
    39. Dual specificity phosphatase 6 (DUSP6) is an ETS-regulated negative feedback mediator of oncogenic ERK signaling in lung cancer cells. PMID: 20097731
    40. Angiotensin II-induced upregulation of MAP kinase phosphatase-3 mRNA levels mediates endothelial cell apoptosis PMID: 11998972
    41. Results show that DUSP6 exerts apparent tumor-suppressive effects in vitro and suggest that DUSP6 is a strong candidate tumor suppressor gene at 12q22 locus. PMID: 12759238
    42. Constitutive induction of p-Erk1/2 accompanied by reduced activities of protein phosphatases 1 and 2A and MKP3 due to reactive oxygen species during cellular senescence. PMID: 12840032
    43. Study gives first direct evidence that the N-terminal domain of MKP3 mediates intramolecular dephosphorylation between the monomeric form of phosphorylated extracellular signal-regulated kinase and a monomer of MKP3. PMID: 14690430
    44. cytoplasmic localization of MKP3 is mediated by a chromosome region maintenance-1 (CRM1)-dependent nuclear export pathway; the ability of MKP3 to cause the cytoplasmic retention of ERK2 requires both a functional kinase interaction motif and NES PMID: 15269220
    45. Hypermethylation with modification of histone deacetylation play an important role in transcriptional suppression of DUSP6 in human pancreatic cancer PMID: 15824892
    46. Results suggest that the abrogation of DUSP6 is associated exclusively with progression from pancreatic intraepithelial neoplasia to the invasive ductal carcinoma. PMID: 15832194
    47. Results demonstrate that in the Korean population DUSP6 may contribute to the etiology of bipolar disorder, but not schizophrenia. PMID: 16491131
    48. Nuclear matrix proteins such as mutant Pyst1 and nucleophosmin 1 were downregulated, whereas eIF6 and beta-tubulin were upregulated during cell differentiation in hepatocarcinoma cells. PMID: 17569113
    49. even upon over-expression DUSP6 fails to inactivate ERK5, confirming that it is indeed an ERK1/2-specific DUSP PMID: 18280112
    50. MKP3 can act to enhance DAT function and is a phosphatase involved in regulating dynamin-dependent endocysis PMID: 18434601

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  • 相关疾病：
    Hypogonadotropic hypogonadism 19 with or without anosmia (HH19)
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
  • 组织特异性：
    Expressed in keratinocytes (at protein level).
  • 数据库链接：

    HGNC: 3072

    OMIM: 602748

    KEGG: hsa:1848

    STRING: 9606.ENSP00000279488

    UniGene: Hs.298654