Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A)

Recombinant Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A)

  • 货号：
    CSB-YP614437HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP614437HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP614437HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP614437HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP614437HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dual specificity tyrosine phosphorylation regulated kinase; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A; Dual specificity tyrosine phosphorylation regulated kinase 1; Dual specificity tyrosine phosphorylation regulated kinase 1A; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual specificity YAK 1 related kinase; Dual specificity YAK1 related kinase; Dual specificity YAK1-related kinase; DYR1A_HUMAN; DYRK 1; DYRK 1A; DYRK; DYRK1; Dyrk1a; DYRKA; hMNB; HP 86; HP86; Minibrain (Drosophila) homolog; Minibrain homolog; Minibrain; Drosophila; homolog of; MNB; MNB protein kinase; Mnb protein kinase homolog hp86; MNB protein kinase; Serine/threonine-specific; MNB/DYRK protein kinase; MNBH; MRD7; OTTHUMP00000109090; OTTHUMP00000109091; OTTHUMP00000109094; OTTHUMP00000174799; Protein kinase minibrain homolog; Serine/threonine kinase MNB
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-763
  • 氨基酸序列
    MHTGGETSAC KPSSVRLAPS FSFHAAGLQM AGQMPHSHQY SDRRQPNISD QQVSALSYSD QIQQPLTNQV MPDIVMLQRR MPQTFRDPAT APLRKLSVDL IKTYKHINEV YYAKKKRRHQ QGQGDDSSHK KERKVYNDGY DDDNYDYIVK NGEKWMDRYE IDSLIGKGSF GQVVKAYDRV EQEWVAIKII KNKKAFLNQA QIEVRLLELM NKHDTEMKYY IVHLKRHFMF RNHLCLVFEM LSYNLYDLLR NTNFRGVSLN LTRKFAQQMC TALLFLATPE LSIIHCDLKP ENILLCNPKR SAIKIVDFGS SCQLGQRIYQ YIQSRFYRSP EVLLGMPYDL AIDMWSLGCI LVEMHTGEPL FSGANEVDQM NKIVEVLGIP PAHILDQAPK ARKFFEKLPD GTWNLKKTKD GKREYKPPGT RKLHNILGVE TGGPGGRRAG ESGHTVADYL KFKDLILRML DYDPKTRIQP YYALQHSFFK KTADEGTNTS NSVSTSPAME QSQSSGTTSS TSSSSGGSSG TSNSGRARSD PTHQHRHSGG HFTAAVQAMD CETHSPQVRQ QFPAPLGWSG TEAPTQVTVE THPVQETTFH VAPQQNALHH HHGNSSHHHH HHHHHHHHHG QQALGNRTRP RVYNSPTNSS STQDSMEVGH SHHSMTSLSS STTSSSTSSS STGNQGNQAY QNRPVAANTL DFGQNGAMDV NLTVYSNPRQ ETGIAGHPTY QFSANTGPAH YMTEGHLTMR QGADREESPM TGVCVQQSPV ASS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6. Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis.
  • 基因功能参考文献：
    1. This study provides evidence for an essential role of DYRK1A as balanced regulator of S-phase entry in Hepatic progenitor cells (HPCs). An exact gene dosage is crucial, as both DYRK1A deficiency and overexpression affect HPC cell cycle progression. PMID: 29179659
    2. LncRNA OIP5-AS1 suppressed cell viability, promoted radio-induced apoptosis, and enhanced the radiosensitivity of CRC cells by regulating DYRK1A expression through miR-369-3p. PMID: 29773344
    3. The s show here that chemical inhibition or genetic knockdown of DYRK1A interferes with neural specification of human pluripotent stem cells, a process equating to the earliest stage of human brain development. Specifically, DYRK1A inhibition insulates the self-renewing subpopulation of human pluripotent stem cells from powerful signals that drive neural induction. PMID: 28884684
    4. These results indicate a functional deficiency of DYRK1A as an underlying disease mechanism for autism. PMID: 29021890
    5. Inhibition of DYRK1A resulted in an increased apoptosis and decrease in invasion and colony formation ability of HNSCC cell lines. PMID: 27796319
    6. Immunoprecipitation and pulldown experiments identified DCAF7 as an adaptor for the association of the adenovirus E1A protein with DYRK1A and HIPK2 PMID: 27307198
    7. Results associate a decreased level of DYRK1A with Alzheimer's disease and challenge the use of DYRK1A inhibitors in peripheral tissues as treatment. PMID: 28632203
    8. The locations of disruptive variants (truncating, missense, and splice site mutations), copy number variations, and chromosomal rearrangements affecting DYRK1A functions. DYRK1A is one of the most recurrent genes with disruptive single nucleotide variants implicated in Autism spectrum disorder. PMID: 29034068
    9. we suggest that de novo dominant mutations in DYRK1A account for nearly 0.5% of severe developmental disorders due to substantially reduced kinase function PMID: 28053047
    10. The data described herein provide the first identification of a DYRK1A-mediated site of phosphorylation on GLI1 within its NLS and may serve as a valuable mechanism for further understanding Hh signaling modulation. PMID: 28735864
    11. DYRK1A phosphorylation of NFATc1/alphaA at S261, S278, S403 and S409 interfered with NFATc1 ubiquitination and ubiquitin-proteasome degradation. PMID: 28235034
    12. These results suggest that TT genotype derived from SNP rs8126696 of DYRK1A gene is a possible risk factor for sporadic Parkinson disease, especially for males in this Chinese Han population. PMID: 27546826
    13. identified the significant associations of the GBA L444P mutation and DYRK1A rs8126696 T allele with the earlier age at onset (AAO) in Parkinson's disease (PD) patients, and the A allele at MS4A6A rs610932 with the delayed AAO of PD. PMID: 27085534
    14. study uncovered a new regulatory mechanism of DYRK1A degradation by SCF(betaTrCP) in HEK293 cell cycle progression. PMID: 27807027
    15. Identify Dyrk1a as a novel negative regulator of D-cyclin-mediated Rb1/E2f-signalling. As dysregulation of this pathway with impaired cardiomyocyte proliferation leads to cardiomyopathy. PMID: 27056896
    16. The deleterious effect of DYRK1A triplication in the formation of the cerebral cortex begins at the onset of neurogenesis, which is relevant to the search for early therapeutic interventions in Down syndrome. PMID: 26137553
    17. mutations in DYRK1A define a syndromic form of autism spectrum disorder and intellectual disability with neurodevelopmental defects consistent with murine and Drosophila knockout models PMID: 25707398
    18. Cyclin D1 Again Caught in the Act: Dyrk1a Links G1 and Neurogenesis in Down Syndrome. PMID: 26137545
    19. data indicate that host factor DYRK1A plays a role in the regulation of viral transcription and latency. PMID: 26641855
    20. Inhibition of DYRK1A and GSK3B induces human beta-cell proliferation PMID: 26496802
    21. A de novo splice site mutation and a de novo nonsense mutation in DYRK1A were identified in two patients with syndromic intellectual disability. PMID: 26922654
    22. RNA-seq evidence of biallelic expression of DYRK1A and 10 neighboring genes in at least one primary human tissue tested indicates that the expression of DYRK1A is uncoupled from the control of the maternally inherited 5mCpG imprints at the WRB differentially methylated region (DMR) in disomic controls or trisomy (Down syndrome) individuals. PMID: 27100087
    23. Our report represents the largest cohort of individuals with DYRK1A disruptions to date, and is the first attempt to define consistent genotype-phenotype correlations among subjects with 21q22.13 microdeletions and DYRK1A SNVs or small INDELs. PMID: 25944381
    24. We identified unique truncating and non-synonymous mutations (three nonsense, four frameshift and two missense) in DYRK1A in nine patients and a large chromosomal deletion that encompassed DYRK1A in one patient PMID: 25920557
    25. the increased immunostaining of DYRK1A in HIV+ brains without pathology points at dysregulation of DYRK1A as an early event in the neuronal complications of HIV infection. PMID: 26534959
    26. novel mutations in DYRK1A contribute to the phenotype of the DYRK1A-associated developmental disorder PMID: 25641759
    27. findings suggest that truncation/activation of Dyrk1A by Ca(2+)/calpain I might contribute to Tau pathology via promotion of exon 10 exclusion and hyperphosphorylation of Tau in AD brain PMID: 25918155
    28. Then we used this method to analyse human plasma DYRK1A levels in patients with AD biologically confirmed (positive CSF biomarkers and PiB-PET) and controls defined by normal cognitive performances and negative PiB-PET imaging. PMID: 25116835
    29. Tumor suppressor DYRK1A effects on proliferation and chemoresistance of AML cells by downregulating c-Myc. PMID: 24901999
    30. Results show that DYRK1A associates with the promoters of genes actively transcribed by RNAPII, where it regulates transcription by locally phosphorylating CTD residues involved in transcriptional elongation. PMID: 25620562
    31. Study shows that DYRK1A overexpression affects pathways involved in synaptogenesis and synaptic plasticity and influences excitation/inhibition balance toward inhibition PMID: 24801365
    32. results demonstrate that the molecular chaperone TRiC/CCT is essential for correct protein folding, DYRK1A binding, and nuclear accumulation of WDR68. PMID: 25342745
    33. Overexpression of DYRK1A may promote premature neuronal differentiation and contribute to altered brain development in Down syndrome. PMID: 24806449
    34. DYRK1A phoshorylates histone H3 to differentially regulate the binding of HP1 isoforms and antagonize HP1-mediated transcriptional repression. PMID: 24820035
    35. This review suggested that DYRK1A overexpressure in brain is releate to Down syndrome. PMID: 24152332
    36. This study supports the hypothesis that intracellular distribution and compartment-specific functions of DYRK1A may depend on its phosphorylation pattern. PMID: 24327345
    37. Dyrk1A does not enhance tau gene transcription, but does increase tau mRNA stability. PMID: 23948904
    38. DYRK1A overexpression in a developmental alteration of the central nervous system associated with Down syndrome. PMID: 23512985
    39. Dosage of Dyrk1a repositions cells within a p21-CycD1 signaling map, directing each cell to either proliferate or to follow two distinct cell cycle exit pathways characterized by high or low CycD1 and p21 levels. PMID: 24119401
    40. A fundamental role for the dual-specificity tyrosine phosphorylation-regulated kinase, DYRK1A, in regulating EGF receptor in glioblastomas. PMID: 23635774
    41. The DYRK1A gene is a cause of syndromic intellectual disability with severe microcephaly and epilepsy. PMID: 23099646
    42. Coexpression of Dyrk1A and splicing factor 9G8 leads to their translocation from the nucleus to the cytoplasm and suppresses their ability to regulate tau exon 10 splicing. PMID: 21215488
    43. A negative correlation was detected between BDNF and DYRK1A levels in lymphoblastoid cell lines with complete aneuploidy of human chromosome 21. PMID: 22669612
    44. our results highlight the role played by DYRK1A in brain development through the control of choline acetyltransferase expression PMID: 23124096
    45. This study demonistrated that the Gene dosage-dependent association of DYRK1A with the cytoskeleton in the brain and lymphocytes in patient with down syndrome patients PMID: 23147510
    46. Mnb/Dyrk1a regulates food intake through the evolutionary conserved Sir2-FOXO-sNPF/NPY pathway in Drosophila melanogaster and mammals. PMID: 22876196
    47. Phosphorylation of SRp55 by Dyrk1A suppressed its ability to promote Tau exon 10 inclusion. PMID: 22767602
    48. This study showed Dyrk1A is shown to interact with and phosphorylate Munc18-1 at the Thr479 residue PMID: 22765017
    49. This study demonistrated that a significant association between a polymorphic marker for DYRK1A and alpha-synuclein dementias. PDD. PMID: 22269890
    50. analysis of phosphorylation regulated kinase 1A (DYRK1A) inhibitors PMID: 22154664

    显示更多

    收起更多

  • 相关疾病：
    Mental retardation, autosomal dominant 7 (MRD7)
  • 亚细胞定位：
    Nucleus. Nucleus speckle.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MNB/DYRK subfamily
  • 组织特异性：
    Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
  • 数据库链接：

    HGNC: 3091

    OMIM: 600855

    KEGG: hsa:1859

    STRING: 9606.ENSP00000381932

    UniGene: Hs.368240