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  4. Recombinant Human E3 SUMO-protein ligase PIAS3 (PIAS3)

Recombinant Human E3 SUMO-protein ligase PIAS3 (PIAS3)

  • 货号：
    CSB-YP896765HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP896765HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP896765HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP896765HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP896765HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    E3 SUMO protein ligase PIAS 3; E3 SUMO protein ligase PIAS3; E3 SUMO-protein ligase PIAS3; FLJ14651; OTTHUMP00000015586; OTTHUMP00000015587; PIAS 3; Pias3; PIAS3 protein; PIAS3_HUMAN; Protein inhibitor of activated STAT 3; Protein inhibitor of activated STAT protein 3; Protein inhibitor of activated STAT3; Zinc finger MIZ type containing 5; ZMIZ 5; ZMIZ5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-628
  • 氨基酸序列
    MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA PSVQMKIKEL YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL LAPGTLLGPK REVDMHPPLP QPVHPDVTMK PLPFYEVYGE LIRPTTLAST SSQRFEEAHF TFALTPQQVQ QILTSREVLP GAKCDYTIQV QLRFCLCETS CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR PINITPLARL SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD AALYLQMNEK KPTWTCPVCD KKAPYESLII DGLFMEILSS CSDCDEIQFM EDGSWCPMKP KKEASEVCPP PGYGLDGLQY SPVQGGDPSE NKKKVEVIDL TIESSSDEED LPPTKKHCSV TSAAIPALPG SKGVLTSGHQ PSSVLRSPAM GTLGGDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP GRVSSIVAPG GALREGHGGP LPSGPSLTGC RSDIISLD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus.
  • 基因功能参考文献：
    1. PAI-1 interacted with PIAS3 to regulate Stat3-dependent gene expression and miR-34a was transcriptionally suppressed by Stat3 to form a positive regulatory loop through Stat3 signaling in non-small cell lung cancer cells. PMID: 28988111
    2. Levels of PIAS3 are significantly lower, in contrast with phosphorylation of STAT3, in women with endometriosis compared to women without endometriosis. PMID: 27226311
    3. TRIM8 activates STAT3 by suppressing the expression of PIAS3, an inhibitor of STAT3, most likely through E3-mediated ubiquitination and proteasomal degradation. PMID: 28100038
    4. Low PIAS3 expression is associated with breast cancer organoid invasiveness. PMID: 28423498
    5. SHP2, SOCS3 and PIAS3 levels are reduced in medulloblastomas in vivo and in vitro, of which PIAS3 downregulation is more reversely correlated with STAT3 activation. In resveratrol-suppressed medulloblastoma cells with STAT3 downregulation and decreased incidence of STAT3 nuclear translocation, PIAS3 is upregulated, the SHP2 level remains unchanged and SOCS3 is downregulated. PMID: 28035977
    6. The results revealed that although the expression levels of SOCS1, SOCS3 and, in particular, pSHP2, tend to decrease in the four types of astrocytomas, PIAS3 downregulation is more negatively correlated with STAT3 activation in the stepwise progress of astrocytomas and would indicate an unfavorable outcome. PMID: 28035384
    7. 3-Formylchromone inhibits proliferation and induces apoptosis of multiple myeloma cells by abrogating STAT3 signaling through the induction of PIAS3. PMID: 27324722
    8. PIAS3 may serve as a biomarker for predicting hormone therapy stratification, although it is limited to those breast cancer patients receiving hormone therapy. PMID: 26768588
    9. Taken together, these results suggest that PIAS3 functions as a positive regulator of HIF-1alpha-mediated transcription by increasing its protein stability. PMID: 26697750
    10. PIAS3 suppression may be protective against joint destruction in rheumatoid arthritis by regulating synoviocyte migration, invasion, and activation. PMID: 26667168
    11. PIAS3 primes ATR for checkpoint activation. PMID: 26565033
    12. PIAS3 expression in squamous cell lung cancer is low and predicts overall survival PMID: 25573684
    13. Adenovirus E4-ORF3 targets PIAS3 and together with E1B-55K remodels SUMO2/SUMO3 Interactions in the nucleus and at virus genome replication domains. PMID: 26223632
    14. Statistically significant positive correlation has been found between STAT5B and COX-2, and significant negative correlation between STAT5B and PIAS3. PMID: 25137041
    15. Low PIAS3 expression in malignant mesothelioma is associated with increased STAT3 activation and poor patient survival. PMID: 25124686
    16. this newly identified p53PIAS3 interaction through the 1-52 amino acid region of p53, reduces p53MDM2 complex formation, which not only increases the half-life of p53, but also its transactivation of target genes. PMID: 24584189
    17. PIAS3 may be a potential biomarker target for early cancer detection and therapeutic of human colorectal cancer. PMID: 24120699
    18. PIAS3 activates the intrinsic apoptotic pathway in non-small cell lung cancer cells independent of p53 status. PMID: 23959540
    19. Downregulation of PIAS3 is associated with gastric adenocarcinogenesis. PMID: 23322197
    20. PIAS3 expression is closely related to the invasion properties of glioma TJ905 cells. PMID: 21733403
    21. NF-kappaB is regulated through a novel negative feedback mechanism by SUMOylation, where the RelA subunit of NF-kappaB is SUMOylated by PIAS3. PMID: 22649547
    22. PIAS3 is a novel regulator of ErbB4 receptor tyrosine kinase, controlling its nuclear sequestration and function. PMID: 22584572
    23. Squamous cell carcinoma of the lung commonly lacks PIAS3 protein expression. PMID: 21497567
    24. novel transcription factor binding partners for PIAS3 including ETS, EGR1, NR1I2, and GATA1 were identified. PMID: 21532337
    25. Identification of vimentin (vimentin354), a nuclear component in glioblastoma multiforme cells, as the main target of sumoylation promoted by PIAS3. PMID: 21317457
    26. SOCS-3 and PIAS-3 upregulation impairs IL-12-mediated interferon-gamma response in CD56 T cells in HCV-infected heroin users PMID: 20231901
    27. These findings indicate that TRIM8 enhances the STAT3-dependent signal pathway by inhibiting the function of PIAS3. PMID: 20516148
    28. we selected one coding single nucleotide polymorphism in PIAS3 for association analyses in patients with breast cancer PMID: 19760037
    29. there is an interaction between Zimp7 and PIAS proteins with higher preference for PIAS3, in androgen receptor-mediated transcription PMID: 20159969
    30. data suggest an important role for the negative regulatory effect of PIAS3 on STAT3 in EGF-driven tumors PMID: 19903771
    31. AR and PIAS3 regulate the STAT3-mediated transcriptional activity by their physical protein-protein competition on STAT3 PMID: 12804609
    32. Up-regulation of protein inhibitor of activated STAT3 protein is associated with adult T-cell leukemia PMID: 14630083
    33. PIAS3 and Smad3 interact with each other at the endogenous protein level in mammalian cells and also in vitro, and the association occurs through the C-terminal domain of Smad3. PMID: 14691252
    34. In conclusion, ATBF1 can suppress the IL-6-mediated cellular response by acting together with PIAS3. PMID: 14715251
    35. Increased PIAS3 expression is associated with human cancers including lung, breast, prostate, colon-rectum, and brain tumors PMID: 15138572
    36. PIAS3 may function in vivo as a modulator in suppressing the transcriptional activity of p65 PMID: 15140884
    37. End-stage hepatitis c and alcoholic liver cirrhosis is characterized by impaired Stat3 DNA-binding possibly through up-regulation of Pias3. PMID: 16098628
    38. Mechanisms of PIAS3 activity could pave the way toward the formulation of an antioncogenic agent for the inhibition of both STAT3 and MITF. PMID: 16368885
    39. overexpression of PIAS3 in lung cancer cells contributed to growth suppression and restored the drug sensitivity of the cells PMID: 17032498
    40. PIAS3 is a new regulator of ATF1 that regulates the ARE-mediated transcription of the ferritin H gene PMID: 17565989
    41. Both SUMO E2 conjugating enzyme Ubc9 and E3 ligase protein inhibitor of activated STAT3 (Pias3) are targets for S-nitrosation PMID: 17987106
    42. PIAS3 binds to Tel and stimulates sumoylation of K11 in the nucleus.PIAS3 may have a dual, context-dependent influence on Tel; it mediates Tel sumoylation, but it also augments Tel's repressive function in a sumoylation-independent fashion. PMID: 18212042
    43. parvovirus B19 NS1 protein modulates inflammatory signaling by activation of STAT3/PIAS3 in human endothelial cells PMID: 18550668
    44. This study suggests a link between PIAS3 and nuclear pyruvate kinase. PMID: 19308990
    45. PIAS3 is expressed in NSCLC cell lines and its over-expression decreased STAT3 transcriptional activity, decreased proliferation of NSCLC cells and when used in conjunction with EGFR inhibitors, increased the anti-proliferative effects. PMID: 19569236
    46. Pias3 associates and colocalizes with Trim32, a Ubiquitin E3 ligase. PMID: 17987106

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus speckle.
  • 蛋白家族：
    PIAS family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 16861

    OMIM: 605987

    KEGG: hsa:10401

    STRING: 9606.ENSP00000376765

    UniGene: Hs.435761