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  4. Recombinant Human E3 ubiquitin-protein ligase DTX3L (DTX3L)

Recombinant Human E3 ubiquitin-protein ligase DTX3L (DTX3L)

  • 货号：
    CSB-YP855054HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP855054HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP855054HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP855054HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP855054HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    B lymphoma and BAL associated protein; B-lymphoma- and BAL-associated protein; BBAP; Deltex 3 like; DTX 3L; DTX3L; DTX3L_HUMAN; E3 ubiquitin-protein ligase DTX3L; Protein deltex 3 like; Protein deltex 3 like protein; Protein deltex-3-like; Rhysin 2; Rhysin-2; Rhysin2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-740
  • 氨基酸序列
    ASHLRPPSP LLVRVYKSGP RVRRKLESYF QSSKSSGGGE CTVSTQEHEA PGTFRVEFSE RAAKERVLKK GEHQILVDEK PVPIFLVPTE NSIKKNTRPQ ISSLTQSQAE TPSGDMHQHE GHIPNAVDSC LQKIFLTVTA DLNCNLFSKE QRAYITTLCP SIRKMEGHDG IEKVCGDFQD IERIHQFLSE QFLESEQKQQ FSPSMTERKP LSQQERDSCI SPSEPETKAE QKSNYFEVPL PYFEYFKYIC PDKINSIEKR FGVNIEIQES SPNMVCLDFT SSRSGDLEAA RESFASEFQK NTEPLKQECV SLADSKQANK FKQELNHQFT KLLIKEKGGE LTLLGTQDDI SAAKQKISEA FVKIPVKLFA ANYMMNVIEV DSAHYKLLET ELLQEISEIE KRYDICSKVS EKGQKTCILF ESKDRQVDLS VHAYASFIDA FQHASCQLMR EVLLLKSLGK ERKHLHQTKF ADDFRKRHPN VHFVLNQESM TLTGLPNHLA KAKQYVLKGG GMSSLAGKKL KEGHETPMDI DSDDSKAASP PLKGSVSSEA SELDKKEKGI CVICMDTISN KKVLPKCKHE FCAPCINKAM SYKPICPTCQ TSYGIQKGNQ PEGSMVFTVS RDSLPGYESF GTIVITYSMK AGIQTEEHPN PGKRYPGIQR TAYLPDNKEG RKVLKLLYRA FDQKLIFTVG YSRVLGVSDV ITWNDIHHKT SRFGGPEMYG YPDPSYLKRV KEELKAKGIE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses. Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4. In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication. Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM. In addition, required for the recruitment of HGS and STAM to early endosomes. In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteosomal-mediated degradation.
  • 基因功能参考文献：
    1. Immunohistochemical analysis demonstrated that DTX3L was highly expressed in the glioma tissues and its level was correlated with the grade of malignancy. Multivariate analysis revealed the association between high expression of DTX3L and the poor prognosis of glioma patients. In addition, knockdown of DTX3L by siRNA transfection increased glioma cell apoptosis. PMID: 28627634
    2. we also found that DTX3L expression was regulated by focal adhesion kinase. Taken together, the results of this study show that DTX3L plays an important role in the proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells and as such may play a key role in the development of multiple myeloma. PMID: 28653881
    3. A novel role for the really interesting new gene-domain E3 ubiquitin ligase deltex-3-like (DTX3L) in regulating CXCR4 sorting from endosomes to lysosomes. PMID: 24790097
    4. we report the high-resolution crystal structure of this previously uncharacterized C-terminal domain of human DTX3L, which we term the Deltex C-terminal domain. PMID: 22411408
    5. Overexpression of DTX3L, PIK3R4, ATP2C1, and SLC25A36, all located at 3q21.1-23 are associated with cervical cancer. PMID: 18618715

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Deltex family
  • 数据库链接：

    HGNC: 30323

    OMIM: 613143

    KEGG: hsa:151636

    STRING: 9606.ENSP00000296161

    UniGene: Hs.518201