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  4. Recombinant Human E3 ubiquitin-protein ligase RING2 (RNF2)

Recombinant Human E3 ubiquitin-protein ligase RING2 (RNF2)

  • 中文名称：
    人RNF2重组蛋白
  • 货号：
    CSB-YP859101HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RNF2重组蛋白
  • 货号：
    CSB-EP859101HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RNF2重组蛋白
  • 货号：
    CSB-EP859101HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RNF2重组蛋白
  • 货号：
    CSB-BP859101HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RNF2重组蛋白
  • 货号：
    CSB-MP859101HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RNF2
  • Uniprot No.：
  • 别名：
    BAP 1; BAP1; DING; DinG protein; E3 ubiquitin protein ligase RING 2; E3 ubiquitin protein ligase RING2; E3 ubiquitin-protein ligase RING2; HIP2 interacting protein 3; HIP2-interacting protein 3; HIPI 3; HIPI3; Huntingtin interacting protein 2 interacting protein 3; Huntingtin-interacting protein 2-interacting protein 3; OTTHUMP00000060668; Polycomb M33 interacting protein Ring 1B; Polycomb M33 interacting protein Ring1B; Protein DinG; RING 1B; RING 2; RING finger protein 1B; RING finger protein 2; RING finger protein BAP 1; RING finger protein BAP-1; RING finger protein BAP1; RING1b; RING2_HUMAN; RNF 2; Rnf2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-336
  • 氨基酸序列
    SQAVQTNGT QPLSKTWELS LYELQRTPQE AITDGLEIVV SPRSLHSELM CPICLDMLKN TMTTKECLHR FCADCIITAL RSGNKECPTC RKKLVSKRSL RPDPNFDALI SKIYPSRDEY EAHQERVLAR INKHNNQQAL SHSIEEGLKI QAMNRLQRGK KQQIENGSGA EDNGDSSHCS NASTHSNQEA GPSNKRTKTS DDSGLELDNN NAAMAIDPVM DGASEIELVF RPHPTLMEKD DSAQTRYIKT SGNATVDHLS KYLAVRLALE ELRSKGESNQ MNLDTASEKQ YTIYIATASG QFTVLNGSFS LELVSEKYWK VNKPMELYYA PTKEHK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes. Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation.
  • 基因功能参考文献：
    1. these data indicate that RING1B and EZH2 repress the innate inflammatory cutaneous squamous cell carcinoma function and impair tumor immunosurveillance PMID: 29394319
    2. Observations demonstrated that Plk1 directly interacts with RNF2 and degrades RNF2 via the ubiquitindependent degradation pathway. RNF2 may be used as a new target for mitotic regulation and tumorigenesis. PMID: 29565459
    3. These data suggest that RNF2 is an important upstream negative regulator of SIK1 and that restoration of SIK1 levels induced by loss of RNF2 inhibited HCC cell growth and promoted apoptosis, which may represent a promising therapeutic strategy for HCC treatment. PMID: 27911266
    4. Study found that prostate cancer (PCa) tissues have high RNF2 expression which is positively correlated with tumor grade indicating that RNF2 may have oncogenic function in PCa. PMID: 28029659
    5. Our findings identify RING1B as a trait of the cell-of-origin in Ewing sarcoma PMID: 27317769
    6. Study detected RNF2 expression to be overexpressed in esophageal carcinoma cell lines and was associated with the poor prognosis of esophageal carcinoma patients. Data also reflect its important role in the growth of esophageal carcinoma cells by shRNA silencing of RNF2 expression. PMID: 26936624
    7. overexpression of RNF2, as examined by immunohistochemical analysis, might serve as a novel prognostic biomarker and potential therapeutic target for urothelial carcinoma of the bladder patients. PMID: 26869491
    8. Our findings support the notion that epigenetic regulators, such as RNF2, directly and functionally control powerful gene networks that are vital in multiple cancer processes. PMID: 26450788
    9. O-GlcNAc modifies and regulates an essential epigenetic tool, RING1B, which may contribute to human embryonic stem cells pluripotency maintenance and differentiation. PMID: 26100231
    10. The enzymatic activity of BMI1 and RING1B were evaluated and compared. PMID: 26151332
    11. Bmi1 and possibly RNF2 may be attractive biomarkers of gemcitabine resistance in the context of RRM1 expression. PMID: 24614341
    12. combinatorial silencing along with Ezh2 depleted epigenetically modified H2- and H3-histones and inhibited pancreatic cancer cell growth in vitro and in vivo (xenograft model) PMID: 25431952
    13. BAP1 deficiency causes increased expression of FoxK2 target genes in a Ring1B-Bmi1-dependent manner PMID: 25451922
    14. s describe a critical role for the polycomb group protein Ring1b in nonhomologous end-joining (NHEJ)-mediated end-to-end chromosome fusions. PMID: 24813883
    15. Ring1B and the SNAG-associated chromatin modifier EZH2 formed distinct protein complexes with Snail and that EZH2 was required for Snail-Ring1A/B recruitment to the target promoter. PMID: 24903147
    16. The acidic patch functions within the nucleosome as nucleosomes containing a mutated acidic patch exhibit defective H2A/H2AXub by RNF168 and RING1B/BMI1 in vitro PMID: 24603765
    17. Data indicate that RING finger protein 2 (RNF2) knockdown can increase the sensitivity of U87 cells to X ray radiation. PMID: 24796740
    18. Expressed the isolated N-terminal region of Ring1B, N-Ring1B, comprising the first 221 residues of the 334-residue-long Ring1B and found that the N-Ring1B is a well-folded, monomeric fragment, with native-like structure which unfolds irreversibly. PMID: 24284202
    19. BMI-1 and RING1B expression was enhanced with the development of embryos in early pregnancy. PMID: 23727134
    20. knockdown of RNF2 significantly inhibits both cell proliferation and colony formation and induces apoptosis in cancer cells PMID: 23318437
    21. Ring2 may effect the DNA repair through other pathways but not through the expressions of NER protein. PMID: 23712474
    22. Results indicate that RNF2 is an E3 ligase for p53 degradation in selective cells, implicating RNF2 as a therapeutic target to restore tumor suppression through p53 in certain tumor cells. PMID: 23319651
    23. the reduction of LPS-mediated IL-8 promoter activation was not related to de novo X-DING-CD4 protein synthesis, but depended on function of the exogenous X-DING-CD4 protein. PMID: 22031506
    24. These data provide evidence that the X-DING-CD4 gene contributes to early cellular protection from HIV infection in some individuals and this protection depends solely on the unique genetic regulation of the host. PMID: 22042911
    25. The organism reacts to HIV-infection by an overexpression of DING proteins. PMID: 22427948
    26. Crystallography of a complex of the Bmi1/Ring1b RING-RING heterodimer & UbcH5c shows that UbcH5c interacts with Ring1b only. Bmi1/Ring1b interacts with nucleosomal DNA & an acidic patch on histone H4 to achieve specific monoubiquitination of H2A. PMID: 21772249
    27. identification of genes regulated by ring finger protein 2 PMID: 21347701
    28. Polycomb Group protein targeting to different chromatin locations relies, in part, on binding partners of the C-terminal domain of RING1B that are diverse in sequence and structure. PMID: 20696397
    29. Ring1B plays an important role in the induction of T helper (Th)2 cell-driven allergic airway inflammation through the control of Bim-dependent apoptosis of effector T helper (Th)2 cells in vivo. PMID: 20237291
    30. polycomb protein Ring1B regulation by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome PMID: 20351251
    31. The human PcG protein dinG interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription. PMID: 11865070
    32. The E3 ubiquitin ligase RNF2 might have a dual function: facilitating the ubiquitination of its target substrates and recruiting the substrates to the proteasome. PMID: 15773819
    33. Bmi-1-Ring1B complex stabilizes the interaction of the ubiquitin ligase complex. PMID: 16714294
    34. Ring1B and Bmi1 are degraded by an exogenous E3, independent of their RING domain. The RING domains of both proteins mediate their association and subsequent stabilization. PMID: 17157253
    35. caspases-3 and caspase-9 play novel roles in transcription by regulating polycomb protein function through direct cleaving of Ring1B. PMID: 17379327
    36. With prohibitin, regulates the activity of E2F1 transcription factor via dual pathways. PMID: 17873902
    37. Results show that a phosphorylated form of Mel-18 targets the Ring1B histone H2A ubiquitin ligase to chromatin. PMID: 17936708
    38. Data show that the presence of flexible regions could allow C-terminal region of RING1B to bind a variety of different factors, ultimately recruiting RING1B and its associated PcG proteins to different genomic loci. PMID: 18616292
    39. a novel mechanism by which RNF2 and PHB2 modulate the CP2-mediated transcriptional pathway. PMID: 18629613
    40. Altered mRNA expression is associated with therapy failure and death in patients with multiple types of cancer. PMID: 15931389

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Chromosome.
  • 数据库链接：

    HGNC: 10061

    OMIM: 608985

    KEGG: hsa:6045

    STRING: 9606.ENSP00000356480

    UniGene: Hs.591490