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  4. Recombinant Human E3 ubiquitin-protein ligase TRIM50 (TRIM50)

Recombinant Human E3 ubiquitin-protein ligase TRIM50 (TRIM50)

  • 中文名称：
    人TRIM50重组蛋白
  • 货号：
    CSB-YP768234HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TRIM50重组蛋白
  • 货号：
    CSB-EP768234HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TRIM50重组蛋白
  • 货号：
    CSB-EP768234HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TRIM50重组蛋白
  • 货号：
    CSB-BP768234HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TRIM50重组蛋白
  • 货号：
    CSB-MP768234HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TRIM50
  • Uniprot No.：
  • 别名：
    E3 ubiquitin ligase ; E3 ubiquitin protein ligase TRIM50; E3 ubiquitin-protein ligase TRIM50; TRI50_HUMAN; TRIM50; TRIM50A ; Tripartite motif containing 50; Tripartite motif containing protein 50; Tripartite motif protein 50; tripartite motif protein 50A ; tripartite motif-containing 50A ; Tripartite motif-containing protein 50
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-487
  • 氨基酸序列
    MAWQVSLLEL EDWLQCPICL EVFKEPLMLQ CGHSYCKGCL VSLSCHLDAE LRCPVCRQAV DGSSSLPNVS LARVIEALRL PGDPEPKVCV HHRNPLSLFC EKDQELICGL CGLLGSHQHH PVTPVSTVYS RMKEELAALI SELKQEQKKV DELIAKLVNN RTRIVNESDV FSWVIRREFQ ELHHLVDEEK ARCLEGIGGH TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHKF IRKFHSMASR AEMPQARPLE GAFSPISFKP GLHQADIKLT VWKRLFRKVL PAPEPLKLDP ATAHPLLELS KGNTVVQCGL LAQRRASQPE RFDYSTCVLA SRGFSCGRHY WEVVVGSKSD WRLGVIKGTA SRKGKLNRSP EHGVWLIGLK EGRVYEAFAC PRVPLPVAGH PHRIGLYLHY EQGELTFFDA DRPDDLRPLY TFQADFQGKL YPILDTCWHE RGSNSLPMVL PPPSGPGPLS PEQPTKL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction.
  • 基因功能参考文献：
    1. TRIM50, via the RING domain, ubiquitinates Beclin 1 in a K63-dependent manner enhancing its binding with ULK1 and autophagy activity. PMID: 29604308
    2. TRIM50 K372 residue competes for post-translational protein modifications such as acetylation and ubiquitination. PMID: 24308962
    3. When the proteasome activity is impaired, TRIM50 fails to drive its substrates to the proteasome-mediated degradation. PMID: 22792322
    4. Encodes an E3 ubiquitin ligase; putatively involved in phenotype determination of Williams-Beuren syndrome. PMID: 18398435
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRIM/RBCC family
  • 数据库链接：

    HGNC: 19017

    OMIM: 612548

    KEGG: hsa:135892

    STRING: 9606.ENSP00000327994

    UniGene: Hs.647053