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  4. Recombinant Human ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1), partial

Recombinant Human ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1), partial

  • 货号：
    CSB-YP838793HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP838793HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP838793HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP838793HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP838793HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    EDEM1; EDEM; KIAA0212ER degradation-enhancing alpha-mannosidase-like protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.
  • 基因功能参考文献：
    1. EDEM1 colocalizes with the selective autophagy cargo receptors p62/SQSTM1, neighbor of BRCA1 gene 1 (NBR1) and autophagy-linked FYVE (Alfy) protein, and becomes engulfed by autophagic isolation membranes. PMID: 24664425
    2. Endoplasmic reticulum degradation-enhancing alpha-mannosidase-like protein 1 targets misfolded HLA-B27 dimers for endoplasmic reticulum-associated degradation. PMID: 25132672
    3. miR-581 promotes hepatitis B virus surface antigen expression by targeting Dicer and EDEM1 in HepG2 cells. PMID: 24913918
    4. EDEM1 associates with lifespan in animal models, although not humans. PMID: 24037343
    5. analysis of a shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins PMID: 23233672
    6. Tyrosinase degradation is prevented when EDEM1 lacks the intrinsically disordered region PMID: 22905195
    7. we suggest that a nascent glycoprotein can normally dissociate from EDEM1 and be rescued from endoplasmic reticulum associated degradation by reentering calnexin-refolding cycles PMID: 21917589
    8. signal sequence cleavage functionally regulated the association of EDEM1-soluble and membrane-integrated isoforms with distinct ERAD machinery and substrates. PMID: 21632540
    9. EDEM1 protein recognition may be determined by the structure of the endoplasmic reticulum-associated protein degradation substrate in a study of mutant ricin A-chain transport to the cytosol. PMID: 21388347
    10. mannose trimming enables delivery of a substrate glycoprotein from EDEM1 to late endoplasmic reticulum degredation steps through association with XTP3-B PMID: 21062743
    11. The EDEM1 activity trims mannose from the C branch of N-glycans in vivo. PMID: 20065073
    12. EDEM1 promotes correct folding and enhanced degradation of rod opsin. PMID: 19934218
    13. EDEM was shown to extract misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle [EDEM] PMID: 12610306
    14. EDEM1 is a soluble protein of the ER lumen in HEK293 cells PMID: 15579471
    15. These results indicate that EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation so that unstable misfolded proteins can be accommodated by the dislocon for ERAD. PMID: 16629899
    16. the endoplasmic reticulum quality control vesicular transport pathway using EDEM1 does not involve the COPII exit sites PMID: 17360537
    17. Cells with defective EDEM1 turnover contain unphysiologically high levels of EDEM1, show enhanced ER associated degradation activity and are characterized by impaired capacity to efficiently complete maturation of model glycopolypeptides. PMID: 18452703
    18. endogenous EDEM1 in cells not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy PMID: 19266160
    19. EDEM1 specifically binds nonnative proteins in a glycan-independent manner. PMID: 19524542

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Glycosyl hydrolase 47 family
  • 数据库链接：

    HGNC: 18967

    OMIM: 607673

    KEGG: hsa:9695

    STRING: 9606.ENSP00000256497

    UniGene: Hs.224616