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  4. Recombinant Human ETS homologous factor (EHF)

Recombinant Human ETS homologous factor (EHF)

  • 货号：
    CSB-YP007494HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007494HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007494HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007494HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007494HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EHF
  • Uniprot No.：
  • 别名：
    EHF; EHF_HUMAN; Epithelium-specific Ets transcription factor 3; ESE-3; ESE3; ESE3 transcription factor; ESE3B; ESEJ; Ets domain transcription factor; ETS domain-containing transcription factor; ETS homologous factor; hEHF
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-300
  • 氨基酸序列
    MILEGGGVMN LNPGNNLLHQ PPAWTDSYST CNVSSGFFGG QWHEIHPQYW TKYQVWEWLQ HLLDTNQLDA NCIPFQEFDI NGEHLCSMSL QEFTRAAGTA GQLLYSNLQH LKWNGQCSSD LFQSTHNVIV KTEQTEPSIM NTWKDENYLY DTNYGSTVDL LDSKTFCRAQ ISMTTTSHLP VAESPDMKKE QDPPAKCHTK KHNPRGTHLW EFIRDILLNP DKNPGLIKWE DRSEGVFRFL KSEAVAQLWG KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
  • 基因功能参考文献：
    1. A negative correlation between EHF protein and miR206 expression. PMID: 28714026
    2. Human prostate tumors with IL-6 elevation and loss of ESE3/EHF were associated with STAT3 activation and displayed upregulation of genes related to cell adhesion, cancer stem-like and metastatic spread. PMID: 27732936
    3. EHF is a novel functional oncogene in thyroid cancer by transcriptionally regulating HER2 and HER3, and may represent a potential therapeutic target for this cancer PMID: 27517321
    4. The role of EHF in modifying Cystic Fibrosis lung disease severity. PMID: 28549169
    5. is a novel functional oncogene in gastric cancer by regulating the human epidermal growth factor receptor (HER) family of receptor tyrosine kinases. PMID: 27787520
    6. tight control by ESE3/EHF over the Lin28/let-7 axis is a critical barrier to malignant transformation. PMID: 27197175
    7. ESE3 as a negative regulator of pancreatic ductal adenocarcinomas (PDAC) progression and metastasis by enforcing E-cadherin upregulation. PMID: 27923832
    8. EHF activates expression of the SAM pointed domain-containing ETS transcription factor, which contributes to goblet cell hyperplasia. PMID: 28461336
    9. Results found that EHF was elevated in ovarian cancer tissues and correlated with short survival. Its overexpression in ovarian cancer cells promoted cell growth and metastasis, and inhibited cell apoptosis. PMID: 26258986
    10. This study suggests that ESE3 plays an important role in the carcinogenesis of Esophageal Squamous Cell Carcinoma through changes in subcellular localization and may act as a tumor suppressor gene in Esophageal Squamous Cell Carcinoma. PMID: 25950810
    11. EHF modifies the CF phenotype by altering capabilities of the epithelial cell to correctly process the folding and trafficking of mutant p.Phe508del-CFTR. PMID: 24105369
    12. Results suggest PPAR-gamma to be involved in the regulation of ESE-3b expression during monocyte-derived dendritic cells (moDCs) development and that ESE-3 expression is not correlated with the immunogenicity of dendritic cells (DCs). PMID: 24219556
    13. The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model. PMID: 23439650
    14. ESE-3b is the functionally most important ESE-3 isoform in dendritic cells PMID: 23185370
    15. Low ESE3/EHF expression was also associated with increased biochemical recurrence of prostate cancer and reduced overall survival after prostatectomy PMID: 22505649
    16. EHF mRNA levels differentiate ovarian carcinoma from malignant mesothelioma. EHF may be a novel prognostic marker in ovarian carcinoma. PMID: 21855111
    17. These results indicate that EHF-mediated RUVBL1 expression allows colon tumour cells to avoid p53-mediated apoptosis. PMID: 21617703
    18. Overexpression of ESE-3 protein in human bronchial smooth muscle cells inhibits MMP-1 promoter activity, suggesting that ESE-3 may function as a transcriptional repressor. PMID: 12444029
    19. In addition, we show the possible involvement of co-factors CBP and p300 in ESE-3-mediated DR-5 up-regulation. PMID: 17027647
    20. Aberrant expression of EHF and the subsequent disruption of p27-mediated senescence and telomerase activity is likely to contribute significantly to tumor progression. EHF might be a promising target for future cancer therapeutics. PMID: 17172423
    21. ESE-3 plays a role in the induction of cellular senescence as a downstream molecule of stress-induced mitogen-activated protein kinase p38. PMID: 17627613
    22. Implicated as a candidate tumor suppressor in prostate cancer; decreased expression may result in loss of important regulatory mechanisms in prostate epithelial cells. PMID: 18037958
    23. ESE-1 and ESE-3 play an important role in airway inflammation. PMID: 18475289

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ETS family
  • 组织特异性：
    Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen,
  • 数据库链接：

    HGNC: 3246

    OMIM: 605439

    KEGG: hsa:26298

    STRING: 9606.ENSP00000435835

    UniGene: Hs.653859