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  4. Recombinant Human Equilibrative nucleoside transporter 4 (SLC29A4), partial

Recombinant Human Equilibrative nucleoside transporter 4 (SLC29A4), partial

  • 中文名称：
    人SLC29A4重组蛋白
  • 货号：
    CSB-YP745761HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SLC29A4重组蛋白
  • 货号：
    CSB-EP745761HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SLC29A4重组蛋白
  • 货号：
    CSB-EP745761HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SLC29A4重组蛋白
  • 货号：
    CSB-BP745761HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SLC29A4重组蛋白
  • 货号：
    CSB-MP745761HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SLC29A4
  • Uniprot No.：
  • 别名：
    SLC29A4; ENT4; PMAT; PSEC0113; Equilibrative nucleoside transporter 4; hENT4; Plasma membrane monoamine transporter; Solute carrier family 29 member 4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as a polyspecific organic cation transporter, efficiently transporting many organic cations such as monoamine neurotransmitters 1-methyl-4-phenylpyridinium and biogenic amines including serotonin, dopamine, norepinephrine and epinephrine. May play a role in regulating central nervous system homeostasis of monoamine neurotransmitters. May be involved in luminal transport of organic cations in the kidney and seems to use luminal proton gradient to drive organic cation reabsorption. Does not seem to transport nucleoside and nucleoside analogs such as uridine, cytidine, thymidine, adenosine, inosine, guanosine, and azidothymidine. In adenosine is efficiently transported but in a fashion highly sensitive to extracellular pH, with maximal activity in the pH range 5.5 to 6.5. Glu-206 is essential for the cation selectivity and may function as the charge sensor for cationic substrates. Transport is chloride and sodium-independent but appears to be sensitive to changes in membrane potential. Weakly inhibited by the classical inhibitors of equilibrative nucleoside transport, dipyridamole, dilazep, and nitrobenzylthioinosine. May play a role in the regulation of extracellular adenosine concentrations in cardiac tissues, in particular during ischemia.
  • 基因功能参考文献：
    1. Cultured astroctye line 1321N1 and primary human astrocytes transport monoamines predominantly through PMAT. PMID: 24471494
    2. Transport kinetic analysis revealed that I89M mutant in PMAT protein had a 2.7-fold reduction in maximal transport velocity with no significant change in apparent binding affinity. PMID: 22562044
    3. Data show that ENT1, ENT2, ENT4 and CNT3 protein was detected on ovarian carcinoma cells in all effusions, with expression observed in 1-95% of tumor cells. PMID: 21822668
    4. The selectivity and kinetics of monoamine neurotransmitter transport by PMAT and OCT3 was determined. PMAT is expressed at much higher levels than hOCT3 in brain, whereas hOCT3 is selectively and highly expressed in adrenal gland and skeletal muscle. PMID: 20858707
    5. Observational study of gene-disease association. (HuGE Navigator) PMID: 20392501
    6. may be a novel low affinity transporter for biogenic amines, might supplement the high affinity transporters in the brain PMID: 15448143
    7. PMAT can function as a polyspecific organic cation transporter, which may play a role in organic cation transport in vivo. PMID: 16099839
    8. ENT4, in addition to playing roles in cardiac serotonin transport, contributes to the regulation of extracellular adenosine concentrations, in particular under the acidotic conditions associated with ischemia. PMID: 16873718
    9. our data suggest that PMAT is expressed on the apical membranes of renal epithelial cells and may use luminal proton gradient to drive organic cation reabsorption in the kidney. PMID: 17018840
    10. Amino acid substitution at several sites results in loss of activity ane conformation. PMID: 17121826
    11. PMAT, EMT, and OCT2 transporters are expressed in the endometrial stroma and can potentially regulate reuptake of monoamines in general and histamine in particular. PMID: 17393420
    12. identification of ENT4 as a highly expressed transcript in desmoplastic small round cell tumor (DSRCT) may represent an attractive pathway for targeting chemotherapeutic drugs into DSRCT PMID: 18523561
    13. The data suggest that PMAT is specifically expressed in podocytes and may play an important role in puromycin aminonucleoside-induced kidney injury. PMID: 19357181

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SLC29A/ENT transporter (TC 2.A.57) family
  • 组织特异性：
    Expressed abundantly in the heart, in both cardiomyocytes and vascular endothelial cells (at protein level). Highly expressed in brain, kidney and skeletal muscle. In the brain expressed in cerebellum, cerebral cortex, medulla, occipital pole, frontal and
  • 数据库链接：

    HGNC: 23097

    OMIM: 609149

    KEGG: hsa:222962

    STRING: 9606.ENSP00000297195

    UniGene: Hs.4302