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  4. Recombinant Human Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1)

Recombinant Human Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1)

  • 货号：
    CSB-EP009966HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009966HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-MP009966HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Name:GSPT1 Synonyms:ERF3A
  • Uniprot No.：
  • 别名：
    GSPT1; ERF3AEukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-499
  • 氨基酸序列
    MELSEPIVEN GETEMSPEES WEHKEEISEA EPGGGSLGDG RPPEESAHEM MEEEEEIPKP KSVVAPPGAP KKEHVNVVFI GHVDAGKSTI GGQIMYLTGM VDKRTLEKYE REAKEKNRET WYLSWALDTN QEERDKGKTV EVGRAYFETE KKHFTILDAP GHKSFVPNMI GGASQADLAV LVISARKGEF ETGFEKGGQT REHAMLAKTA GVKHLIVLIN KMDDPTVNWS NERYEECKEK LVPFLKKVGF NPKKDIHFMP CSGLTGANLK EQSDFCPWYI GLPFIPYLDN LPNFNRSVDG PIRLPIVDKY KDMGTVVLGK LESGSICKGQ QLVMMPNKHN VEVLGILSDD VETDTVAPGE NLKIRLKGIE EEEILPGFIL CDPNNLCHSG RTFDAQIVII EHKSIICPGY NAVLHIHTCI EEVEITALIC LVDKKSGEKS KTRPRFVKQD QVCIARLRTA GTICLETFKD FPQMGRFTLR DEGKTIAIGK VLKLVPEKD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ETF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.
  • 基因功能参考文献：
    1. PABP enhances the productive binding of the eRF1-eRF3 complex to the ribosome, via interactions with the N-terminal domain of eRF3a which itself has an active role in translation termination. PMID: 27418677
    2. Data found that the N-terminal glycine repeat of eRF3a influences eRF3a-PABP interaction and that eRF3a 12-GGC allele has a decreased binding affinity for PABP. PMID: 26818177
    3. GAB2, GSPT1, TFDP2 and ZFPM1 are four new susceptibility loci for testicular germ cell tumor. PMID: 26503584
    4. The survivin and eRF3 complex may function in spindle formation and segregation of chromosomes and cytokinesis. PMID: 23377885
    5. Overexpression of ID1 in two different cell lines induced STMN3 and GSPT1 at the transcriptional level, while depletion of ID1 reduced their expression. PMID: 25028095
    6. The proteolytic cleavage of eRF3a and eRF3b into p-eRF3 leads to release an amino-terminal fragment containing nuclear export signal to allow the relocalization of eRF3 into the nucleus to interact with the p14ARF. PMID: 24569073
    7. ERF3 is targeted for caspase-mediated proteolytic cleavage and degradation during DNA damage-induced apoptosis. PMID: 23054082
    8. Our results show that the presence of the longer allele of eRF3a is correlated with threefold increased risk of breast cancer development PMID: 22101789
    9. A biological role for the overlapping ERF3 PAM2 motifs in the regulation of deadenylase accessibility to PABPC1 at the 3' end of poly(A). PMID: 23019593
    10. crystal structures of the MLLE domain from PABPC1 in complex with the two PAM2 regions of eRF3 PMID: 20418951
    11. GSPT1/eRF3 is proteolytically processed into an IAP-binding protein PMID: 12865429
    12. Human translation termination factor eRF3/GSPT1 is an interacting partner of RNase L. PMID: 15908960
    13. Polyglycine expansions in eukaryotic translation release factor 3 are associated with gastric cancer susceptibility PMID: 15987717
    14. eRF3a controls the formation of the termination complex by modulating eRF1 protein stability. PMID: 15987998
    15. Further, we describe a novel complex that contains the NMD factors SMG-1 and Upf1, and the translation termination release factors eRF1 and eRF3 (SURF). an association between SURF and the EJC is required for SMG-1-mediated Upf1 phosphorylation and NMD. PMID: 16452507
    16. GSTP1 inhibits the binding of MAP kinase kinase kinase 5 (MAP3K5) to the 14-3-3 protein, a MAP3K5 inhibitor, while a novel role of GSPT1 is revealed in the regulation of apoptosis signal-regulating kinase 1 (ASK1)-mediated apoptosis. PMID: 17700517
    17. eRF3a is degraded by the proteasome when not associated with eRF1; proteasomal degradation of eRF3a controls translation termination complex formation by adjusting the eRF3a level to that of eRF1. PMID: 18083835
    18. eRF3a/GSPT1 12-GGC allele increases the susceptibility for breast cancer development. PMID: 19424636
    19. GSPT1 GGC(12) allele was present in 2.2% of colorectal cancer patients but was absent in Crohn disease patients and in the control group PMID: 19963113

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  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, ERF3 subfamily
  • 数据库链接：

    HGNC: 4621

    OMIM: 139259

    KEGG: hsa:2935

    STRING: 9606.ENSP00000398131

    UniGene: Hs.528780