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  4. Recombinant Human Fascin (FSCN1)

Recombinant Human Fascin (FSCN1)

  • 货号：
    CSB-YP619084HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP619084HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP619084HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP619084HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP619084HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FSCN1
  • Uniprot No.：
  • 别名：
    55 kDa actin bundling protein; 55 kDa actin-bundling protein; Actin bundling protein; actin bundling protein; 55-KD; FAN 1; FAN1; Fascin 1; Fascin actin bundling protein 1; Fascin; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus); Fascin homolog 1; Fascin; sea urchin; homolog of; 1; Fascin1; FLJ38511; FSCN 1; FSCN1; FSCN1_HUMAN; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); Singed drosophila homolog like; Singed like (fascin homolog sea urchin) (Drosophila); Singed like (fascin homolog sea urchin); Singed like protein; Singed; drosophila; homolog of; Singed-like protein; SNL; Strongylocentrotus purpuratus
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-493
  • 氨基酸序列
    TANGTAEAV QIQFGLINCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV CLRSHLGRYL AADKDGNVTC EREVPGPDCR FLIVAHDDGR WSLQSEAHRR YFGGTEDRLS CFAQTVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT ADHRFLRHDG RLVARPEPAT GYTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR DTKKCAFRTH TGKYWTLTAT GGVQSTASSK NASCYFDIEW RDRRITLRAS NGKFVTSKKN GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV TGTLDANRSS YDVFQLEFND GAYNIKDSTG KYWTVGSDSA VTSSGDTPVD FFFEFCDYNK VAIKVGGRYL KGDHAGVLKA SAETVDPASL WEY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.
  • 基因功能参考文献：
    1. fascin1 constitutively interacts with IkappaB kinase (IKK) in the RIG-I signaling pathway. In summary, we have identified fascin1 as a suppressor of the RIG-I signaling pathway associating with IkappaB kinase in DLD-1 colon cancer cells to suppress immune responses to viral infection. PMID: 29496994
    2. fascin protein accumulation, caused by reduced proteasomal activity, contributes to the acquisition of cancer stemness in chronic inflammation-related colon carcinogenesis. PMID: 30056535
    3. Fascin1 is related with clinicopathologic parameters of gastric cancer and overexpressed both in gastric cell lines and gastric tumor tissue PMID: 30060981
    4. the expression of Fascin_1 protein differed between cancer tissue and paracarcinoma tissues in NSCLC patients and it was also correlated with poor prognosis. PMID: 29970665
    5. FSCN1 physiologically interacted with and increased the levels of snail1 to promote ovarian cancer cell epithelial-mesenchymal transition . PMID: 30231243
    6. fascin1 is an important mediator of TGF-beta1-induced invasion and migration of kidney carcinoma cells through ERK and JNK signal pathways. PMID: 29772238
    7. findings demonstrate that fascin is required for migration and invasion induced by LA in MDA-MB-231 breast cancer cells. PMID: 29052029
    8. PCAT-1 accelerated prostate cancer cell proliferation, migration, invasion and suppressed apoptosis by up-regulating FSCN1 mediated via miR-145-5p. PMID: 28922730
    9. Lentivirus-mediated fascin-1 knockdown significantly decreased the proliferation of non-small cell lung cancer cells. Furthermore, fascin-1 silencing partly inhibited cell invasion and migration. Inhibition of fascin-1 decreased the activity of the MAPK pathway. PMID: 29458026
    10. Study shows that Fascin 1 has a nuclear function in the regulation of the amino-acid transporter SLC3A2. PMID: 27819326
    11. SNAI2 overexpression significantly increased FSCN1 expression at both mRNA and protein level. FSCN1 overexpression reduced the expression of E-cadherin and Claudin 1, but increased the expression of Vimentin and N-cadherin in SCC9 and SCC-15 cells. It is inferred that FSCN1 is a downstream effector of SNAI2 in promoting EMT in HNSC cells. PMID: 28488774
    12. MYC-nick, fascin, and Cdc42 are frequently up-regulated in cells present at the invasive front of human colorectal tumors, suggesting a coordinated role for these proteins in tumor migration. PMID: 27566402
    13. The prognostic importance of TGF-beta, TGF-beta receptor, and fascin in childhood solid tumors PMID: 29065267
    14. The results reveal that phosphorylation at FSCN1 amino acids Y23, S38, S39 and S274, in combination, downregulates the extent of anchorage-independent growth, cell migration and filopodia formation in esophageal squamous cancer cells. PMID: 28251354
    15. Fascin and alpha-actinin intrinsically segregate to discrete bundled domains that are specifically recognized by other actin-binding proteins. PMID: 27666967
    16. This study showed that fascin expression intensity may be correlated with clinical outcomes of a newly diagnosed primary glioblastoma. PMID: 27406586
    17. These data demonstrated fascin as a critical regulator of breast cancer stem cell pool at least partially via activation of the Notch self-renewal signaling pathway. PMID: 27502039
    18. These results suggest that strong positive fascin-1 expression can be used as a diagnostic marker of TNBC. PMID: 27184764
    19. s demonstrated that one miRNA, miR-29a-5p, can bind to both the XIAP and FSCN1 3'UTRs and play an important role in that interactions. We showed that the 3'UTR of XIAP was able to antagonize miR-29a-5p, and resulted in the increased translation of XIAP and FSCN1. PMID: 28186968
    20. The expression of fascin-1 and lanminin-5 and the serum levels of fascin-1 and laminin-5 were related to the relapse of patients with non-small cell lung cancer. PMID: 28653739
    21. The results uncover a role for fascin that operates independently of filopodia assembly to promote efficient cell migration and invasion. PMID: 27554857
    22. FSCN1 is a direct target of miR-539.MiR-539 inhibits FSCN1 expression and suppresses hepatocellular carcinoma migration and invasion. PMID: 28393215
    23. we show for the first time that HTLV-1 usurps the host cell factor Fascin to foster virus release and cell-to-cell transmission. PMID: 27776189
    24. Results show that BMI1 and FSCN1 are highly expressed in colorectal neoplasm and correlate with poor prognosis. PMID: 27488323
    25. miR-200b serves a suppressive role in the mediation of NSCLC cell migration and invasion, partly at least, via targeting FSCN1. PMID: 27356635
    26. LRIG1 and Fascin-1 were differently expressed in cancer and normal lung tissue in patients with NSCLC, which could be a biomarker for mediastinal lymph node metastasis in NSCLC patients. PMID: 28230028
    27. The increase in fascin1 expression may be the key point of this impact of TGF-beta1. PMID: 27350089
    28. Fascin 1 plays an oncogenic role in non-small cell lung cancer PMID: 26886283
    29. fascin expression promotes cholangiocarcinoma RBE cell proliferation, migration, and invasion. PMID: 25882880
    30. TGF-beta1 could induce the expressions of Fascin1 mRNA and protein and thus improve the invasive and metastatic ability of human renal carcinoma cells. PMID: 26749573
    31. elevated L-plastin expression promotes elongation and reduces protrusion density in cells with relatively lower L-plastin than fascin levels. PMID: 26945069
    32. Study demonstrates that FSCN1 is associated with increased risk of recurrence in pituitary adenomas and invasion, partially through modifying the expression of Notch/DLL pathway. PMID: 26522130
    33. The present study revealed an antioncogenic role of miR145 in gastric carcinoma via inhibition of FSCN1, and suggested that miR145 may be used for the treatment of gastric carcinoma. PMID: 26647829
    34. miR-145 negatively regulates the protein expression of its target FSCN1 in the NSCLC cell line H129. PMID: 26238532
    35. MiR-24 functions as a tumor suppressor in nasopharyngeal carcinoma through targeting FSCN1 PMID: 26503504
    36. Strong fascin-1 expression was found in 14.3% of borderline epithelial tumors of the ovary and linked to features related to elevated risk of recurrence. PMID: 26362828
    37. FSCN1 plays an important role in non-small cell lung cancer progression and prognosis. PMID: 26211585
    38. Alterations in nuclear Fascin levels result in defects in nucleolar morphology in both Drosophila follicles and cultured mammalian cells, suggesting that nuclear Fascin plays an important role in nucleolar architecture. PMID: 25808493
    39. miR-145 regulates cell migration and invasion in gastric cancer primarily by directly targeting FSCN1. PMID: 26010149
    40. regulates translation through miR-155 and miR-125b, which target 3' UTR in TNF-alpha mRNA PMID: 25831081
    41. the anti-oncogenic activity of miR-133a may involve the inhibition of the target gene FSCN1. The present study suggested that miR-133a may be a potential therapeutic target in the treatment of gastric cancer. PMID: 25815687
    42. The up-regulation of FSCN1 is a prognostic factor in patients diagnosed with hepatocellular liver cancer. PMID: 26173501
    43. Upregulated fascin expression was detected in melanoma metastases, but was not correlated to patient outcome. PMID: 25535872
    44. miR-133b directly targeted FSCN1 which functioned as an oncogenic gene in gastric cancer cells. PMID: 25433493
    45. Fascin-1 and paxillin were expressed in 58% and 43% of infiltrating duct carcinoma cases. There was a significant correlation between fascin-1 and paxillin expression and tumor grade, clinical stage, lymph-node metastasis grade, and HER2 expression. PMID: 26349603
    46. The cortactin SH3 domain contributes to podosome assembly while fascin actin bundling is a master regulator of podosome disassembly in THP-1 macrophages and dendritic cells. PMID: 25601713
    47. In ovarian primary mucinous tumors, fascin and EMMPRIN may play an important role in tumor progression from benign tumor to carcinoma. PMID: 25155376
    48. miR-133a functions as a tumor suppressor and directly targets FSCN1 in pancreatic cancer. PMID: 25198665
    49. LMP1-mediated upregulation of Fascin depends on NF-kappaB and both NF-kappaB and Fascin contribute to invasive migration of LMP1-expressing lymphocytes. PMID: 25105941
    50. Fascin plays a role in stress fiber organization and focal adhesion disassembly. PMID: 24930964

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  • 亚细胞定位：
    Cytoplasm, cytosol. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell projection, filopodium. Cell projection, invadopodium. Cell projection, microvillus. Cell junction.
  • 蛋白家族：
    Fascin family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 11148

    OMIM: 602689

    KEGG: hsa:6624

    STRING: 9606.ENSP00000371798

    UniGene: Hs.118400