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  4. Recombinant Human Fermitin family homolog 3 (FERMT3)

Recombinant Human Fermitin family homolog 3 (FERMT3)

  • 货号：
    CSB-YP771441HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP771441HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP771441HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP771441HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP771441HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FERMT3
  • Uniprot No.：
  • 别名：
    Fermitin family homolog 3; Fermitin family member 3; FERMT3; Kind3; Kindlin 3; Kindlin-3; Kindlin3; MGC10966; MIG 2; MIG2 like protein; MIG2-like protein; MIG2B; Unc 112 related protein 2; Unc-112-related protein 2; UNC112C; URP2; URP2_HUMAN; URP2SF
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-667
  • 氨基酸序列
    MAGMKTASGD YIDSSWELRV FVGEEDPEAE SVTLRVTGES HIGGVLLKIV EQINRKQDWS DHAIWWEQKR QWLLQTHWTL DKYGILADAR LFFGPQHRPV ILRLPNRRAL RLRASFSQPL FQAVAAICRL LSIRHPEELS LLRAPEKKEK KKKEKEPEEE LYDLSKVVLA GGVAPALFRG MPAHFSDSAQ TEACYHMLSR PQPPPDPLLL QRLPRPSSLS DKTQLHSRWL DSSRCLMQQG IKAGDALWLR FKYYSFFDLD PKTDPVRLTQ LYEQARWDLL LEEIDCTEEE MMVFAALQYH INKLSQSGEV GEPAGTDPGL DDLDVALSNL EVKLEGSAPT DVLDSLTTIP ELKDHLRIFR IPRRPRKLTL KGYRQHWVVF KETTLSYYKS QDEAPGDPIQ QLNLKGCEVV PDVNVSGQKF CIKLLVPSPE GMSEIYLRCQ DEQQYARWMA GCRLASKGRT MADSSYTSEV QAILAFLSLQ RTGSGGPGNH PHGPDASAEG LNPYGLVAPR FQRKFKAKQL TPRILEAHQN VAQLSLAEAQ LRFIQAWQSL PDFGISYVMV RFKGSRKDEI LGIANNRLIR IDLAVGDVVK TWRFSNMRQW NVNWDIRQVA IEFDEHINVA FSCVSASCRI VHEYIGGYIF LSTRERARGE ELDEDLFLQL TGGHEAF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).; Isoform 2 may act as a repressor of NF-kappa-B and apoptosis.
  • 基因功能参考文献：
    1. FERMT3 regulates glioma cell activity through integrin-mediated Wnt/beta-catenin signaling. PMID: 28778805
    2. Expression of Kindlin2 is associated with tumor grade. PMID: 28677779
    3. this case report describes a female Thai patient who was diagnosed with leukocyte adhesion deficiency type III with a novel mutation in FERMT3, presenting with a humoral immune defect PMID: 26359933
    4. kindlin-3 plays an important role in maintaining a proper conformation of the resting alpha4beta1 to mediate both rolling and firm cell adhesion PMID: 26994136
    5. Kindlin-3 regulates c-Myc protein expression in the human chronic myeloid leukemia cell line K562. PMID: 26677948
    6. NMR studies demonstrated that the F1 loop of kindlin-3 is globally unfolded but stretches of residues assuming transient helical conformations could be detected in aqueous solution. PMID: 27101375
    7. Suppression of the Kindlin-2-integrin beta1-AKT regulatory axis is an alternative mechanism underlying the tumor suppressor function of miR-200b in esophageal squamous cell carcinoma. PMID: 26334393
    8. Kindlin-3/FERMT3 is upregulated in atherosclerotic, mainly in cells of monocytic origin and of M2 type. Simultaneous upregulation of ITGB2 suggests a synergistic effect on leukocyte adherence and transmigration into the vessel wall. PMID: 26188538
    9. The accumulation of HSPCs in the circulation of leukocyte adhesion deficiency type III patients, who lack Kindlin-3, underlines the conserved functions of Kindlin-3 in man and the importance of our findings for human disease. PMID: 26282877
    10. A new C>T point mutation was found in exon 13 in the FERMT3 gene in an infant diagnosed with LAD-III. KINDLIN-3 expression is required for platelet aggregation and leukocyte function, but also osteoclast-mediated bone resorption. PMID: 25854317
    11. Mig-2 significantly attenuates the antitumor action of cisplatin. PMID: 25152024
    12. The data uncover a novel and unexpected tumor suppressor role of Kindlin-3 which can influence integrins targeted therapies development. PMID: 25344860
    13. High Kindlin-2 expression promotes pancreatic ductal adenocarcinoma progression. PMID: 25724625
    14. These data identify a role of kindlin-3 phosphorylation in integrin b3 activation and provide a basis for functional differences between kindlin-3 and the two other kindlin paralogs. PMID: 25609252
    15. Kindlin 2 expression was significantly increased in luteinized granulosa cells from patients with polycystic ovary syndrome. PMID: 24336678
    16. kindlin-2 tyrosine phosphorylation and interaction with Src serve as a regulatable switch downstream of focal adhesion kinase in the integrin outside-in signaling circuit controlling cell migration and proliferation PMID: 25237194
    17. Direct activation of RhoA with recombinant bacterial cytotoxic necrotizing factor y reverted the abnormal phenotype and barrier function of kindlin-2-deficient keratinocytes and skin equivalents. PMID: 24615351
    18. ADAP interacts with talin and kindlin-3 to promote platelet Integrin alphaIIbbeta3 activation and stable fibrinogen binding. PMID: 24523237
    19. Kindlin-3 influences breast cancer progression by influencing the crosstalk between beta1 integrins and Twist to increase VEGF production, which enhances breast cancer cell invasion and tumor angiogenesis and metastasis PMID: 24469992
    20. While Kindlin-2 was highly expressed in control tissues, a drastic low expression of Kindlin-2 was found in the tumor tissues of serous epithelial ovarian cancer, especially in high-grade serous epithelial ovarian cancer. PMID: 24583125
    21. kindlin-3-mediated high-affinity LFA-1 controls both the early transient integrin-dependent adhesions in addition to the final stable adhesions made under flow conditions PMID: 24010654
    22. the beta-2-integrin-kindlin-3 interaction is particularly important in adhesion strengthening under shear flow, and for T-cell homing to lymph nodes, but dispensable for T cell activation which occurs in a shear-free environment PMID: 23823319
    23. kindlin-3 is required for the integrin alphaMbeta2-Syk-Vav1 signaling axis that regulates Rac1 and Cdc42 activities. These findings reinforce a role for kindlin-3 in integrin outside-in signaling. PMID: 23437269
    24. the correct functioning of the kindlin 3 PH domain is central to the role that kindlin 3 performs in guiding lymphocyte adhesion and motility behavior, which in turn is required for a successful immune response. PMID: 23595985
    25. Agonist stimulation, talin-1, and kindlin-3 are crucial for alpha(IIb)beta(3) activation in a human megakaryoblastic cell line, CMK. PMID: 23022222
    26. cleavage of Kindlin-3 by calpain controls the dynamics of integrin-Kindlin-3 interaction and as a result, integrin-dependent adhesion and migration of hematopoietic cells. PMID: 23012377
    27. Multivalent LFA-1/ICAM-1 bonds serve as mechanosensors that direct PMN cytoskeletal activity by transmission of tensile force to a supramolecular complex that triggers Ca2+ influx at sites of adhesive contact. PMID: 23144497
    28. kindlin-3 lowers the threshold for NK cell activation. Loss of kindlin-3 has a pronounced effect on NK cell-mediated cytotoxicity triggered by single activating receptors. PMID: 22983444
    29. Kindlin-3 deficiency impairs integrin function, including activation of beta(1) integrin. Abnormalities in glycoprotein Ib-IX function in Kindlin-3-deficient platelets are secondary to integrin defects PMID: 22564402
    30. integrin alphaLbeta2 engagement by its ligand ICAM-1 promotes the association of kindlin-3 with RACK1 PMID: 22334666
    31. LAD-III syndrome is caused by mutations in FERMT3, encoding the kindlin-3 protein in all blood cells that is involved in the regulation of beta integrin conformation. (Review) PMID: 22134107
    32. TIIICBP and kindlin-3 could be the same protein and propose a key role for kindlin-3 in platelet activation by type III collagen. PMID: 21871525
    33. A new mutation in codon 476 of exon 12, CAG-->TAG was found in 2 siblings with Leukocyte adhesion deficiency-I variant syndrome. PMID: 22139635
    34. We investigated the role of talin-1, kindlin-3, and alpha-actinin-1 in the upregulation of alpha(4)beta(1) integrin affinity and consequent inflammatory leukocyte adhesive events PMID: 21911599
    35. investigation into roles of kindlin-3 in activated T-cells: facilitates multimerization of LFA-1/ICAM-1; increases stability of focal LFA-1 contacts (presumably at immunological synapses)and anchors to cytoskeleton; facilitates T-cell spreading PMID: 21536861
    36. in two brothers with LAD-III syndrome homozygous mutation 1717C>T causes integrin-dependent platelet dysfunction in siblings with leukocyte adhesion deficiency-III PMID: 20216991
    37. LAD-III patient mutations have highlighted functionally important regions of kindlin-3 that alter leukocyte integrin-dependent function in 2 distinct ways. PMID: 20357244
    38. integrin co-activator Kindlin-3 is expressed and functional in a non-hematopoietic cell, the endothelial cell PMID: 20378539
    39. cellular functions and possible clinical relevance of kindlin-3 [REVIEW] PMID: 19854292
    40. Studies indicate that LAD I is due to the mutations in beta2-integrin essential for firm adhesion. PMID: 19647987
    41. expressed preferentially in B cells; MIG2B is in a highly conserved and defined gene family containing two plasma-membrane-binding ezrin/radixin/moesin domains and a pleckstrin homology domain PMID: 12886250
    42. our data show for the first time that URP2SF may act as a transcriptional repressor in NF-kappaB signaling pathway and regulate cell apoptotic pathway. PMID: 18280249
    43. the LAD-III phenotype could manifest a combined defect in both upstream and downstream integrin regulatory effectors CalDAG-GEFI and Kindlin-3 PMID: 18779414
    44. Mutations in FERMT3 is associated with Leukocyte adhesion deficiency syndrome. PMID: 19064721
    45. Kindlin-3 is essential to activate the beta1, beta2 and beta3 integrin classes, and loss of Kindlin-3 function is sufficient to cause a LAD-III-like phenotype in mice PMID: 19234461
    46. identify mutations in the KINDLIN3 (official symbol FERMT3) gene specifying the KINDLIN-3 protein as the cause of leukocyte adhesion deficiency-III in Maltese and Turkish subjects PMID: 19234463
    47. Kindlin-3 is more critical to LFA-1 than to VLA-4-adhesive functions in human lymphocytes. PMID: 19617577

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  • 相关疾病：
    Leukocyte adhesion deficiency 3 (LAD3)
  • 亚细胞定位：
    Cell projection, podosome.
  • 蛋白家族：
    Kindlin family
  • 组织特异性：
    Highly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies.
  • 数据库链接：

    HGNC: 23151

    OMIM: 607901

    KEGG: hsa:83706

    STRING: 9606.ENSP00000279227

    UniGene: Hs.180535