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  4. Recombinant Human Fibulin-5 (FBLN5)

Recombinant Human Fibulin-5 (FBLN5)

  • 货号：
    CSB-YP866209HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP866209HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP866209HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP866209HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP866209HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ADCL2; ARCL1A; ARMD3; Dance; Developmental arteries and neural crest EGF like protein; Developmental arteries and neural crest EGF-like protein; EVEC; Fbln5; FBLN5_HUMAN; FIBL 5; FIBL-5; Fibulin-5; FLJ90059; UP50; Urine p50 protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-448
  • 氨基酸序列
    QAQCTNG FDLDRQSGQC LDIDECRTIP EACRGDMMCV NQNGGYLCIP RTNPVYRGPY SNPYSTPYSG PYPAAAPPLS APNYPTISRP LICRFGYQMD ESNQCVDVDE CATDSHQCNP TQICINTEGG YTCSCTDGYW LLEGQCLDID ECRYGYCQQL CANVPGSYSC TCNPGFTLNE DGRSCQDVNE CATENPCVQT CVNTYGSFIC RCDPGYELEE DGVHCSDMDE CSFSEFLCQH ECVNQPGTYF CSCPPGYILL DDNRSCQDIN ECEHRNHTCN LQQTCYNLQG GFKCIDPIRC EEPYLRISDN RCMCPAENPG CRDQPFTILY RDMDVVSGRS VPADIFQMQA TTRYPGAYYI FQIKSGNEGR EFYMRQTGPI SATLVMTRPI KGPREIQLDL EMITVNTVIN FRGSSVIRLR IYVSQYPF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. Stabilizes and organizes elastic fibers in the skin, lung and vasculature. Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling. May act as an adapter that mediates the interaction between FBN1 and ELN.
  • 基因功能参考文献：
    1. study reports the first Chinese family with c.1117C>T mutation of FBLN5; findings confirm a novel subtype of autosomal-dominant Charcot-Marie-Tooth disease due to a mutation in the FBLN5 gene PMID: 28332470
    2. The results showed a significant upregulation of ECM1 and ITGB3, and a significant downregulation for FBLN5 in pelvic organ prolapse patients. PMID: 29729708
    3. Serum fibulin-5 concentration can be regarded as a biomarker for evaluating disease severity and predicting prognosis in intracerebral hemorrhage patients. PMID: 27106135
    4. Fibulin-5 expression is increased in the induced sputum of asthmatic patients. PMID: 28942149
    5. Data show that fibulin-5 strongly binds to the endothelial cell surface reducing endothelial cell viability and interfering with the signaling pathways of the Ang-1/TIE-2 receptor axis. PMID: 27304216
    6. FBLN5 downregulation in human abdominal aortic aneurysm could contribute to extracellular matrix remodeling induced by the inflammatory component of the disease. PMID: 27692634
    7. Identify a SOX9/HDAC-dependent mechanism involved in the down-regulation of FBLN5 by inflammation in abdominal aortic aneurysms. PMID: 27089918
    8. We also found that Fibulin-5 reduces the level of expression of Ki-67, a nuclear protein associated with cell proliferation. Moreover, reduction in Fibulin-5 expression corresponds to an increase of Ki-67 detection in breast tissue samples PMID: 26891749
    9. study demonstrates the pivotal role of the extracellular protein, fibulin-5, on the adhesion and proliferation of human keloid-derived cells, through binding to integrin beta-1 PMID: 26095157
    10. Lower FBLN-5 expression is an important indicator of poor survival in hepatocellular carcinoma. FBLN-5 inhibits HCC adhesion/motility via integrin-dependent mechanism. PMID: 26494967
    11. FBLN5 mRNA expression is upregulated in response to cAMP-mediated decidualization of primary human endometrial stromal cells, although FBLN5 itself does not enhance decidualization PMID: 26506560
    12. Fibulin-5 is significantly down-regulated in ovarian carcinoma and acts as a tumour suppressor by inhibiting the migration and invasion of ovarian cancer cells. PMID: 26251522
    13. Data indicate that Fbln5 promotes PDAC progression by functioning as a molecular rheostat that modulates cell-ECM interactions to reduce ROS production, and thus tip the balance in favor of tumor cell survival and treatment-refractory disease. PMID: 26577699
    14. Data suggest that fibulin-5 functions as a metastasis suppressor in lung cancer by modulating tumor microenvironment to suppress Wnt/beta-catenin signaling. PMID: 25909283
    15. Fibulin-5 may be implicated in the aetiology of rectal prolapse in a subgroup of young male patients. PMID: 25891043
    16. Fibulin-5 expression is a disease marker of hepatic fibrosis. PMID: 25807371
    17. Loss of FBLN5 expression is associated with glioma. PMID: 25684523
    18. Up-regulation of elastin and fibulin-5 mRNA levels in ICA were strongly correlated with family history of cardiovascular disease when compared to CCA PMID: 25792650
    19. Data suggest that expression of FBLN5 (fibulin 5) and LOXL1 (lysyl oxidase-like protein 1) (but not expression of elastin) is down-regulated in uterosacral ligaments of postmenopausal women with pelvic organ prolapse. PMID: 22487196
    20. These results indicate that Fibulin-5 inhibits hepatocellular carcinoma invasion and metastasis by suppressing matrix metalloproteinase-7 expression. PMID: 25494879
    21. Fibulin-5 plays critical roles in proliferation, migration and invasion of certain tumors, and the effect of fibulin-5 on tumorigenesis appears to be largely context-dependent. (Review) PMID: 25845228
    22. Current data provide...strong evidence that common single-nucleotide polymorphisms of the FBLN5 gene are associated with pelvic organ prolapse especially after pelvic floor injury. PMID: 24917111
    23. We identified a clear gene signature for elastogenesis in COPD and propose FBLN5 as a novel player in tissue repair in COPD. PMID: 24990664
    24. Overall the results indicate that LTBP-2 may have a negative regulatory role during elastic fiber assembly, perhaps in displacing elastin microassemblies from complexes with fibulin-5 and/or cell surface heparan sulfate proteoglycans. PMID: 24148803
    25. fibulin-5 overexpression in NPC specimens correlated with advanced tumor metastasis indicating a poor 5-year overall survival PMID: 24386352
    26. Solid phase binding assays detected strong calcium-dependent binding of the short fibulins to immobilized heparin, suggesting that these fibulins may bind cell surface-located heparan sulfate PMID: 23782690
    27. Data indicate mutations of FBLN4, FBLN5, and LTBP4 in 12 probands presenting with type 1 recessive cutis laxa. PMID: 22829427
    28. fibulin-5 is reduced in patients with primary spontaneous pneumothorax who are younger than 25 years of age. PMID: 22790990
    29. Altered expression of miR-200c may have a significant impact on the outcome of leiomyomas growth, maintenance of their mesenchymal and fibrotic characteristics, and possibly their associated symptoms. PMID: 22685266
    30. suggest that uPA promotes cell migration by binding to fibulin-5, initiating its cleavage by plasmin, which leads to its dissociation from beta1-integrin and thereby unblocks the capacity of integrin to facilitate cell motility PMID: 22280367
    31. Data show that the growth rate of the fibulin-5 transfected GFP-F5 cells was remarkably reduced than that of the untransfected cells. PMID: 19767220
    32. The expression of EVEC in ovarian cancer and its metastatic sites was remarkably decreased. PMID: 21122382
    33. The decreased expression of elastin and fibulin-5 was correlated with degree of pelvic organ prolapse. PMID: 19957551
    34. Role of fibulin-5 in metastatic organ colonization PMID: 21454378
    35. This study identifies fibulin-5 as a gene involved in Charcot-Marie-Tooth neuropathies and reveals heterozygous fibulin-5 mutations in 2% of our patients with age-related macular degeneration and hyperelastic skin. PMID: 21576112
    36. MMP9 and fibulin-5 have roles in progression of pelvic organ prolapse in mice and humans PMID: 21519142
    37. spatiotemporal shift from the trophoblast compartment in first trimester to the villous vasculature at term suggests a dual role of fibulin-5 in human placental development. PMID: 21290250
    38. HIF-1 signaling underlies the increase of FBLN5 expression elicited by hypoxia in endothelial cells suggesting that FBLN5 induction could be involved in the adaptive survival response of endothelial cells to hypoxia PMID: 21193390
    39. FIB-5 expression is decreased in uterosacral ligaments of women with uterine prolapse. PMID: 19862539
    40. genetic variation within FBLN5 is unlikely to play any role in the development of abdominal aortic aneurysm PMID: 20133342
    41. Biophysical characterisation of fibulin-5 proteins associated with disease PMID: 20599547
    42. Fibulin-5, a secreted extracellular matrix protein, was identified as a binding partner of Nogo-B. PMID: 20599731
    43. The results identified structural differences for the disease-causing cutis laxa mutants and for one AMD (age-related macular degeneration) variant (G412E), suggesting that this may also be pathogenic. PMID: 20007835
    44. Direct interaction of the extracellular matrix protein DANCE with apolipoprotein(a) mediated by the kringle IV-type 2 domain. PMID: 12111551
    45. Genetic heterogeneity of cutis laxa exists due to a heterozygous tandem duplication in the FBLN5 gene. PMID: 12618961
    46. Missense mutations in the fibulin 5 gene were found in 1.7 percent of patients with AMD. PMID: 15269314
    47. EcSOD-fibulin-5 interaction is needed for ecSOD binding to vascular tissues, regulating their O2*- levels. This is a new mechanism for controlling vascular redox state in the extracellular space in cardiovascular diseases with high oxidative stress. PMID: 15528465
    48. Decreased fibulin-5 may contribute to the pathogenesis of aortic dissection by impairing elastic fiber assembly. PMID: 16153447
    49. The interaction of elastin and fibulin-5 alleles results in elastic fibers susceptible to inflammatory destruction. PMID: 16374472
    50. These studies demonstrate that induction of fibulin 5 gene expression in lung fibroblasts is mediated via canonical TGF-beta/Smad signaling and requires the PI3-kinase/Akt pathway. PMID: 16837650

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  • 相关疾病：
    Neuropathy, hereditary, with or without age-related macular degeneration (HNARMD); Cutis laxa, autosomal dominant, 2 (ADCL2); Cutis laxa, autosomal recessive, 1A (ARCL1A); Macular degeneration, age-related, 3 (ARMD3)
  • 亚细胞定位：
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibulin family
  • 组织特异性：
    Expressed in skin fibroblasts (at protein level)(PubMed:17035250). Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes
  • 数据库链接：

    HGNC: 3602

    OMIM: 219100

    KEGG: hsa:10516

    STRING: 9606.ENSP00000345008

    UniGene: Hs.332708