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  4. Recombinant Human Ficolin-1 (FCN1)

Recombinant Human Ficolin-1 (FCN1)

  • 货号：
    CSB-YP008550HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP008550HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP008550HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP008550HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP008550HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FCN1
  • Uniprot No.：
  • 别名：
    Collagen/fibrinogen domain containing protein 1 ; Collagen/fibrinogen domain-containing protein 1; Fcn1; FCN1_HUMAN; FCNM ; ficolin (collagen/fibrinogen domain containing) 1; Ficolin 1; Ficolin A; Ficolin alpha; Ficolin-1; Ficolin-A; Ficolin-alpha; M ficolin; M-ficolin
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    30-326
  • 氨基酸序列
    A DTCPEVKVVG LEGSDKLTIL RGCPGLPGAP GPKGEAGVIG ERGERGLPGA PGKAGPVGPK GDRGEKGMRG EKGDAGQSQS CATGPRNCKD LLDRGYFLSG WHTIYLPDCR PLTVLCDMDT DGGGWTVFQR RMDGSVDFYR DWAAYKQGFG SQLGEFWLGN DNIHALTAQG SSELRVDLVD FEGNHQFAKY KSFKVADEAE KYKLVLGAFV GGSAGNSLTG HNNNFFSTKD QDNDVSSSNC AEKFQGAWWY ADCHASNLNG LYLMGPHESY ANGINWSAAK GYKYSYKVSE MKVRPA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage.
  • 基因功能参考文献：
    1. M-ficolin interacts with A. fumigatus through interaction with chitin and beta-1,3 glucan and thereby mediates complement activation and potentiates IL-8 secretion of A549 cells. PMID: 28060571
    2. higher ficolin-1 levels were associated with brain ischemic lesions and vasospasm following subarachnoid hemorrhage. PMID: 27734336
    3. in northeastern Brazilian children and adolescents, FCN1 rs1071583 SNP was correlated with earlier age of type 1 diabetes mellitus (T1D) diagnosis; the SNP combination rs2989727 and rs1071583 was involved with T1D protection PMID: 27994205
    4. Ficolin-1 could provide an alternative receptor-mediated mechanism for enhancing Ebola Virus infection, thereby contributing to viral subversion of the host innate immune system. PMID: 26984723
    5. systemic lupus erythematosus patients with high ficolin-1 plasma levels had an increased risk of end-stage renal disease; there was no significant association between ficolin-1 and ficolin-3 with lupus nephritis PMID: 27981461
    6. Ficolin-1 was decreased in patients compared with controls measured at 6 h (median 0.13 vs 0.33 mug/ml, respectively, p < 0.0001). At 48 h, ficolin-1 was significantly higher PMID: 26792363
    7. Data indicate differences in the plasma concentrations of collectin liver 1 and collectin kidney 1, M-ficolin and H-ficol in systemic lupus erythematosus (SLE) patients compared to a group of healthy controls. PMID: 26154564
    8. This study aims to investigate whether an association exists between the ficolins that are part of the lectin complement pathway and systemic lupus erythematosus. PMID: 25069872
    9. the differential binding of ficolin-1 to lymphocyte subsets suggests ficolin-1 as a novel link between innate and adaptive immunity. PMID: 24161415
    10. In patients with early rheumatoid arthritis, elevated plasma M-ficolin levels correlated with a high disease activity score at baseline and 1 year. A low M-ficolin level was the strongest predictor of remission and low disease activity. PMID: 24022747
    11. Data show that a photodynamic therapy (PDT) dose-dependent upregulation of CRP gene, as well as of PTX3 and ficolin 1 genes in lung tumor A549 cells, and indicate critical role played by PI3K/Akt/AP-1 pathway. PMID: 23182717
    12. These results demonstrate that ficolin-1 and PTX3 heterocomplex formation acts as a noninflammatory "find me and eat me" signal to sequester altered-host cells. PMID: 23817411
    13. M-ficolin bound strongly to serotype 19B and 19C polysaccharides. PMID: 23184524
    14. Study interlinks the genotype and phenotype relationship concerning polymorphisms in FCN1 and corresponding concentrations and biological functions of M-ficolin. PMID: 23209787
    15. A genotype study of common promoter FCN1 single nucleotide polymorphisms (SNPs) finds evidence for opposite associations between different FCN1 haplotypes and clinical leprosy in Euro-Brazilians and Afro-Brazilians. PMID: 22941510
    16. Two polymorphisms in the promoter region of FCN1 is associated with the expression and synthesis of ficolin-1 as well as associated with decreased survival in a cohort of patient with systemic inflammatory response syndrome and sepsis. PMID: 22673311
    17. M-ficolin concentrations in cord blood are related to circulating phagocytes and to early-onset sepsis PMID: 22391637
    18. In patients with haematological malignancy undergoing chemotherapy, those with high MASP-2 were less likely to develop severe infections. PMID: 22236007
    19. Data identified the binding interface between CRP and FBG, locating it to the pH- and calcium-sensitive C-terminal region of FBG PMID: 21689722
    20. It is postulated that the elevation of concentration of the two components of the leptin pathway, L-ficolin and MASP-2, may compensate for the insufficient activity of the LP in mannose-binding lectin deficiency PMID: 21974696
    21. serum levels associate with neutrophil and monocyte counts in blood and bone marrow from children with cancer PMID: 21112665
    22. data indicate interaction of M-ficolin with PTX3 arises from its ability to bind sialylated ligands; M-ficolin-PTX3 interaction described represents a novel case of cross-talk between soluble pattern-recognition molecules PMID: 21490156
    23. M-ficolin collaborates with its interacting partners (G protein-coupled receptor 43 and C-reactive protein) to initiate, mediate, and regulate the immune response during bacterial infection. PMID: 21037097
    24. We suggest that M-ficolin should also be considered as a humoral pattern recognition molecule. PMID: 20375634
    25. a novel self-recognition mechanism of leukocytes mediated by the fibrinogen-like domain of Ficolin-1. PMID: 20400674
    26. sialic acid binding specificity of M-ficolin, emphasizing the essential role of Tyr(271) in this respect PMID: 20032467
    27. Results describe the x-ray structure of human mannan-binding lectin-associated protein 19 (MAp19), and identify the residues involved in the interaction of MAp19 with mannan-binding lectin and L-ficolin. PMID: 15117939
    28. M-ficolin is present on the surface of peripheral blood monocytes and binds to acetylated compounds. PMID: 16305643
    29. The crystal structure and ligand binding study of human M-ficolin reveal how the three fibrinogen domains form a trimer and provide a structural basis for understanding how ficolins discriminate between self and non-self. PMID: 17148457
    30. M-Ficolin expression is silenced in macrophages but can be re-activated after prolonged activation via TLR2 and TLR4 PMID: 17928056
    31. This study was conducted to determine mannose-binding lectin (MBL)/MBL-associated serine protease (MASP) complexes and L-ficolin/MASP complexes bind to different strains of group B streptococci to activate the lectin pathway. PMID: 17938215
    32. FCN1 GENE POLYMORPHISM IS ASSOCIATED WITH THE DEVELOPMENT OF RHEUMATOID ARTHITIS. PMID: 18032536
    33. Ficolin-1 exists in human plasma and serum under normal conditions, hereby revising the general assumption that Ficolin-1 is solely a cellular associated protein. PMID: 18343499
    34. Data show that low cord blood M-ficolin was associated with higher NEC-associated fatality and with increased need for mechanical ventilation, and future studies need to assess whether M-ficolin is involved in multiorgan failure and pulmonary disease. PMID: 19539995
    35. Ficolin-1 is present in a highly mobilizable subset of human neutrophil granules and associates with the cell surface after stimulation with fMLP. PMID: 19741154
    36. CRP binding is enhanced by conformational bending at the neck region of M-ficolin, to avoid steric hindrance by the COL domain. PMID: 19853918

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  • 亚细胞定位：
    Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Note=Found on the monocyte and granulocyte surface (PubMed:20400674).
  • 蛋白家族：
    Ficolin lectin family
  • 组织特异性：
    Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes.
  • 数据库链接：

    HGNC: 3623

    OMIM: 601252

    KEGG: hsa:2219

    STRING: 9606.ENSP00000360871

    UniGene: Hs.440898