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  4. Recombinant Human Filaggrin (FLG), partial

Recombinant Human Filaggrin (FLG), partial

In Stock
  • 货号：
    CSB-YP008712HU
  • 规格：
    ￥1500
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    ATOD2; Epidermal filaggrin; FILA_HUMAN; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    Yeast
  • 分子量：
    26.8kDa
  • 表达区域：
    3838-4061aa
  • 氨基酸序列
    DSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Recombinant Human Filaggrin/FLG is a partial-length protein expressed with an N-terminal 6xHis-tag in the yeast. Its expression region corresponds to 3838-4061aa of human FLG protein. Its purity was determined by SDS-PAGE and reached up to 90%. This recombinant FLG protein may be used to synthesize antibodies against FLG or on the studies of FLG-associated signal transduction. FLG proteins in-stock are available.

    FLG is an epidermal differentiation protein that plays a fundamental role in the development and maintenance of the skin barrier. The binding of FLG with the intermediate keratin filaments leads to their aggregation and compaction, making cells collapse and flattening that is required for squame biogenesis. Within the squames, FLG undergoes further proteolysis and degrade into hygroscopic amino acids and other derivatives, which make up the natural moisturizing factor (NMF) responsible for water retention in the stratum corneum. FLG mutations perturb the skin barrier function, which causes ichthyosis vulgaris, increase the risk of atopic dermatitis and other atopic diseases.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • 基因功能参考文献：
    1. The present study demonstrated that the downregulation of filaggrin in the epidermis by toluene is mediated by ERK1/2 and STAT3-dependent pathways. PMID: 27498358
    2. FLG mutation was not associated with MM in the studied populations. PMID: 28833578
    3. The results show that patients carrying filaggrin mutations had increase prevalence of Staphylococcus aureus colonization. PMID: 28317091
    4. We suggest that SNP in FLG (rs11204981) may serve as an important predictive marker for the combined eczema plus asthma phenotype, and that the highest level of expression in heterozygous may have a protective role in developing allergy phenotype. PMID: 29569866
    5. This study indicates an increased susceptibility to actinic keratosis in individuals with homozygous, but not heterozygous, FLG mutations and in patients with atopic dermatitis compared to psoriasis. PMID: 28213896
    6. This study demonstrated, for the first time, that FLG expression in UCB is associated with eczema development in infancy. Moreover, our analysis provided prediction models that were capable of discriminating, to a great extent, between those who will and will not develop eczema in infancy. PMID: 28502108
    7. In a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC. both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations PMID: 27805415
    8. FLG and POSTN expression may be downregulated and upregulated, respectively, in the esophageal mucosa of patients with active eosinophilic esophagitis, and these changes may be restored with treatment in a significant percentage of cases. PMID: 28644349
    9. immunoreactivity for filaggrin was significantly more intense in the oral mucosa in the patients with OLP/OLL compared with healthy controls PMID: 28000306
    10. atopic dermatitis patients without palmoplantar hyperlinearity unlikely to carry FLG mutations PMID: 27120251
    11. FLG mutations are strongly associated with atopic eczema and confer a significant risk of allergic sensitization and asthma in the context of eczema in Polish children. PMID: 29068602
    12. There was no association of the atopy risk variants in the FLG gene with OFG. PMID: 27306066
    13. suggest that FLG P478S is a kind of disease modifier which affects serologic parameters such as EDN and ECP PMID: 29281699
    14. In this study of 97 174 individuals from the Danish general population, FLG loss-of-function mutations were associated with increased ischemic stroke risk PMID: 28164424
    15. study found that among patients with atopic dermatitis (AD), common FLG null mutations are associated with earlier AD onset in a dose-dependent manner, whereas TSLP rs1898671 appears unrelated to the timing of AD onset PMID: 28479194
    16. The two SNPs K4671X and rs3126085 of FLG were related to Epstein-Barr virus (EBV)-associated gastric carcinoma. PMID: 28455573
    17. FLG mutation is associated with IgE sensitization to peanut but not to other allergens in Swedish children up to 16 years of age PMID: 28456621
    18. Erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo. PMID: 28358172
    19. FLG mutations are associated with early onset of atopic dermatitis, more severe clinical course of disease, and a significantly increased risk ofMolluscum contagiosum sustained skin infection PMID: 28866311
    20. Study conducted in Croatia found a low frequency of FLG null-mutations in general population (2.6%) and did not confirm FLG null-mutations as an etiological factor for Atopy and Atopic disease in the studied population. PMID: 29087092
    21. women with FLG mutations may have an increased risk of AD flares during pregnancy and of enduring postpartum problems attributable to perineal trauma during delivery. PMID: 26835886
    22. FLG mutations are risk factors for atopic dermatitis in Finns, but disease severity and treatment response were independent of patient FLG status. PMID: 27840886
    23. Patients with both atopic dermatitis and common filaggrin gene mutations are more frequently affected by reduced health-related quality of life PMID: 27995642
    24. Multiple lines of evidence suggest that FLG genetic variation, have little or no effect on fitness in modern humans. Haplotype-level analysis revealed a recent selective sweep which increased the allele frequency of the Huxian haplogroup in Asian populations. PMID: 27678121
    25. FLG mutations lead to alterations in epidermal eicosanoid metabolism in atopic dermatitis patients PMID: 27793761
    26. Filaggrin gene mutations are risk factors for the presence and persistence of atopic dermatitis and explain the discordance of atopic dermatitis within dizygotic twin pairs. PMID: 27653621
    27. Variations in FLG and TSLP genotype were associated with differences in self-reported skin clearance, TCI usage, and steroid usage. PMID: 27902816
    28. We show that subjects with FLG-null mutations have more mature Langerhans cells in nonlesional skin irrespective of whether they have AD. PMID: 26934939
    29. we have proposed that this latitude-dependent gradient of FLG mutations across Europe, Asia and Africa could have provided an evolutionary advantage for heterozygous FLG mutation carriers, residing at northern latitudes, depletion of the FLG downstream product, trans-urocanic acid, would facilitate the intracutaneous synthesis of vitamin D3 by allowing increased transcutaneous absorption of UVB photons PMID: 28338939
    30. The results of the present study demonstrate that filaggrin knockdown inhibits NHEK migration, adhesion and proliferation, promotes apoptosis and disturbs cell cycle progression. PMID: 27485743
    31. FLG rs2065955 polymorphism was significantly related to gastric carcinoma. PMID: 27535066
    32. Four FLG null mutations (3321delA, K4022X, S3296X, and S2889X) were identified in Korean patients with Atopic Dermatitis PMID: 28120571
    33. IL-33, which is a representative Th2 cytokine, affect as a crucial factor of skin barrier dysfunction by reducing FLG expression in human keratinocytes. PMID: 26867960
    34. The s data reveals that the CNV of FLG is associated with AD development in Koreans. PMID: 26554544
    35. Filaggrin Gene Mutation c.3321delA is Associated with Dry Phenotypes of Atopic Dermatitis in the Chinese Han Population PMID: 27270549
    36. A mutation p.Y1767X, was related to early onset and severe symptoms, which last until adulthood, and severe atopic dermatitis with other atopic diseases, such as asthma. PMID: 27366014
    37. The present study examined the possible relationship between SNPs of FLG and chronic idiopathic urticaria (CIU) for the first time, and demonstrated that none of five investigated SNPs (rs2485518, rs3126065, rs2786680, rs3814300, and rs3814299) are correlated with CIU in an Iranian population. PMID: 26796858
    38. The study revealed 66 FLG mutation carriers and demonstrated an association between c.2282del4 deletion and atopic dermatitis development in Russians and Tatars of Volga-Ural region of Russia. PMID: 27363669
    39. The levels of filaggrin, inflammatory T helper 2 polarizing cytokines (thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL-33)) and chemokine (C-C motif) ligand 27 (CCL27), histological severity markers, T and dendritic cell counts in biopsies from lesional skin of severe atopic dermatitis patients with and without filaggrin mutation and healthy skin were quantified. No significant differences were found. PMID: 26536977
    40. The imbalance between Th1 and Th2 polarized immune response seems to extend to Filaggrin homeostasis, through the network of Filaggrin processing enzymes. PMID: 26831231
    41. Levels of FLG, FLG2 and SPRR3 mRNAs and proteins were reduced in AD skin. PMID: 27304082
    42. Data suggest that the c.1360A>G (p.T454A) and c.10363G>T (p.D3455Y) mutations of the filaggrin (FLG) gene may lead to alteration of the structure and function of the FLG protein and cause ichthyosis vulgaris in the two families. PMID: 27577213
    43. genotype-phenotype correlation not obvious in Korean atopic dermatitis patients with null mutations PMID: 25997159
    44. Results show a strong association of FLG loss-of-function mutations was found with doctor-diagnosed CD and to a lesser extent also with self-reported CD, but not with respiratory symptoms or atopy. PMID: 26451970
    45. Identify acefylline as an activator of peptidylarginine deiminase 1 and 3 in the epidermis, resulting in filaggrin deimination. PMID: 26616205
    46. FLG has been, and still remains the major susceptibility gene in patients with AD. PMID: 25580797
    47. Letter: Knockdown of either filaggrin or loricrin increases the productions of interleukin (IL)-1alpha, IL-8, IL-18 and granulocyte macrophage colony-stimulating factor in stratified human keratinocytes. PMID: 26381575
    48. Supplementation with the PPARalpha agonist WY14643 improved the homeostasis and barrier function of filaggrin deficient skin models by normalization of the free fatty acid profile. PMID: 26472199
    49. Filaggrin genotype does not determine the skin's threshold to UV-induced erythema. PMID: 26830116
    50. In Iranian patients, this study demonstrated that there was no significant association between polymorphisms of FLG gene variants and AD. PMID: 25230061

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  • 相关疾病：
    Ichthyosis vulgaris (VI); Dermatitis atopic 2 (ATOD2)
  • 亚细胞定位：
    Cytoplasmic granule.
  • 蛋白家族：
    S100-fused protein family
  • 组织特异性：
    Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal
  • 数据库链接：

    HGNC: 3748

    OMIM: 135940

    KEGG: hsa:2312

    STRING: 9606.ENSP00000357789

    UniGene: Hs.654510