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  4. Recombinant Human G-rich sequence factor 1 (GRSF1)

Recombinant Human G-rich sequence factor 1 (GRSF1)

  • 货号：
    CSB-YP615535HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP615535HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP615535HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP615535HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP615535HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GRSF1
  • Uniprot No.：
  • 别名：
    G rich RNA sequence binding factor 1; G rich sequence binding factor 1; G-rich sequence factor 1; GRSF-1; GRSF1; GRSF1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    118-480
  • 氨基酸序列
    SQE SKTTYLEDLP PPPEYELAPS KLEEEVDDVF LIRAQGLPWS CTMEDVLNFF SDCRIRNGEN GIHFLLNRDG KRRGDALIEM ESEQDVQKAL EKHRMYMGQR YVEVYEINNE DVDALMKSLQ VKSSPVVNDG VVRLRGLPYS CNEKDIVDFF AGLNIVDITF VMDYRGRRKT GEAYVQFEEP EMANQALLKH REEIGNRYIE IFPSRRNEVR THVGSYKGKK IASFPTAKYI TEPEMVFEEH EVNEDIQPMT AFESEKEIEL PKEVPEKLPE AADFGTTSSL HFVHMRGLPF QANAQDIINF FAPLKPVRIT MEYSSSGKAT GEADVHFETH EDAVAAMLKD RSHVHHRYIE LFLNSCPKGK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA.
  • 基因功能参考文献：
    1. Deletion of either of the three GRSF1 quasi-RNA-recognition motifs (qRRMs) severely impairs RNA-binding. PMID: 29288125
    2. Data show that some of the naturally occurring guanine-rich RNA sequence binding factor 1 (GRSF1) mutants exhibited a strongly reduced RNA-binding activity although the general protein structure was hardly affected. PMID: 29366917
    3. HuR and GRSF1 govern the cytoplasmic and mitochondrial localization of the lncRNA RMRP, which is encoded by nuclear DNA but has key functions in mitochondria. PMID: 27198227
    4. MiR-346 depends on GRSF1 to upregulate another target gene. PMID: 26507454
    5. Grsf1 controls translation of the protein Use1, possibly by positioning the 40S ribosomal subunit and associated translation factors in front of the translation start site. PMID: 25184340
    6. The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression. PMID: 23473033
    7. GRSF1 regulates RNA processing in mitochondrial RNA granules. PMID: 23473034
    8. GRSF-1 functions by selectively recruiting cellular and viral mRNAs containing 5' UTR GRSF-1 binding sites to polyribosomes, which is mediated through interactions with cellular proteins PMID: 12239318

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  • 亚细胞定位：
    [Isoform 1]: Mitochondrion matrix.; [Isoform 2]: Cytoplasm.
  • 数据库链接：

    HGNC: 4610

    OMIM: 604851

    KEGG: hsa:2926

    STRING: 9606.ENSP00000254799

    UniGene: Hs.309763