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  4. Recombinant Human Group 3 secretory phospholipase A2 (PLA2G3)

Recombinant Human Group 3 secretory phospholipase A2 (PLA2G3)

  • 中文名称：
    人PLA2G3重组蛋白
  • 货号：
    CSB-YP882150HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PLA2G3重组蛋白
  • 货号：
    CSB-EP882150HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PLA2G3重组蛋白
  • 货号：
    CSB-EP882150HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PLA2G3重组蛋白
  • 货号：
    CSB-BP882150HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PLA2G3重组蛋白
  • 货号：
    CSB-MP882150HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    EC 3.1.1.4; GIII sPLA2; Group 3 secretory phospholipase A2; Group III secretory phospholipase A2; PA2G3_HUMAN; Phosphatidylcholine 2 acylhydrolase GIII; Phosphatidylcholine 2-acylhydrolase 3; phospholipase A2 group III ; PLA2G3; sPLA2-III; SPLA2III
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-509
  • 氨基酸序列
    G SPALRWYRTS CHLTKAVPGN PLGYLSFLAK DAQGLALIHA RWDAHRRLQS CSWEDEPELT AAYGALCAHE TAWGSFIHTP GPELQRALAT LQSQWEACRA LEESPAGARK KRAAGQSGVP GGGHQREKRG WTMPGTLWCG VGDSAGNSSE LGVFQGPDLC CREHDRCPQN ISPLQYNYGI RNYRFHTISH CDCDTRFQQC LQNQHDSISD IVGVAFFNVL EIPCFVLEEQ EACVAWYWWG GCRMYGTVPL ARLQPRTFYN ASWSSRATSP TPSSRSPAPP KPRQKQHLRK GPPHQKGSKR PSKANTTALQ DPMVSPRLDV APTGLQGPQG GLKPQGARWV CRSFRRHLDQ CEHQIGPREI EFQLLNSAQE PLFHCNCTRR LARFLRLHSP PEVTNMLWEL LGTTCFKLAP PLDCVEGKNC SRDPRAIRVS ARHLRRLQQR RHQLQDKGTD ERQPWPSEPL RGPMSFYNQC LQLTQAARRP DRQQKSWSQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids without apparent head group selectivity. Contributes to phospholipid remodeling of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles. Hydrolyzes LDL phospholipids releasing unsaturated fatty acids that regulate macrophage differentiation toward foam cells. May act in an autocrine and paracrine manner. Secreted by immature mast cells, acts on nearby fibroblasts upstream to PTDGS to synthesize prostaglandin D2 (PGD2), which in turn promotes mast cell maturation and degranulation via PTGDR. Secreted by epididymal epithelium, acts on immature sperm cells within the duct, modulating the degree of unsaturation of the fatty acyl components of phosphatidylcholines required for acrosome assembly and sperm cell motility. Facilitates the replacement of fatty acyl chains in phosphatidylcholines in sperm membranes from omega-6 and omega-9 to omega-3 polyunsaturated fatty acids (PUFAs). Coupled to lipoxygenase pathway, may process omega-6 PUFAs to generate oxygenated lipid mediators in the male reproductive tract. At pericentrosomal preciliary compartment, negatively regulates ciliogenesis likely by regulating endocytotic recycling of ciliary membrane protein. Coupled to cyclooxygenase pathway provides arachidonate to generate prostaglandin E2 (PGE2), a potent immunomodulatory lipid in inflammation and tumorigenesis. At colonic epithelial barrier, preferentially hydrolyzes phospholipids having arachidonate and docosahexaenoate at sn-2 position, contributing to the generation of oxygenated metabolites involved in colonic stem cell homeostasis. Releases C16:0 and C18:0 lysophosphatidylcholine subclasses from neuron plasma membranes and promotes neurite outgrowth and neuron survival.
  • 基因功能参考文献：
    1. Several hits were discovered, and data have been uploaded to PubChem. This study describes the first high-throughput optical screening assay for secreted phospholipase A2 inhibitors based on a phospholipid vesicle substrate PMID: 27146384
    2. Studies indicate that the expression of secreted phospholipases A2 (sPLA2s), most notably the group IIA, III and X enzymes, is dysregulated in various malignant tissues. PMID: 25286228
    3. sPLA2GIII expression may be used as a risk factor for lymph node metastasis and a prognostic marker in colorectal cancer. In addition, sPLA2GIII and sPLA2GX may play opposing roles in colorectal carcinogenesis PMID: 25964585
    4. Data indicate that the expression of genes encoding hGIIA, hGIII and hGX sPLA2s (PLA2G2A, PLA2G3 and PLA2G10, respectively) in breast tumour biopsies differs from that in normal tissues. PMID: 24508801
    5. we describe that PLA2G3 gene silencing produced a marked inhibition of xanthine/xanthine oxidase induced cell death, and that PLA2G3 polymorphisms are associated with Alzheimer disease in a Spanish case-control sample. PMID: 20930276
    6. The availability of the human group III phospholipase A(2) (hIIIPLA(2)) amino acid sequence offers an opportunity to study its structural features by modeling. PMID: 20654644
    7. Secreted PLA2 III induces apoptosis of primary cortical neurons in culture by suppressing production of Bcl-xl, promoting Bax, augmenting caspase 3 and cleaving alpha-fodrin. PMID: 20492356
    8. Lys 43 and Asp 46 in rhUG are critical residues for the sPLA2-inhibitory activity of UG PMID: 12127976
    9. cellular arachidonate (AA) release and prostaglandin (PG) production are regulated by novel classes of secretory phospholipase A(2)s (sPLA(2)s), groups III and XII PMID: 12522102
    10. sPLA(2)-III may have a role in cancer development by stimulating tumor cell growth and angiogenesis PMID: 15863501
    11. potential contribution of sPLA2-III to neuronal differentiation and its function under certain conditions PMID: 17868035
    12. the biological action of group III sPLA(2) depends on its N-terminal domain PMID: 17980167
    13. a potential functional link between PLA2G3 and atherosclerosis, as has recently been proposed for PLA2G5 and PLA2G10. PMID: 18801741
    14. This study showed that endogenous group III sPLA2 (PLA2G3) is expressed in mouse skin and that transgenic mice overexpressing human PLA2G3 spontaneously develop skin inflammation. PMID: 19371233

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  • 亚细胞定位：
    Secreted. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Recycling endosome.
  • 蛋白家族：
    Phospholipase A2 family
  • 组织特异性：
    Expressed in kidney, heart, liver, and skeletal muscle. Also present in placenta and peripheral blood leukocytes. Not detected in colon, thymus, spleen and small intestine. In lung, expressed in bronchial epithelial cells and alveolar macrophages, but sca
  • 数据库链接：

    HGNC: 17934

    OMIM: 611651

    KEGG: hsa:50487

    STRING: 9606.ENSP00000215885

    UniGene: Hs.149623