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  4. Recombinant Human Growth arrest and DNA damage-inducible protein GADD45 alpha (GADD45A)

Recombinant Human Growth arrest and DNA damage-inducible protein GADD45 alpha (GADD45A)

  • 货号：
    CSB-EP009161HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    DDIT 1; DDIT-1; DDIT1; DNA damage inducible transcript 1; DNA damage-inducible transcript 1 protein; GA45A_HUMAN; GADD45; GADD45A; Growth arrest and DNA damage inducible 45 alpha ; Growth arrest and DNA damage inducible alpha; Growth arrest and DNA damage-inducible protein GADD45 alpha
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    34.3kDa
  • 表达区域：
    1-165aa
  • 氨基酸序列
    MTLEEFSAGEQKTERMDKVGDALEEVLSKALSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVALQIHFTLIQAFCCENDINILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPER
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
  • 基因功能参考文献：
    1. Human miR-374 induces apoptosis and inhibits proliferation, migration, and invasion of human squamous carcinoma cells through P53 signaling pathway by down-regulating Gadd45a. PMID: 28679648
    2. Findings demonstrated that miR-148a promotes glioma cell invasion and tumorigenesis by downregulating GADD45A. Findings provide novel insights into how GADD45A is downregulated by miR-148a in IDH1R132H glioma PMID: 28445981
    3. A mechanism in which GADD45a is required for demethylation of the MMP-9 promoter and the induction of diabetic wound healing. The inhibition of GADD45a might be a therapeutic strategy for diabetic foot ulcers. PMID: 29244109
    4. Our findings indicate that GADD45 essentially suppresses the MKK7-JNK pathway and suggest that differentially expressed GADD45 family members fine-tune stress-inducible JNK activity. PMID: 29037961
    5. Stimulation of ribosomal RNA gene promoter by transcription factor Sp1 involves active DNA demethylation by Gadd45-NER pathway. PMID: 27156884
    6. the enhancement of GADD45A-mediated base excision repair modulated by ATF2 might be a potential protective mechanism of visible red light. PMID: 27729279
    7. Data show that growth arrest and DNA-damage-inducible 45 alpha protein (Gadd45a) expression is up-regulated in chronic phase myelocytic leukemia, chronic (CML) patient samples but down-regulated in accelerated and blast crisis phase samples. PMID: 28086219
    8. These data revealed that Riboflavin plus ultraviolet (UV) pathogen reduction technology effectively inhibited proliferation and induced apoptosis of lymphocytes by promoting GADD45alpha expression, which subsequently activates p38 and JNK signaling pathways. PMID: 27905125
    9. data suggest GADD45 proteins play a role in controlling HIV-1 transcription and in regulating HIV-1 latency PMID: 26994425
    10. Taken together, our results suggest that ROS generation might be a predisposing event in berberine-induced mitochondrial apoptosis in EBV-transformed B cells through the upregulation of XAF1 and GADD45alpha expression by MAPK and functional p53. PMID: 27121748
    11. Collective results lead us to conclude that GADD45alpha modulates curcumin sensitivity through activation of c-Abl > JNK signaling in a mismatch repair-dependent manner PMID: 26833194
    12. BRCA1 may act as an important negative regulator in cell cycle progression and tumorigenesis through regulating the stability of Smad4; this defines a novel link that connects BRCA1 to TGF-beta1/Smad pathway. PMID: 26022109
    13. MiR-362-5p promotes the malignancy of chronic myelocytic leukaemia via down-regulation of GADD45alpha PMID: 26545365
    14. these data suggest a dual function of GADD45a in oxidative DNA demethylation, to promote directly or indirectly TET1 activity and to enhance subsequent 5-formylcytosine/5-carboxylcytosine removal. PMID: 26546041
    15. These data suggest that GADD45A (1506T>C) is a new tumor susceptibility gene and could be a useful molecular marker for assessing ovarian cancer risk and for predicting ovarian cancer patient prognosis. PMID: 26422378
    16. Data show that heterogeneous nuclear ribonucleoprotein A0 (hnRNPA0) drives chemo-resistance of p53-mutant tumor cells via p27Kip1/Gadd45alpha mRNAs. PMID: 26602816
    17. GADD45A mRNA expression was significantly greater in patients with SLE compared to controls. PMID: 25899090
    18. Report GADD45alpha luciferase reporter assays in human cells for assessing the genotoxicity of environmental pollutants. PMID: 25560476
    19. Data showed that upregulation of cytoplasmic AFP is associated with down-regulation of GADD45a in hepatocellular neoplastic tissues and that AFP mediated downregulation of GADD45a to accelerate aberrant growth of HBV infected cells. PMID: 25846475
    20. Serum GADD45a methylation in combination with PSA and fcDNA level was useful in distinguishing benign from prostate cancer patients. PMID: 26171936
    21. GADD45a promoter regulation by a functional genetic variant is associated with acute mechanical lung injury. PMID: 24940746
    22. Induction of GADD45alpha protects M17 neuroblastoma cells against MPP+. PMID: 25469469
    23. Knockdown of Gadd45a gene abolished the G2/M arrest. PMID: 25450267
    24. High GADD45A expression is associated with response to low intravenous immunoglobulin therapy in patients with Kawasaki disease. PMID: 25449331
    25. results for the first time demonstrate that nucleolin-SUMO at K294R plays a critical role in its nucleus sequestration and gadd45alpha mRNA binding activity. PMID: 25561743
    26. Individuals carrying the GADD45Ars532446 genotype were at higher risk for chromosomal damage compared with the wild type genotype. PMID: 24464562
    27. DNA damage-inducible transcript 1 protein(Gadd45alpha) may be involved in human trophoblast migration and invasion and may function as an important negative regulator at the foetal-maternal interface during early pregnancy PMID: 24755561
    28. Results show TARID binds to the TCF21 promoter and recruits GADD45A and TDG to direct base excision repair for demethylation. PMID: 25087872
    29. the present results revealed, for the first time, that Hes-1 could be SUMO-modified by PIAS1 and GADD45alpha is a novel target of Hes-1 PMID: 24894488
    30. Expression of cell cycle regulatory factors hus1, gadd45a, rb1, cdkn2a and mre11a correlates with expression of clock gene per2 in human colorectal carcinoma tissue. PMID: 24062075
    31. we conclude that repair, together with Gadd45 and MDM2 genes, were involved in light and dark reaction mechanisms. PMID: 24177565
    32. the promotion of base excision repair activity by a p53-dependent pathway is mediated by an interaction between Gadd45a, PCNA and APE1 in the presence of organic selenium that results in increased APE1 activity PMID: 23846616
    33. Gadd45a, encodes a ubiquitously expressed protein that is often induced by DNA damage and other stress signals associated with growth arrest and apoptosis. (Review) PMID: 24104470
    34. The gadd45a gene has both tumor suppressor and tumor promoter functions, dependent on the tissue/cell type and transforming event. (Review) PMID: 24104471
    35. The Gadd45A protein is a stress sensor in preeclampsia. PMID: 24104476
    36. Gadd45a levels are significantly associated with hormone receptor status in human breast cancer PMID: 23706118
    37. The absolute mRNA content of paraffin embedded samples of salivary gland cancer was determined by quantitative reverse transcription-PCR using specific primers for NFkB1, GADD45A and JNK1. PMID: 23603344
    38. Methylation-mediated repression of GADD45A expression is associated with gastric cardia adenocarcinoma. PMID: 23616123
    39. Data indicate that salivary gland epithelial cells (SGEC) demethylation in Sjogren's syndrome (SS)patients was associated with a 7-fold decrease in DNA methyl transferase DNMT1 and a 2-fold increase in Gadd45-alpha expression. PMID: 23478041
    40. GADD45A hyper-methylation may be detecting a broader epigenetic phenotype in acute myeloid leukemia. PMID: 23187294
    41. The expressions of p21(Cip1/WAF1), Gadd45alpha and p53 are associated with the clinicopathologic features and prognosis in breast cancer. PMID: 23158659
    42. Proliferating cell nuclear antigen (PCNA) binding site on Gadd45alpha plays a critical role in modulating the interaction with PCNA and APE1, affecting base excision repair. PMID: 23485469
    43. reduced Gadd45alpha protein expression by forced miR-499 expression indicated it's a diabetes-associated gene which might potentially be involved in both DCM and DM-induced baroreflex dysfunction PMID: 23227140
    44. The expression of Gadd45a in normal, developing brain is tightly regulated. PMID: 22970179
    45. To examine the functions of Gadd45a in cell invasion and metastasis, we performed the adhesion, wound-healing and transwell assays in Gadd45a (+/+) and Gadd45a (-/-) MEF cell lines. PMID: 22825327
    46. Hepatitis C virus NS5A repressed p53 expression, which was followed by a subsequent decrease in GADD45alpha expression, which triggered cellular proliferation. PMID: 23114628
    47. The induction of Gadd45a in response to preeclampsia stresses was associated with the activation of its downstream effectors phospho-p38 and phospho-JNK. Soluble Flt-1 levels were subsequently regulated through one of these effectors, but not both. PMID: 22718299
    48. the effect of APRIL is mediated via BCMA, which does not activate the classical NF-kappaB pathway, whereas it induces a novel signaling pathway, which involves JNK2 phosphorylation, FOXO3A activation, and GADD45 transcription PMID: 23071284
    49. Estrogen receptor beta causes a G2 cell cycle arrest by inactivating CDK1 through the repression of cyclin B1 and stimulation of GADD45A and BTG2 expression. PMID: 21120602
    50. [review] Decreased inducibility of Gadd45 members has far-reaching consequences including genome instability, DNA damage, and disorders in cellular homeostasis, which contribute to the aging process and age-related disorders. PMID: 21986581

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    GADD45 family
  • 数据库链接：

    HGNC: 4095

    OMIM: 126335

    KEGG: hsa:1647

    STRING: 9606.ENSP00000360025

    UniGene: Hs.80409