Recombinant Human Growth factor receptor-bound protein 2 (GRB2)
-
货号:CSB-YP009889HU
-
规格:
-
来源:Yeast
-
其他:
-
货号:CSB-EP009889HU
-
规格:
-
来源:E.coli
-
其他:
-
货号:CSB-EP009889HU-B
-
规格:
-
来源:E.coli
-
共轭:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
-
其他:
-
货号:CSB-BP009889HU
-
规格:
-
来源:Baculovirus
-
其他:
-
货号:CSB-MP009889HU
-
规格:
-
来源:Mammalian cell
-
其他:
产品详情
-
纯度:>85% (SDS-PAGE)
-
基因名:
-
Uniprot No.:
-
别名:Abundant SRC homology ; Adapter protein GRB2; ASH; Ash protein; EGFRBP GRB2; Epidermal growth factor receptor binding protein ; Epidermal growth factor receptor binding protein GRB2; GRB 2; GRB2 adapter protein; Grb2; GRB2_HUMAN; Grb3 3; Growth factor receptor bound protein 2; Growth factor receptor bound protein 3; Growth factor receptor-bound protein 2; HT027 ; MST084; MSTP084; NCKAP2; OTTHUMP00000166096 ; OTTHUMP00000166097; OTTHUMP00000166098; Protein ash; SEM5; SH2/SH3 adapter GRB2
-
种属:Homo sapiens (Human)
-
蛋白长度:Full length protein
-
表达区域:1-217
-
氨基酸序列MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK NYIEMKPHPW FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK FGNDVQHFKV LRDGAGKYFL WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD IEQVPQQPTY VQALFDFDPQ EDGELGFRRG DFIHVMDNSD PNWWKGACHG QTGMFPRNYV TPVNRNV
-
蛋白标签:Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
产品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
复溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
-
储存条件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
-
保质期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
货期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
-
注意事项:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
Datasheet :Please contact us to get it.
相关产品
靶点详情
-
功能:Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.; Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
-
基因功能参考文献:
- that miR-1258 can suppress NSCLC progression by targeting the GRB2/Ras/Erk pathway PMID: 30069987
- The binding site of miR-433-3p was identified in the 3'UTR region of GRB2. Western blotting and FQ-PCR showed that miR-433-3p inhibited the mRNA and protein expression of GRB2. PMID: 29730656
- study unravels a unique role of Grb2 in protecting the cytoskeletal architecture in AD-like conditions and presents a potential new strategy for controlling neurodegeneration PMID: 28360125
- M. tuberculosis-initiated human mannose receptor signaling regulates macrophage recognition and vesicle trafficking by gamma Fc receptors, Grb2, and SHP-1. PMID: 28978467
- Data indicate GRB2 as a direct target of miR-329 in pancreatic cancer cells, and expression of GRB2 was inversely correlated with miR-329 expression in pancreatic cancer patients. PMID: 26885689
- EGFR colocalization with GRB2 as assessed by PLA is not correlated with EGFR expression levels or mutation status, defining a patient group that may show EGFR pathway activation, as illustrated by its prognostic value. PMID: 27449805
- Low GRB2 expression is associated with Breast Cancer. PMID: 23826330
- ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP are potent reference genes in neuroendocrine tumors of the lung. PMID: 27802291
- Following phosphorylation of the tyrosine, the proteins growth factor receptor-bound protein 2 (Grb2), Grb2-related adaptor downstream of Shc (Gads), and p85 subunit of phosphoinositide 3-kinase may bind to pYMNM (where pY is phosphotyrosine) via their Src homology 2 (SH2) domains, leading to downstream signaling to distinct immune pathways. These three adaptor proteins bind to the same site on CD28 with variable affinity PMID: 27927989
- investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation PMID: 27264952
- The study presents EPR spectroscopic analysis of the binding of the wild-type and mutant Grb2 SH2 domains to the CSpYVNVQC peptide variant. These data confirm that the binding specificity of SH2 domains, even that of Grb2, results from multiple factors and does not depend merely on the nature of one particular residue. PMID: 26645482
- TGF-beta2 induces Grb2 to recruit PI3-K to TGF-RII that activates JNK/AP-1-signaling and augments invasiveness of Theileria-transformed macrophages. PMID: 26511382
- Genetic variation near GRB2 on chromosome 17q25.1 is associated with sight-threatening diabetic retinopathy. PMID: 26188370
- Only monomeric Grb2 is capable of upregulating MAP kinase signalling. The dimeric state is inhibitory to this process. The self-association/dissociation of Grb2 represents a switch that regulates MAP kinase activity and hence controls cancer progression. PMID: 26103942
- The immunoglobulin tail tyrosine motif in the cytoplasmic segments of membrane-bound IgGs acts as the principle signal amplifier by incorporating a Grb2-Btk signaling. PMID: 25413232
- findings show that regions outside of the consensus PxxPxR sites drive the high affinity association of GRB2 with SH3 domain ligands PMID: 26079855
- down-regulated molecule growth factor receptor-bound protein 2 (GRB2) was a prominent node in fourteen cell proliferation-related sub-pathways. PMID: 25659534
- GRB2 was an independent factor in prognosis of esophageal squamous cell carcinoma patients PMID: 25031732
- In addition, GRB2 and UBC were found to be hub nodes in the differentially expressed subnetwork and may be used as potential biomarkers or targets for the diagnosis of bladder cancer. PMID: 25688510
- The N-terminal SH3 domain of Grb2 specifically interacts with UVRAG, unlike the C-terminal SH3 domain. This interaction helps to understand the role of Grb2 in the autophagic maturation of vesicles. PMID: 24882360
- Knockdown of GRB2 suppressed LMTK3-induced CDC42 activation, blocked ITGA5 and ITGB1 expression promoted by the transcription factor serum response factor (SRF), and reduced invasive activity PMID: 24939894
- these results identify Grb2 and Shc as central signaling effectors of Met-driven progression of intestinal epithelial-derived cancers. Notably, they suggest that Grb2 may represent a promising target for the design of novel colorectal cancer therapies. PMID: 24708867
- SGEF interacts with Grb2. PMID: 24399467
- Trafficking of EGFR and Grb2 in living HeLa cells stimulated with low, physiological concentrations of EGFR ligands, was analyzed.. PMID: 24259669
- In neuronal cells, amyloid-beta protein precursor intracellular domain co-localizes with GRB2 in cell compartments. PMID: 24100123
- Recruitment of the adaptor protein Grb2 to EGFR tetramers. PMID: 24697349
- suppression of Grb2 expression in HepG2 cells improved hepatic steatosis, glucose metabolism, oxidative stress, and apoptosis induced by palmitic acid incubation partly though modulating the insulin signaling pathway. PMID: 23771793
- Epigenetic repression of miR-376c accelerated EGF-dependent cell migration through its target GRB2 in HuCCT1 cells. PMID: 23922722
- a central role for adaptor proteins p66Shc and Grb2 in the regulation of ARF1 and ARF6 activation in invasive breast cancer cells. PMID: 24407288
- A deficient lipid rafts recruitment of CD3zeta/ZAP-70/Grb2, and these proteins do not merge with GM1 within the lipid rafts. PMID: 23916875
- EGFR interacts with Grb2 in both constitutive and EGF-dependent manners, whereas the HER3-Grb2 interaction requires the heteromerization of EGFR and HER3. PMID: 23700486
- VP11/12 SFK-binding motifs recruit Lck and the activated Src family kinase then leads (directly or indirectly) to phosphorylation of additional motifs involved in recruiting p85, Grb2, and Shc. PMID: 23946459
- the N-terminal SH3-SH2 region of grb2 protein was in charge of binding with phosphorylated TIGIT. PMID: 23154388
- GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. PMID: 23460737
- Grb2 is thereby recruited into the plexin-B1 receptor complex and, through its SH3 domain, interacts with p190 RhoGAP and mediates RhoA deactivation. PMID: 22505611
- Grb2 exerts constitutive control over the mutually dependent activities of FGFR2 and Shp2. PMID: 23420874
- The discovery of a negative regulatory role for Grb2 reveals that this adaptor acts as a double-edged sword in the regulation of RTK signaling. PMID: 23131845
- The Dok-3/Grb2 protein signal module attenuates Lyn kinase-dependent activation of Syk kinase in B cell antigen receptor microclusters PMID: 23223229
- The results fit with a role of Grb2 in protein aggregation, achieving specificity by multivalent interactions, despite the relatively low affinity of single SH3 interactions. PMID: 23159538
- Grb2 interacts with ADAM12 and is required for ADAM12 internalization. PMID: 22882974
- intracellular Francisella tularensis novicida triggers temporal and early activation of Ras through the SOS2/GrB2/PKCalpha/PKCbetaI quaternary complex; Ras signalling by intracellular F. tularensis is essential for intracellular proliferation in the cytosol PMID: 20618341
- the negative loop on p38 is mediated by c-ABL phosphorylation at tyrosine 105 of the adaptor protein NCK1, while the phosphorylation at tyrosine 209 of GRB2 down-modulates ERK1/2 and JNKs signaling. PMID: 22327338
- T-cell antigen receptor (TCR)-mediated Erk activation requires RasGRP1, but not Grb2/Sos. PMID: 22344067
- Modeling and simulation of aggregation of membrane protein LAT with molecular variability in the number of binding sites for cytosolic Grb2-SOS1-Grb2 PMID: 22396725
- multiple binding sites within Sos1 provide a physical route for Grb2 to hop in a flip-flop manner from one site to the next through facilitated diffusion, and such rapid exchange forms the basis of cooperativity driving bivalent binding of Grb2 to Sos1 PMID: 22360309
- Hypoxia can induce the activation of cytoplasmic FAK, Grb2 and paxillin so as to regulate the migration, survival and proliferation of pulmonary artery smooth cells. PMID: 22094095
- Our data reliably define context-specific and time-dependent networks that form around GRB2 after stimulation, and reveal core and growth factor-selective complexes comprising 90 proteins identified as interacting with GRB2 in HEK293T cells. PMID: 21706016
- ACAP4 protein cooperates with Grb2 protein to orchestrate epidermal growth factor-stimulated integrin beta1 recycling in cell migration PMID: 22027826
- High-resolution crystal structures of Grb2-SH2 domain bound to AICD peptides reveal a unique mode of binding where the peptides assume a noncanonical conformation that is unlike other structures of AICD peptides bound to protein-tyrosine-binding domains PMID: 22001015
- RhoU physically associates with activated EGFR in a GRB2-dependent manner; suppression of GRB2 by RNA interference abrogates the interaction of RhoU with activated EGFR. PMID: 21508312
显示更多
收起更多
-
亚细胞定位:Nucleus. Cytoplasm. Endosome. Golgi apparatus.
-
蛋白家族:GRB2/sem-5/DRK family
-
数据库链接:
HGNC: 4566
OMIM: 108355
KEGG: hsa:2885
STRING: 9606.ENSP00000339007
UniGene: Hs.444356
Most popular with customers
-
Recombinant Human C5a anaphylatoxin chemotactic receptor 1 (C5AR1)-VLPs (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Early activation antigen CD69 (CD69), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)