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  4. Recombinant Human Guanine nucleotide-binding protein G (olf) subunit alpha (GNAL)

Recombinant Human Guanine nucleotide-binding protein G (olf) subunit alpha (GNAL)

  • 中文名称：
    人GNAL重组蛋白
  • 货号：
    CSB-YP009592HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GNAL重组蛋白
  • 货号：
    CSB-EP009592HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GNAL重组蛋白
  • 货号：
    CSB-EP009592HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GNAL重组蛋白
  • 货号：
    CSB-BP009592HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GNAL重组蛋白
  • 货号：
    CSB-MP009592HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Adenylate cyclase stimulating G alpha protein; olfactory type; Adenylate cyclase-stimulating G alpha protein; DYT25; G alpha olf; Gnal; GNAL_HUMAN; Guanine nucleotide binding protein (G protein); alpha activating activity polypeptide; olfactory type; Guanine nucleotide binding protein (G protein); alpha stimulating activity polypeptide; olfactory type ; Guanine nucleotide binding protein G(olf) subunit alpha ; Guanine nucleotide-binding protein G(olf) subunit alpha; olfactory type
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-381
  • 氨基酸序列
    MGCLGGNSKT TEDQGVDEKE RREANKKIEK QLQKERLAYK ATHRLLLLGA GESGKSTIVK QMRILHVNGF NPEEKKQKIL DIRKNVKDAI VTIVSAMSTI IPPVPLANPE NQFRSDYIKS IAPITDFEYS QEFFDHVKKL WDDEGVKACF ERSNEYQLID CAQYFLERID SVSLVDYTPT DQDLLRCRVL TSGIFETRFQ VDKVNFHMFD VGGQRDERRK WIQCFNDVTA IIYVAACSSY NMVIREDNNT NRLRESLDLF ESIWNNRWLR TISIILFLNK QDMLAEKVLA GKSKIEDYFP EYANYTVPED ATPDAGEDPK VTRAKFFIRD LFLRISTATG DGKHYCYPHF TCAVDTENIR RVFNDCRDII QRMHLKQYEL L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I received protein information from you a couple of weeks ago regarding CSB-EP009592HU, but I stressed that GST-tag was one of the only tags that I would be able to use for my experiment.
Do you know if CSB-EP009592HU would have a high percentage chance of successfully being expressed with a GST-tag?
I may be able to use a His-sumo tag, but GST-tag would be the best.
What alternate tags would you use if GST wasn’t possible?

A:
Thanks for your inquiry again !
In theory, the success rate of successfully express this protein with GST tag is 95.25%, so please remark your requirement when placing the order, we will preferentially try this tag. If finally the GST-tagged protein is failed to express, we will try many kinds of other tags, for example: N-terminal 6xHis-SUMO-tagged, N-terminal 6xHis-GST-tagged,N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged and so on.
PS: If you don't need the C-terminal Tag, please inform us in advance, we can successfully remove the C-terminal tag.
In addition, we recommend you to provide some more options besides the GST tag, so we can provide more appropriate recommendation.

靶点详情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters.
  • 基因功能参考文献：
    1. GNAL mutation may represent one of the rare causative genetic factors of isolated laryngeal dystonia. PMID: 27093447
    2. GNAL mutations are not a common cause of dystonia in the Brazilian population PMID: 26810727
    3. We report a novel GNAL mutation in Italian family with adult-onset, dominantly-inherited dystonia PMID: 26725140
    4. Mutations in the GNAL gene may not be a common cause of isolated dystonia in the Chinese population. PMID: 26365774
    5. This study demonstrated that Mutations in GNAL may cause Dystonia. PMID: 25847575
    6. identified two novel GNAL mutations: one heterozygous missense variant in GNAL exon 4, c.289A>G. PMID: 25382112
    7. The findings of this study further support GNAL as causative gene in adult-onset isolated dystonia. PMID: 24408567
    8. This study identified a novel likely disease-causing GNAL mutation in a Serbian patient with cervical dystonia and a classical DYT25 phenotype. PMID: 24729450
    9. Primary dystonia in the Amish-Mennonites is genetically diverse and includes not only the THAP1 indel founder mutation but also different mutations in THAP1 and GNAL as well as the TOR1A GAG deletion. PMID: 24500857
    10. GNAL variants seem to be a rare cause of primary torsion dystonia in our mainly sporadic German sample. PMID: 24151159
    11. Our own data suggest that GNAL mutations do not represent a common cause of dystonia in the U.K. population. PMID: 24222099
    12. The GNAL dystonia gene is central for striatal responses to dopamine (DA) and is a component of a molecular pathway already implicated in DOPA-responsive dystonia (DRD). PMID: 24144882
    13. GNAL mutations potentially increase ethnic susceptibility to movement disorders induced by dopamine antagonists. PMID: 24535567
    14. Mutations in GNAL gene can cause adult-onset primary dystonia in Chinese patients PMID: 23759320
    15. Familial adult-onset primary dystonia can result from mutations in GNAL. PMID: 23449625
    16. Mutations in GNAL cause primary torsion dystonia. PMID: 23222958
    17. these findings provide important clues to understanding physiological functions of XLGalpha(olf). PMID: 22120635
    18. investigation of whether polymorphisms in the alpha subunit of the Golf gene (A-->G in intron 3, and T-->G in intron 10) are associated with major depression; additionally tested for a parent-of-origin effect in separated gender groups PMID: 11901355
    19. promotes cellular invasion, survival and neuroendocrine differentiation in colon, kidney and prostate epithelial cells PMID: 12037684
    20. No support for the hypothesis that the olfactory G-protein gene is a major susceptibility factor for bipolar disorders. PMID: 12782961
    21. identified a transcriptional variant of the GNAL gene in chromosome 18p11.2 in susceptibility to bipolar disorder and schizophrenia PMID: 16044173
    22. Galpha(olf) variant XLGalpha(olf) interacts with the human adenosine A2A receptor PMID: 16818375
    23. We hypothesized that the G(s)-like subunit Galpha(olf), expressed in D1-rich areas of the brain, contributes to the genetic susceptibility of ADHD. We examined the inheritance pattern of 12 GNAL polymorphisms in 258 nuclear families. PMID: 17166517
    24. This study provides important clues toward understanding physiological functions of XLGalpha(olf). PMID: 19245791

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  • 相关疾病：
    Dystonia 25 (DYT25)
  • 蛋白家族：
    G-alpha family, G(s) subfamily
  • 组织特异性：
    Detected in olfactory neuroepithelium, brain, testis, and to a lower extent in retina, lung alveoli, spleen. Trace amounts where seen in kidney, adrenal gland and liver. Found to be expressed in all the insulinomas examined.
  • 数据库链接：

    HGNC: 4388

    OMIM: 139312

    KEGG: hsa:2774

    STRING: 9606.ENSP00000334051

    UniGene: Hs.136295