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  4. Recombinant Human Guanine nucleotide-binding protein subunit alpha-11 (GNA11)

Recombinant Human Guanine nucleotide-binding protein subunit alpha-11 (GNA11)

  • 货号：
    CSB-YP009583HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009583HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009583HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009583HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009583HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    G alpha-11; G-protein subunit alpha-11; GA11; GNA11; GNA11_HUMAN; guanine nucleotide binding protein (G protein); alpha 11 (Gq class); Guanine nucleotide-binding protein G(y) subunit alpha; Guanine nucleotide-binding protein subunit alpha-11; guanine nucleotide-binding protein; alpha 11; guanine nucleotide-binding protein; Gq class; GNA11
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-359
  • 氨基酸序列
    MTLESMMACC LSDEVKESKR INAEIEKQLR RDKRDARREL KLLLLGTGES GKSTFIKQMR IIHGAGYSEE DKRGFTKLVY QNIFTAMQAM IRAMETLKIL YKYEQNKANA LLIREVDVEK VTTFEHQYVS AIKTLWEDPG IQECYDRRRE YQLSDSAKYY LTDVDRIATL GYLPTQQDVL RVRVPTTGII EYPFDLENII FRMVDVGGQR SERRKWIHCF ENVTSIMFLV ALSEYDQVLV ESDNENRMEE SKALFRTIIT YPWFQNSSVI LFLNKKDLLE DKILYSHLVD YFPEFDGPQR DAQAAREFIL KMFVDLNPDS DKIIYSHFTC ATDTENIRFV FAAVKDTILQ LNLKEYNLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C. Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs).
  • 基因功能参考文献：
    1. Results show that postzygotic mosaicism for GNA11 mutations causes an overlapping phenotypic spectrum of vascular and melanocytic birthmarks. PMID: 28083870
    2. GNAQ/GNA11Q209 mutations characterized a distinct, albeit uncommon subtype of non-uveal melanoma (0.5-1%). These mutations were essentially melanoma-specific, occurred in all subtypes of this disease (including cutaneous, mucosal, uveal, and unknown primary), and were mutually exclusive with other common melanoma mutations. PMID: 27089234
    3. GNA11 mutations are associated with extremity capillary malformations causing overgrowth. PMID: 28120216
    4. There were no significant differences in the prevalence of GNAQ and GNA11 mutations between patients with or without metastatic disease PMID: 28444874
    5. a novel germline gain-of-function Galpha11 mutation, Val340Met, causing Autosomal Dominant Hypocalcemia Type 2 demonstrates the importance of the Galpha11 C-terminal region for G-protein function and CaSR signal transduction. PMID: 26818911
    6. studies have identified a third Galpha11 mutation (Thr54Met) causing Familial Hypocalciuric Hypercalcemia Type 2 and reveal a critical role for the Galpha11 interdomain interface in CaSR signaling and Ca(2+) o homeostasis. PMID: 26729423
    7. Adenocarcinomas or adenomas derived from pigmented ciliary epithelium is distinguished from uveal melanoma by the absence of SOX10 expression and presence of the BRAF V600E mutation. PMID: 29059311
    8. Mutations in GNAQ and GNA11 genes in Greek uveal melanoma population present frequencies that qualify them as potential targets for customized therapy. PMID: 28982892
    9. Sporadic melanotic schwannoma with overlapping features of melanocytoma bearing a GNA11 mutation in an adolescent girl. PMID: 28012237
    10. we find iris melanomas to be related genetically to choroidal and ciliary body melanomas, frequently harboring GNAQ, GNA11, and EIF1AX mutations. PMID: 28700778
    11. GNAQ and GNA11 mutations occur frequently in mucosal melanoma and may be a prognostic factor for MM. Our data implicate that GNAQ/11 may be potential targets for targeted therapy of mucosal melanoma. PMID: 27498141
    12. CGRP family of receptors displays both ligand- and RAMP-dependent signaling bias among the Galphas, Galphai, and Galphaq/11 pathways. PMID: 27566546
    13. The identified GNA11 mutation results in biochemical abnormalities typical for autosomal dominant hypocalcemia. Additional features, including short stature and early intracranial calcifications, cosegregated with the mutation. PMID: 27334330
    14. In 33 CASR-negative patients with suspected FHH, Data found two (~6%) with a mutation in AP2S1 (p.Arg15Leu and p.Arg15His). Family screening confirmed the genotype-phenotype correlations. Data did not identify any pathogenic mutations in GNA11. PMID: 27913609
    15. Driver mutations are rare in mutational hotspots of BRAF, NRAS, KIT, and GNAQ/GNA11 in oral mucosal melanoma. PMID: 26399561
    16. These findings demonstrate that CaSR-targeted compounds can rectify signaling disturbances caused by germline and somatic Galpha11 mutations, which respectively lead to calcium disorders and tumorigenesis; and that ADH2-causing Galpha11 mutations induce non-constitutive alterations in MAPK signaling. PMID: 26994139
    17. Mutations in GNA11 gene is associated with malignant uveal melanoma. PMID: 26462151
    18. extensive dermal melanocytosis and phakomatosis pigmentovascularis are associated with activating mutations in GNA11 and GNAQ, genes that encode Galpha subunits of heterotrimeric G proteins PMID: 26778290
    19. Melanomas associated with blue nevi or mimicking cellular blue nevi commonly have GNA11 mutations. PMID: 26645730
    20. Distribution of GNAQ and GNA11 Mutation Signatures in Uveal Melanoma Points to a Light Dependent Mutation Mechanism. PMID: 26368812
    21. Oncogenic GNA11 mutation is associated with uveal melanoma. PMID: 25280020
    22. Involved in the MAPK/ERK, PI3K/AKT, and GNAQ/11 pathways. PMID: 25695059
    23. review discusses the multiple activated signaling targets downstream of mutant GNAQ and GNA11 in uveal melanoma, including MEK, PI3-kinase/Akt, protein kinase C, and YAP PMID: 25304237
    24. Identification of Distinct Conformations of the Angiotensin-II Type 1 Receptor Associated with the Gq/11 Protein Pathway and the beta-Arrestin Pathway Using Molecular Dynamics Simulations. PMID: 25934394
    25. melanopsin is a powerful optogenetic tool for the investigation of spatial and temporal aspects of Gq signalling in cardiovascular research PMID: 24576953
    26. Our findings indicate that the germline gain-of-function mutation of GNA11 is a cause of autosomal dominant hypoparathyroidism and implicate a novel role for GNA11 in skeletal growth. PMID: 24823460
    27. Metastatic uveal melanoma with GNAQ or GNA11 mutations is responsive to PKC inhibitors. PMID: 24141786
    28. There was a significant association of GNA11 mutation status with metastatic status in uveal melanoma. PMID: 24970262
    29. Increasing Galpha11 protein expression in osteoblasts can alter gene expression and result in a dual mechanism of trabecular bone loss. PMID: 24308950
    30. This study identifies HRAS mutations in deep penetrating nevi. The presence of HRAS mutations and absence of GNAQ or GNA11 mutations in deep penetrating nevi suggests classification of these nevi within the Spitz rather than the blue nevus category. PMID: 23599145
    31. the PECAM-1.Galphaq/11 mechanosensitive complex contains an endogenous heparan sulfate proteoglycan with HS chains that is critical for junctional complex assembly and regulating the flow response PMID: 24497640
    32. There is a specific Egr-1 binding site at nt-475/-445. Egr-1 evoked an increased GNA11 transcription. PMID: 23802749
    33. Mutant alleles of the GNA11 or GNAQ genes, which are highly specific for uveal melanoma, were identified in cell-free DNA of 9 of 22 (41%) patients. PMID: 23634288
    34. In primary melanocytic tumours, GNA11 and N-RAS mutations represent a mechanism of MAPK pathway activation alternative to the common GNAQ mutations PMID: 22758774
    35. GNAQ and GNA11 mutations are, in equal matter, not associated with uveal melanoma patient outcome. PMID: 23778528
    36. Letter/Case Report: role of GNAQ/GNA11 mutational analysis in management of choroidal melanoma metastatic to the contralateral orbit. PMID: 23572068
    37. Galpha11 mutants with loss of function cause familial hypocalciuric hypercalcemia type 2, and Galpha11 mutants with gain of function cause a clinical disorder designated as autosomal dominant hypocalcemia type 2. PMID: 23802516
    38. Genomewide linkage analysis, combined with whole-exome sequencing, revealed two different heterozygous mutations affecting Galpha11 as novel causes of autosomal dominant isolated hypoparathyroidism. PMID: 23802536
    39. The vast majority of primary large uveal melanomas harbor mutually-exclusive mutations in GNAQ or GNA11, but very rarely have the oncogenic mutations that are reported commonly in other cancers. PMID: 22977135
    40. The results expand the spectrum of GNA11 mutations that may occur in melanocytomas. PMID: 22307269
    41. The presumed association of TRH-R with G(q/11)alpha proteins in plasma membranes was verified by RNAi experiments PMID: 22240728
    42. [review] Gq transgene activation mediates cardiac hypertrophy in vivo in response to pressure overload; transgenic mice with cardiac-specific deletion of Galphaq family proteins show no ventricular hypertrophy in response to pressure overload PMID: 20531218
    43. The DNA obtained is of sufficient quality to carry out genotyping for markers on chromosome 3, 6 and 8, as well as screening for somatic mutations in GNAQ and GNA11 genes. PMID: 21945171
    44. Regulation of the epithelial Na+ channel by the RH domain of G protein-coupled receptor kinase, GRK2, and Galphaq/11. PMID: 21464134
    45. Benign and malignant blue nevi harbor frequent mutations in the Galphaq class of G-protein alpha subunits, Gnaq and Gna11 proteins. PMID: 21366456
    46. Of the uveal melanomas we analyzed, 83% had somatic mutations in GNAQ or GNA11. PMID: 21083380
    47. the C-terminal domain participates intimately in the efficacy of B1R and B2R G(q/11) coupling by contributing both positive and negative regulatory epitopes. PMID: 12130679
    48. GNA11 is involved in signalling of gonadotropin-releasing hormone receptor, which negatively regulates cell growth. Down-regulation is suggested to be involved in human breast cancers. PMID: 12759536
    49. a new signaling pathway by which G alpha(q/11)-coupled receptors specifically induce Rho signaling through a direct interaction of activated G alpha(q/11) subunits with p63RhoGEF. PMID: 15632174
    50. regulation of the PLC pathway through the PTH1R is significantly increased by elevating expression of G(11)alpha in osteoblastic cells. PMID: 15693018

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  • 相关疾病：
    Hypocalciuric hypercalcemia, familial 2 (HHC2); Hypocalcemia, autosomal dominant 2 (HYPOC2)
  • 亚细胞定位：
    Cell membrane; Lipid-anchor. Cytoplasm. Note=In testicular cells, expressed exclusively in the cytoplasm.
  • 蛋白家族：
    G-alpha family, G(q) subfamily
  • 组织特异性：
    Expressed in testis.
  • 数据库链接：

    HGNC: 4379

    OMIM: 139313

    KEGG: hsa:2767

    STRING: 9606.ENSP00000078429

    UniGene: Hs.650575