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  4. Recombinant Human Hairy/enhancer-of-split related with YRPW motif protein 1 (HEY1)

Recombinant Human Hairy/enhancer-of-split related with YRPW motif protein 1 (HEY1)

  • 中文名称：
    人HEY1重组蛋白
  • 货号：
    CSB-YP896909HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人HEY1重组蛋白
  • 货号：
    CSB-EP896909HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人HEY1重组蛋白
  • 货号：
    CSB-EP896909HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人HEY1重组蛋白
  • 货号：
    CSB-BP896909HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人HEY1重组蛋白
  • 货号：
    CSB-MP896909HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HEY1
  • Uniprot No.：
  • 别名：
    Basic helix loop helix protein OAF1; bHLHb31; Cardiovascular helix loop-helix factor 2; Cardiovascular helix-loop-helix factor 2; CHF-2; CHF2; Class B basic helix-loop-helix protein 31; Hairy and enhancer of split related protein 1; Hairy and enhancer of split-related protein 1; Hairy enhancer-of-split related with YRPW motif protein 1; Hairy related transcription factor 1; Hairy-related transcription factor 1; Hairy/enhancer of split related with YRPW motif 1; Hairy/enhancer-of-split related with YRPW motif protein 1; HERP2; HES related repressor protein 1; HES-related repressor protein 1; HES-related repressor protein 2; HESR-1; HESR1; HEY 1; hey1; HEY1_HUMAN; hHRT1; HRT-1; HRT1; OAF1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-304
  • 氨基酸序列
    MKRAHPEYSS SDSELDETIE VEKESADENG NLSSALGSMS PTTSSQILAR KRRRGIIEKR RRDRINNSLS ELRRLVPSAF EKQGSAKLEK AEILQMTVDH LKMLHTAGGK GYFDAHALAM DYRSLGFREC LAEVARYLSI IEGLDASDPL RVRLVSHLNN YASQREAASG AHAGLGHIPW GTVFGHHPHI AHPLLLPQNG HGNAGTTASP TEPHHQGRLG SAHPEAPALR APPSGSLGPV LPVVTSASKL SPPLLSSVAS LSAFPFSFGS FHLLSPNALS PSAPTQAANL GKPYRPWGTE IGAF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3. Involved in the regulation of liver cancer cells self-renewal.
  • 基因功能参考文献：
    1. Simulation of HEY1 Ser-68 phosphorylation prevents its interaction with p53, RPL11 and MDM2 and abolishes HEY1 migration to nucleolar caps upon ribosomal stress. Our findings uncover a novel mechanism for cross-talk between Notch signalling and nucleolar stress PMID: 27129302
    2. HGF-induced FRA1 activation is associated with the fibrosis-dependent development of Hepatocellular Carcinoma. PMID: 27134167
    3. Expression of Hey1 induces translocation of FBXO45 into the nucleus. PMID: 26068074
    4. Overexpression of HEY1 and HEY2 in esophageal squamous cell carcinoma (ESCC) is correlated to different indices of poor prognosis, and it is extrapolated that such overexpression is important in progression and development of ESCC tumorigenesis. PMID: 25361534
    5. bone morphogenic proteins within the serum of cell culture medium are potent inducers of endothelial Hey1 and Hey2 gene expression within the first few hours after medium change PMID: 25799559
    6. PML degradation mechanism through Notch/Hey1-induced repression of the PML deubiquitinase USP11 involved in brain tumour pathogenesis PMID: 24487962
    7. MMP9 was a downstream effector of HEY1 that promotes the invasion of osteosarcoma cells. Knockdown of HEY1 decreased the expression of MMP9. PMID: 25742474
    8. In prostate tumors, the overexpression of PTOV1 was associated with decreased expression of HEY1 and HES1, and this correlation was significant in metastatic lesions. PMID: 24684754
    9. a new HRD1-associated membrane protein named HERP2, which is homologous to the previously identified HRD1 partner HERP1. Despite sequence homology, HERP2 is constitutively expressed in cells, whereas HERP1 is highly induced by ER stress. PMID: 24366871
    10. HEY1 was overexpressed in colorectal cancer, and correlated with perineural and vascular invasion and lymph node metastasis as well as poorer prognosis. PMID: 23900217
    11. Data show that the periodontal ligament-derived mesenchymal stem cells (hPDLSCs) on a Jagged-1-modified surface had increased expression of Notch signaling target genes, Hes-1 and Hey-1, suggesting the involvement of Notch signaling in hPDLSCs. PMID: 23379739
    12. These data further support a central role for Notch signalling in pancreatic cancer and suggest that nuclear expression of Notch3 and its target gene, HEY-1, merit validation in biomarker panels for diagnosis, prognosis and treatment efficacy. PMID: 23226563
    13. Data suggest that HEs-1/Hey-1 transcriptional modulation of insulin degrading enzyme may impact amyloid beta metabolism by providing a functional link between Notch signaling and the amyloidogenic pathway. PMID: 22036964
    14. In this study, we analyzed the effects of HESR1, -2, and -3 on DAT1 expression in human neuroblastoma SH-SY5Y cells PMID: 21290414
    15. In transgenic mice Hey1 overexpression results in distinct progressive osteopenia and inhibition of osteoblasts ex vivo, an effect apparently dominant to a mild inhibition of osteoclasts. PMID: 19857617
    16. Data show that HEY1 Leu94Met gene polymorphism converts HEY1 from an androgen receptor corepressor to an androgen receptor co-activator, and also abolishes HEY1-mediated activation of p53. PMID: 19802006
    17. Hey1 inhibits myogenesis by associating with and repressing expression of key myogenic targets. PMID: 19917614
    18. HESR-1 may be involved in the phenotypic changes that characterize the progression from EC proliferation and migration to network formation and quiescence PMID: 12453432
    19. These results define a novel regulatory pathway linking the transcription factors c-Jun, HERP2, and GATA-1. PMID: 15314183
    20. Hey1 is excluded from the nucleus in most human prostate cancers, raising the possibility that an abnormal Hey1 subcellular distribution may have a role in the aberrant hormonal responses observed in prostate cancer. PMID: 15684393
    21. Hesr1 down-regulates DAT1 gene expression with the 3' non-coding region, the negative regulations depend on bHLH and the Orange domain of the molecule, but not the YRPW motif. PMID: 15749835
    22. HESR1 seems to inhibit the vessel-promoting effects of VEGF, shift endothelial cells from a proliferative state to a quiescent state, and restore normal vessel structures. PMID: 17028039
    23. Present a novel mechanism by which a balance between Notch-1/-2/-4 signaling, via CBF-1, and HRT-1/-2 activity determines the expression of smooth muscle differentiation markers including actin. PMID: 18239137
    24. Data imply a role for HEY1 in the progression of glioblastoma multiforme, and suggest that HEY1 may be a therapeutic target for glioblastoma patients. PMID: 18363832
    25. Hey1 and Runx2 were shown to act synergistically in BMP9-induced osteogenic differentiation, and Runx2 expression significantly decreased in the absence of Hey1, suggesting that Runx2 may function downstream of Hey1 PMID: 18986983
    26. Hey1 represses the KSHV RTA promoter, and mSin3A interacts with Hey1 and that this interaction is abolished in the presence of RTA. PMID: 19369342
    27. Declines in HEY1 are associated with pulmonary fibrosis and hypertension. PMID: 17496152
    28. A similar gene in mouse regulates cardiovascular development PMID: 15680351

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    HEY family
  • 组织特异性：
    Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
  • 数据库链接：

    HGNC: 4880

    OMIM: 602953

    KEGG: hsa:23462

    STRING: 9606.ENSP00000338272

    UniGene: Hs.234434